Bead affinity chromatography in a temperature-controllable microsystem for biomarker detection

Analytical and Bioanalytical Chemistry

Volumn 404, Issue 8, 2012, Pages 2267-2275

  Koh, Yul ^a   Lee, Bo Rahm ^a   Yoon, Hyo Jin ^a   Jang, Yun Ho ^a   Lee, Yoon Sik ^a   Kim, Yong Kweon ^a   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

Author keywords

Aptamer immobilized bead affinity chromatography; Carcinoembryonic antigen (CEA); MALDI TOF MS; Temperature controllable microfluidics

Indexed keywords

CANCER BIOMARKER DETECTIONS; CANCER MARKERS; CANCER RESEARCH; CARCINOEMBRYONIC ANTIGEN; HUMAN SERUM; MALDI TOF MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHTS; MICRO-REACTOR; MICROBEADS; PROTEIN ANALYSIS; REACTION CHAMBERS; SAMPLE PREPARATION; SINGLE CRYSTALLINE SILICON; TEMPERATURE CONTROL UNIT;

AFFINITY CHROMATOGRAPHY; ANTIGENS; DESORPTION; DISEASES; MASS SPECTROMETRY; PROTEINS; RNA;

MICROSYSTEMS;

BIOLOGICAL MARKER; MACROGOL DERIVATIVE; ORGANOSILICON DERIVATIVE; PEPTIDE; SILANE DERIVATIVE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BLOOD; CHEMISTRY; GENETICS; HUMAN; INSTRUMENTATION; MOLECULAR GENETICS; NEOPLASM; SURFACE PROPERTY; TEMPERATURE;

AMINO ACID SEQUENCE; BIOLOGICAL MARKERS; CHROMATOGRAPHY, AFFINITY; HUMANS; MOLECULAR SEQUENCE DATA; NEOPLASMS; ORGANOSILICON COMPOUNDS; PEPTIDES; POLYETHYLENE GLYCOLS; SILANES; SURFACE PROPERTIES; TEMPERATURE;

EID: 84870906236     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-6380-1     Document Type: Article

References (27)

1. Srinivas PR, Verma M, Zhao YM, Srivastava S (2002) Proteomics for cancer biomarker discovery. Clin Chem 48:1160-1169
2. Cutler P (2003) Protein arrays: the current state-of-the-art. Proteomics 3:3-18
3. Sato K, Tokeshi M, Kimura H, Kitamori T (2001) Determination of carcinoembryonic antigen in human sera by integrated bead bed immunoasay in a microchip for cancer diagnosis. Anal Chem 73:1213-1218
4. Fu ZF, Yan F, Liu H, Yang ZJ, Ju HX (2008) Channel-resolved multianalyte immunosensing system for flow-through chemiluminescent detection of alpha-fetoprotein and carcinoembryonic antigen. Biosens Bioelectron 23:1063-1069
5. Dai Z, Chen J, Yan F, Ju HX (2005) Electrochemical sensor for immunoassay of carcinoembryonic antigen based on thionine monolayer modified gold electrode. Cancer Detect Prev 29:233- 240
6. Lion N, Gellon JO, Jensen H, Girault HH (2003) On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry. J Chromatogr A 1003:11-19
7. Wheeler AR, Moon H, Bird CA, Loo RRO, Kim CJ, Loo JA, Garrell RL (2005) Digital microfluidics with in-line sample purification for proteomics analyses with MALDI-MS. Anal Chem 77:534-540
8. Ekstrom S, Malmstrom J, Wallman L, Lofgren M, Nilsson J, Laurell T, Marko-Varga G (2002) On-chip microextraction for proteomic sample preparation of in-gel digests. Proteomics 2:413-421
9. Lee WC, Lee KH (2004) Applications of affinity chromatography in proteomics. Anal Biochem 324:1-10
10. Firer MA (2001) Efficient elution of functional proteins in affinity chromatography. J Biochem Biophys Methods 49:433- 442
11. Chung WJ, Kim MS, Cho S, Park SS, Kim JH, Kim YK, Kim BG, Lee YS (2005) Microaffinity purification of proteins based on photolytic elution: toward an efficient microbead affinity chromatography on a chip. Electrophoresis 26:694-702
12. Cho S, Lee SH, Chung WJ, Kim YK, Lee YS, Kim BG (2004) Microbead-based affinity chromatography chip using RNA aptamer modified with photocleavable linker. Electrophoresis 25:3730-3739
13. Park ZY, Russell DH (2000) Thermal denaturation: a useful technique in peptide mass mapping. Anal Chem 72:2667-2670
14. Park ZY, Russell DH (2001) Identification of individual proteins in complex protein mixtures by high-resolution, high-mass-accuracy MALDI TOF-mass spectrometry analysis of in-solution thermal denaturation/enzymatic digestion. Anal Chem 73:2558-2564
15. Nguyen T, Pei R, Stojanovic M, Lin Q (2009) An aptamer-based microfluidic device for thermally controlled affinity extraction. Microfluid Nanofluid 6:479-487
16. deMello AJ (2006) Control and detection of chemical reactions in microfluidic systems. Nature 442:394-402
17. Dittrich PS, Manz A (2006) Lab-on-a-chip: microfluidics in drug discovery. Nature Rev Drug Discov 5:210-218
18. Feng XJ, Du W, Luo QM, Liu BF (2009) Microfluidic chip: next-generation platform for systems biology. Anal Chim Acta 650:83-97
19. Oh KW, Ahn CH (2006) A review of microvalves. J Micromech Microeng 16:R13-R39
20. Han SI, Han KH, Frazier AB, Ferrance JP, Landers JP (2009) An automated micro-solid phase extraction device involving integrated \high-pressure microvalves for genetic sample preparation. Biomed Microdevices 11:935-942
21. Freire SLS, Wheeler AR (2006) Proteome-on-a-chip: mirage, or on the horizon? Lab on a Chip 6:1415-1423
22. Ramsey JD, Collins GE (2005) Integrated microfluidic device for solid-phase extraction coupled to micellar electrokinetic chromatography separation. Anal Chem 77:6664-6670
23. Yu C, Davey MH, Svec F, Frechet JMJ (2001) Monolithic porous polymer for on-chip solid-phase extraction and preconcentration prepared by photoinitiated in situ polymerization within a microfluidic device. Anal Chem 73:5088-5096
24. Sim TS, Kim EM, Joo HS, Kim BG, Kim YK (2006) Application of a temperature-controllable microreactor to simple and rapid protein identification using MALDI-TOF MS. Lab on a Chip 6:1056-1061
25. Yoon DS, Lee YS, Lee Y, Cho HJ, Sung SW, Oh KW, Cha J, Lim G (2002) Precise temperature control and rapid thermal cycling in a micromachined DNA polymerase chain reaction chip. J Micromech Microeng 12:813-823
26. Lao AIK, Lee TMH, Hsing IM, Ip NY (2000) Precise temperature control of microfluidic chamber for gas and liquid phase reactions. Sensors Actuators A Phys 84:11-17
27. LeeYJ,HanSR,KimNY,Lee SH, Jeong JS,Lee SW(2012)AnRNA aptamer that binds carcinoembryonic antigen inhibits hepatic metastasis ofcolon cancer cells in mice. Gastroenterology 143(1):155-165