Targeted manipulation of heterochromatin rescues MeCP2 Rett mutants and re-establishes higher order chromatin organization

Nucleic Acids Research

Volumn 40, Issue 22, 2012, Pages

  Casas Delucchi, Corella S ^a   Becker, Annette ^a   Bolius, Janine J ^a   Cristina Cardoso, M ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

METHYL CPG BINDING PROTEIN 2; NUCLEAR FACTOR;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CHROMATIN ASSEMBLY AND DISASSEMBLY; CHROMATIN STRUCTURE; GENE MUTATION; GENE TARGETING; GENETIC MANIPULATION; HETEROCHROMATIN; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RETT SYNDROME; VALIDATION PROCESS;

ANIMALS; CELLS, CULTURED; DNA-BINDING PROTEINS; GREEN FLUORESCENT PROTEINS; HETEROCHROMATIN; HUMANS; METHYL-CPG-BINDING PROTEIN 2; MICE; MICROSCOPY, FLUORESCENCE; MUTATION; RECOMBINANT FUSION PROTEINS; RETT SYNDROME; SINGLE-DOMAIN ANTIBODIES;

MAMMALIA;

EID: 84871231720     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gks784     Document Type: Article

References (20)

1. Nan, X., Meehan, R. R. and Bird, A. (1993) Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2. Nucleic Acids Res., 21, 4886-4892.
2. Nan, X., Campoy, F. J. and Bird, A. (1997) MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin. Cell, 88, 471-481.
3. Brero, A., Easwaran, H. P., Nowak, D., Grunewald, I., Cremer, T., Leonhardt, H. and Cardoso, M. C. (2005) Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation. J. Cell Biol., 169, 733-743.
4. Georgel, P. T., Horowitz-Scherer, R. A., Adkins, N., Woodcock, C. L., Wade, P. A. and Hansen, J. C. (2003) Chromatin compaction by human MeCP2. Assembly of novel secondary chromatin structures in the absence of DNA methylation. J. Biol. Chem., 278, 32181-32188.
5. Nikitina, T., Ghosh, R. P., Horowitz-Scherer, R. A., Hansen, J. C., Grigoryev, S. A. and Woodcock, C. L. (2007) MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. J. Biol. Chem., 282, 28237-28245.
6. Nikitina, T., Shi, X., Ghosh, R. P., Horowitz-Scherer, R. A., Hansen, J. C. and Woodcock, C. L. (2007) Multiple modes of interaction between the methylated DNA binding protein MeCP2 and chromatin. Mol. Cell Biol., 27, 864-877.
7. Amir, R. E., Van den Veyver, I. B., Wan, M., Tran, C. Q., Francke, U. and Zoghbi, H. Y. (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat. Genet., 23, 185-188.
8. Chahrour, M. and Zoghbi, H. Y. (2007) The story of Rett syndrome: from clinic to neurobiology. Neuron, 56, 422-437.
9. Agarwal, N., Becker, A., Jost, K. L., Haase, S., Thakur, B. K., Brero, A., Hardt, T., Kudo, S., Leonhardt, H. and Cardoso, M. C. (2011) MeCP2 Rett mutations affect large scale chromatin organization. Hum. Mol. Genet., 20, 4187-4195.
10. Kudo, S., Nomura, Y., Segawa, M., Fujita, N., Nakao, M., Schanen, C. and Tamura, M. (2003) Heterogeneity in residual function of MeCP2 carrying missense mutations in the methyl CpG binding domain. J. Med. Genet., 40, 487-493.
11. Lindhout, B. I., Fransz, P., Tessadori, F., Meckel, T., Hooykaas, P. J. and van der Zaal, B. J. (2007) Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins. Nucleic Acids Res., 35, e107.
12. Rothbauer, U., Zolghadr, K., Muyldermans, S., Schepers, A., Cardoso, M. C. and Leonhardt, H. (2008) A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteins. Mol. Cell. Proteomics, 7, 282-289.
13. Rothbauer, U., Zolghadr, K., Tillib, S., Nowak, D., Schermelleh, L., Gahl, A., Backmann, N., Conrath, K., Muyldermans, S., Cardoso, M. C. et al. (2006) Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat. Methods, 3, 887-889.
14. Berkovich, E., Monnat, R. J. Jr and Kastan, M. B. (2007) Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair. Nat. Cell Biol., 9, 683-690.
15. Casas-Delucchi, C. S., Brero, A., Rahn, H. P., Solovei, I., Wutz, A., Cremer, T., Leonhardt, H. and Cardoso, M. C. (2011) Histone acetylation controls the inactive X chromosome replication dynamics. Nat. Commun., 2, 222.
16. Casas-Delucchi, C. S., van Bemmel, J. G., Haase, S., Herce, H. D., Nowak, D., Meilinger, D., Stear, J. H., Leonhardt, H. and Cardoso, M. C. (2012) Histone hypoacetylation is required to maintain late replication timing of constitutive heterochromatin. Nucleic Acids Res., 40, 159-169.
17. Ho, K. L., McNae, I. W., Schmiedeberg, L., Klose, R. J., Bird, A. P. and Walkinshaw, M. D. (2008) MeCP2 binding to DNA depends upon hydration at methyl-CpG. Mol. Cell, 29, 525-531.
18. Ghosh, R. P., Horowitz-Scherer, R. A., Nikitina, T., Gierasch, L. M. and Woodcock, C. L. (2008) Rett syndrome-causing mutations in human MeCP2 result in diverse structural changes that impact folding and DNA interactions. J. Biol. Chem., 283, 20523-20534.
19. Ghosh, R. P., Nikitina, T., Horowitz-Scherer, R. A., Gierasch, L. M., Uversky, V. N., Hite, K., Hansen, J. C. and Woodcock, C. L. (2010) Unique physical properties and interactions of the domains of methylated DNA binding protein 2. Biochemistry, 49, 4395-4410.
20. Littlewood, T. D., Hancock, D. C., Danielian, P. S., Parker, M. G. and Evan, G. I. (1995) A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins. Nucleic Acids Res., 23, 1686-1690.