Maize Ribosome-Inactivating Protein Uses Lys158-Lys161 to Interact with Ribosomal Protein P2 and the Strength of Interaction Is Correlated to the Biological Activities

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Wong, Yuen Ting ^a   Ng, Yiu Ming ^a   Mak, Amanda Nga Sze ^a   Sze, Kong Hung ^b   Wong, Kam Bo ^a   Shaw, Pang Chui ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOSIDASE; LYSINE; RIBOSOME INACTIVATING PROTEIN; RIBOSOME PROTEIN; RIBOSOME PROTEIN P2; SHIGA TOXIN; TRICHOSANTHIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BASE PAIRING; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHEMICAL REACTION; CONTROLLED STUDY; CYTOTOXICITY; DEPURINATION; DISSOCIATION CONSTANT; EMBRYO; HUMAN; HUMAN CELL; HYDROPHOBICITY; IONIC STRENGTH; MAIZE; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; RAT;

EID: 84871192986     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0049608     Document Type: Article

References (35)

1. Girbes T, Ferreras JM, Arias FJ, Stirpe F, (2004) Description, distribution, activity and phylogenetic relationship of ribosome-inactivating proteins in plants, fungi and bacteria. Mini Rev Med Chem 4: 461-76.
2. Stirpe F, (2004) Ribosome-inactivating proteins. Toxicon 44: 371-83.
3. Stirpe F, Battelli MG, (2006) Ribosome-inactivating proteins: progress and problems. Cell Mol Life Sci 63: 1850-66.
4. Lancaster L, Lambert NJ, Maklan EJ, Horan LH, Noller HF, (2008) The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit. Rna 14: 1999-2012.
5. Montanaro L, Sperti S, Mattioli A, Testoni G, Stirpe F, (1975) Inhibition by ricin of protein synthesis in vitro. Inhibition of the binding of elongation factor 2 and of adenosine diphosphate-ribosylated elongation factor 2 to ribosomes. Biochem J 146: 127-31.
6. Fernandez-Puentes C, Vazquez D, (1977) Effects of some proteins that inactivate the eukaryotic ribosome. FEBS Lett 78: 143-6.
7. Brigotti M, Rambelli F, Zamboni M, Montanaro L, Sperti S, (1989) Effect of alpha-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes. Biochem J 257: 723-7.
8. Robertus JD, Monzingo AF, (2004) The structure of ribosome inactivating proteins. Mini Rev Med Chem 4: 477-86.
9. de Virgilio M, Lombardi A, Caliandro R, Fabbrini MS, (2010) Ribosome-inactivating proteins: from plant defense to tumor attack. Toxins (Basel) 2: 2699-737.
10. Suh JK, Hovde CJ, Robertus JD, (1998) Shiga toxin attacks bacterial ribosomes as effectively as eucaryotic ribosomes. Biochemistry 37: 9394-8.
11. Barbieri L, Ciani M, Girbes T, Liu WY, Van Damme EJ, et al. (2004) Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins. FEBS Lett 563: 219-22.
12. Narayanan S, Surendranath K, Bora N, Surolia A, Karande AA, (2005) Ribosome inactivating proteins and apoptosis. FEBS Lett 579: 1324-31.
13. Endo Y, Tsurugi K, (1988) The RNA N-glycosidase activity of ricin A-chain. Nucleic Acids Symp Ser pp. 139-42.
14. Chan DS, Chu LO, Lee KM, Too PH, Ma KW, et al. (2007) Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses. Nucleic Acids Res 35: 1660-72.
15. McCluskey AJ, Poon GM, Bolewska-Pedyczak E, Srikumar T, Jeram SM, et al. (2008) The catalytic subunit of shiga-like toxin 1 interacts with ribosomal stalk proteins and is inhibited by their conserved C-terminal domain. J Mol Biol 378: 375-86.
16. McCluskey AJ, Bolewska-Pedyczak E, Jarvik N, Chen G, Sidhu SS, et al. (2012) Charged and hydrophobic surfaces on the A chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteins. PLoS One 7: e31191.
