Molecular diagnostic methods for the detection of Mycobacterium tuberculosis resistance: The potential of high-resolution melting analysis

Expert Review of Anti-Infective Therapy

Volumn 10, Issue 10, 2012, Pages 1075-1077

  Lee, Ann Siew Gek ^a,b   Ong, Danny Chee Tiong ^c  

^a DIVISION OF MEDICAL ONCOLOGY   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c Molecular Diagnostics Laboratory   (Singapore)

Author keywords

drug resistance; high resolution melting analysis; molecular diagnostic tests; mutation screening; Mycobacterium tuberculosis

Indexed keywords

AMIKACIN; CAPREOMYCIN; ETHAMBUTOL; ISONIAZID; KANAMYCIN; RIFAMPICIN; TUBERCULOSTATIC AGENT;

AMPLICON; ANTIBIOTIC RESISTANCE; BACTERIAL GENE; BACTERIAL TRANSMISSION; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; CLINICAL LABORATORY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE MUTATION; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; LUNG TUBERCULOSIS; MEDICAL DEVICE; MOLECULAR DIAGNOSIS; MULTIDRUG RESISTANCE; MUTATIONAL ANALYSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RPOB GENE; SENSITIVITY AND SPECIFICITY; TUBERCULOSIS;

ANTITUBERCULAR AGENTS; DNA MUTATIONAL ANALYSIS; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; TUBERCULOSIS;

EID: 84870586624     PISSN: 14787210     EISSN: 17448336     Source Type: Journal    
DOI: 10.1586/eri.12.94     Document Type: Review

References (15)

1. Chegou NN, Hoek KG, Kriel M, Warren RM, Victor TC, Walzl G. Tuberculosis assays: past, present and future. Expert Rev. Anti. Infect. Ther. 9(4), 457-469 (2011).
2. Telenti A, Imboden P, Marchesi F et al. Detection of rifampicin- resistance mutations in Mycobacterium tuberculosis. Lancet 341(8846), 647-650 (1993).
3. Musser JM. Antimicrobial agent resistance in mycobacteria: molecular genetic insights. Clin. Microbiol. Rev. 8(4), 496-514 (1995).
4. Vilchèze C, Jacobs WR Jr. The mechanism of isoniazid killing: clarity through the scope of genetics. Annu. Rev. Microbiol. 61, 35-50 (2007).
5. Hillemann D, Rüsch-Gerdes S, Richter E. Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens. J. Clin. Microbiol. 45(8), 2635-2640 (2007).
6. Huang WL, Chen HY, Kuo YM, Jou R. Performance assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 47(8), 2520-2524 (2009).
7. Ignatyeva O, Kontsevaya I, Kovalyov A et al. Detection of resistance to second-line antituberculosis drugs by use of the genotype MTBDRsl assay: a multicenter evaluation and feasibility study. J. Clin. Microbiol. 50(5), 1593-1597 (2012).
8. Lacoma A, García-Sierra N, Prat C et al. GenoType MTBDRsl for molecular detection of second-line-drug and ethambutol resistance in Mycobacterium tuberculosis strains and clinical samples. J. Clin. Microbiol. 50(1), 30-36 (2012).
9. Said HM, Kock MM, Ismail NA et al. Evaluation of the GenoType® MTBDRsl assay for susceptibility testing of second-line anti-tuberculosis drugs. Int. J. Tuberc. Lung Dis. 16(1), 104-109 (2012).
10. Huang WL, Chi TL, Wu MH, Jou R. Performance assessment of the GenoType MTBDRsl test and DNA sequencing for detection of second-line and ethambutol drug resistance among patients infected with multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 49(7), 2502-2508 (2011).
11. Helb D, Jones M, Story E et al. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48(1), 229-237 (2010).
12. Boehme CC, Nicol M P, Nabeta P et al. Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study. Lancet 377(9776), 1495-1505 (2011).
13. Chang K, Lu W, Wang J et al. Rapid and effective diagnosis of tuberculosis and rifampicin resistance with Xpert MTB/RIF assay: a meta-analysis. J. Infect. 64(6), 580-588 (2012).
14. Ong DC, Yam WC, Siu GK, Lee AS. Rapid detection of rifampicin-and isoniazid-resistant Mycobacterium tuberculosis by high-resolution melting analysis. J. Clin. Microbiol. 48(4), 1047-1054 (2010).
15. Lee AS, Ong DC, Wong JC, Siu GK, Yam WC. High-resolution melting analysis for the rapid detection of fuoroquinolone and streptomycin resistance in Mycobacterium tuberculosis. PLoS One 7, e31934 (2012).