Most Anti-BrdU Antibodies React with 2′-Deoxy-5-Ethynyluridine - The Method for the Effective Suppression of This Cross-Reactivity

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Liboska, Radek ^a   Ligasová, Anna ^b,c   Strunin, Dmytro ^b,c   Rosenberg, Ivan ^a   Koberna, Karel ^b,c  

^a INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^b INSTITUTE OF BIOPHYSICS   (Czech Republic)

^c INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

5 ETHYNYLDEOXYURIDINE; ANTIBODY; BROXURIDINE; CELL NUCLEUS DNA; COPPER ION; DEOXYURIDINE DERIVATIVE; FLUORESCENT DYE; FLUOROCHROME; HYDROCHLORIC ACID; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ARTICLE; BIOTINYLATION; CLICK CHEMISTRY; CONTROLLED STUDY; DNA SYNTHESIS; HUMAN; HUMAN CELL;

ANTIBODIES; ANTIBODY AFFINITY; BIOLOGICAL TRANSPORT; BIOTINYLATION; BROMODEOXYURIDINE; CROSS REACTIONS; DEOXYURIDINE; DNA; FLUORESCENT DYES; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; STAINING AND LABELING;

EID: 84871338630     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0051679     Document Type: Article

References (16)

1. Dimitrova DS, Berezney R, (2002) The spatio-temporal organization of DNA replication sites is identical in primary, immortalized and transformed mammalian cells. J Cell Sci 115: 4037-4051.
2. Kennedy BK, Barbie DA, Classon M, Dyson N, Harlow E, (2000) Nuclear organization of DNA replication in primary mammalian cells. Genes Dev 14: 2855-2868.
3. Aten JA, Bakker PJ, Stap J, Boschman GA, Veenhof CH, (1992) DNA double labelling with IdUrd and CldUrd for spatial and temporal analysis of cell proliferation and DNA replication. Histochem J 24: 251-259.
4. Merrick CJ, Jackson D, Diffley JF, (2004) Visualization of altered replication dynamics after DNA damage in human cells. J Biol Chem 279: 20067-20075.
5. Seiler JA, Conti C, Syed A, Aladjem MI, Pommier Y, (2007) The intra-S-phase checkpoint affects both DNA replication initiation and elongation: single-cell and-DNA fiber analyses. Mol Cell Biol 27: 5806-5818.
6. Ageno M, Dore E, Frontali C, (1969) The alkaline denaturation of DNA. Biophys J 9: 1281-1311.
7. Jackson DA, Pombo A, (1998) Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells. J Cell Biol 140: 1285-1295.
8. Stanojcic S, Lemaitre JM, Brodolin K, Danis E, Mechali M, (2008) In Xenopus egg extracts, DNA replication initiates preferentially at or near asymmetric AT sequences. Mol Cell Biol 28: 5265-5274.
9. Ligasová A, Strunin D, Liboska R, Rosenberg I, Koberna K (2012) Atomic Scissors: A New Method of Tracking the 5-Bromo-2′-Deoxyuridine-Labeled DNA in Situ. PLoS One: In press.
10. Salic A, Mitchison TJ, (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105: 2415-2420.
11. Wu JC, Wang DX, Huang ZT, Wang MX, (2010) Synthesis of diverse N,O-bridged calix[1]arene[4]pyridine-C(60) dyads and triads and formation of intramolecular self-inclusion complexes. J Org Chem 75: 8604-8614.
12. Behrens CH, Sharpless KB, (1985) Selective Transformations of 2,3-Epoxy Alcohols and Related Derivatives- Strategies for Nucleophilic-Attack at Carbon-3 or Carbon-2. J Org Chem 50: 5696-5704.
13. Ligasová A, Koberna K, (2010) In situ reverse transcription: the magic of strength and anonymity. Nucleic Acids Res 38: e167.
14. Bradford JA, Clarke ST, (2011) Dual-pulse labeling using 5-ethynyl-2′-deoxyuridine (EdU) and 5-bromo-2′-deoxyuridine (BrdU) in flow cytometry. Curr Protoc Cytom 55: 1-15.
15. Barr PJ, Nolan PA, Santi DV, Robins MJ, (1981) Inhibition of thymidylate synthetase by 5-alkynyl-2′-deoxyuridylates. J Med Chem 24: 1385-1388.
16. Qu D, Wang G, Wang Z, Zhou L, Chi W, et al. (2011) 5-Ethynyl-2′-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells. Anal Biochem 417: 112-121.