17. Vater CA, Bartle LM, Leszyk JD, Lambert JM, Goldmacher VS, (1995) Ricin A chain can be chemically cross-linked to the mammalian ribosomal proteins L9 and L10e. J Biol Chem 270: 12933-40.
18. Hudak KA, Dinman JD, Tumer NE, (1999) Pokeweed antiviral protein accesses ribosomes by binding to L3. J Biol Chem 274: 3859-64.
19. Rajamohan F, Ozer Z, Mao C, Uckun FM, (2001) Active center cleft residues of pokeweed antiviral protein mediate its high-affinity binding to the ribosomal protein L3. Biochemistry 40: 9104-14.
20. Ayub MJ, Smulski CR, Ma KW, Levin MJ, Shaw PC, et al. (2008) The C-terminal end of P proteins mediates ribosome inactivation by trichosanthin but does not affect the pokeweed antiviral protein activity. Biochem Biophys Res Commun 369: 314-9.
21. Chiou JC, Li XP, Remacha M, Ballesta JP, Tumer NE, (2008) The ribosomal stalk is required for ribosome binding, depurination of the rRNA and cytotoxicity of ricin A chain in Saccharomyces cerevisiae. Mol Microbiol 70: 1441-52.
22. Chiou JC, Li XP, Remacha M, Ballesta JP, Tumer NE, (2011) Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2. Int J Biochem Cell Biol 43: 1792-801.
23. Chaddock JA, Monzingo AF, Robertus JD, Lord JM, Roberts LM, (1996) Major structural differences between pokeweed antiviral protein and ricin A-chain do not account for their differing ribosome specificity. Eur J Biochem 235: 159-66.
24. Nagasawa Y, Fujii K, Yoshikawa T, Kobayashi Y, Kondo T, (2008) Pokeweed antiviral protein region Gly209-Lys225 is critical for RNA N-glycosidase activity of the prokaryotic ribosome. Phytochemistry 69: 1653-60.
25. Too PH, Ma MK, Mak AN, Wong YT, Tung CK, et al. (2009) The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome. Nucleic Acids Res 37: 602-10.
26. Mak AN, Wong YT, An YJ, Cha SS, Sze KH, et al. (2007) Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site. Nucleic Acids Res 35: 6259-67.
27. Yang Y, Mak AN, Shaw PC, Sze KH, (2010) Solution structure of an active mutant of maize ribosome-inactivating protein (MOD) and its interaction with the ribosomal stalk protein P2. J Mol Biol 395: 897-907.
28. Walsh TA, Morgan AE, Hey TD, (1991) Characterization and molecular cloning of a proenzyme form of a ribosome-inactivating protein from maize. Novel mechanism of proenzyme activation by proteolytic removal of a 2.8-kilodalton internal peptide segment. J Biol Chem 266: 23422-7.
29. Hey TD, Hartley M, Walsh TA, (1995) Maize ribosome-inactivating protein (b-32). Homologs in related species, effects on maize ribosomes, and modulation of activity by pro-peptide deletions. Plant Physiol 107: 1323-32.
30. Chan SH, Hung FS, Chan DS, Shaw PC, (2001) Trichosanthin interacts with acidic ribosomal proteins P0 and P1 and mitotic checkpoint protein MAD2B. Eur J Biochem 268: 2107-12.
31. Spedding G (1990) Ribosomes and Protein Synthesis. Oxford: Oxford University Press. 9-12 p.
32. Melchior WB Jr, Tolleson WH, (2010) A functional quantitative polymerase chain reaction assay for ricin, Shiga toxin, and related ribosome-inactivating proteins. Anal Biochem 396: 204-11.
33. Law SK, Wang RR, Mak AN, Wong KB, Zheng YT, et al. (2010) A switch-on mechanism to activate maize ribosome-inactivating protein for targeting HIV-infected cells. Nucleic Acids Res 38: 6803-12.
34. Li XP, Chiou JC, Remacha M, Ballesta JP, Tumer NE, (2009) A two-step binding model proposed for the electrostatic interactions of ricin a chain with ribosomes. Biochemistry 48: 3853-63.
35. Lapadula WJ, Sanchez-Puerta MV, Ayub MJ, (2012) Convergent evolution led ribosome inactivating proteins to interact with ribosomal stalk. Toxicon 59: 427-32.