Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development

Nature Protocols

Volumn 7, Issue 12, 2012, Pages 2090-2102

  Chai, Yongping ^a   Li, Wei ^a   Feng, Guoxin ^a   Yang, Yihong ^a   Wang, Xiangming ^a   Ou, Guangshuo ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BLUE FLUORESCENT PROTEIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CAENORHABDITIS ELEGANS; CELL KINETICS; CELL MIGRATION; CLINICAL PROTOCOL; EMBRYO DEVELOPMENT; FLUORESCENCE IMAGING; GROWTH CURVE; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PURIFICATION; TIME LAPSE IMAGING; TRANSGENICS;

EID: 84870576239     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.128     Document Type: Article

References (37)

1. Green, R.A. et al. Expression and imaging of fluorescent proteins in the C. elegans gonad and early embryo. Methods Cell Biol. 85, 179-218 (2008
2. Sulston, J.E. & Horvitz, H.R. Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev. Biol. 56, 110-156 (1977 (Pubitemid 8049332)
3. Sulston, J.E., Schierenberg, E., White, J.G. & Thomson, J.N. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64-119 (1983
4. Ou, G. & Vale, R.D. Molecular signatures of cell migration in C. elegans Q neuroblasts. J. Cell Biol. 185, 77-85 (2009
5. Ou, G., Stuurman, N., D'Ambrosio, M. & Vale, R.D. Polarized myosin produces unequal-size daughters during asymmetric cell division. Science 330, 677-680 (2010
6. Singhvi, A. et al. The Arf GAP CNT-2 regulates the apoptotic fate in C. elegans asymmetric neuroblast divisions. Curr. Biol. 21, 948-954 (2011
7. Li, W. et al. Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell. J. Cell Biol. 197, 27-35 (2012
8. Cheeseman, I.M. & Desai, A. A combined approach for the localization and tandem affinity purification of protein complexes from metazoans. Sci. STKE 2005, pl1 (2005
9. Knobel, K.M., Jorgensen, E.M. & Bastiani, M.J. Growth cones stall and collapse during axon outgrowth in Caenorhabditis elegans. Development 126, 4489-4498 (1999
10. Ou, G., Blacque, O.E., Snow, J.J., Leroux, M.R. & Scholey, J.M. Functional coordination of intraflagellar transport motors. Nature 436, 583-587 (2005 (Pubitemid 41112934)
11. Joshi, P.M., Riddle, M.R., Djabrayan, N.J. & Rothman, J.H. Caenorhabditis elegans as a model for stem cell biology. Dev. Dyn. 239, 1539-1554 (2010
12. Pederson, T. The nucleolus. Cold Spring Harb. Perspect. Biol. 3 pii:a000638 (2011
13. Goldsmith, A.D., Sarin, S., Lockery, S. & Hobert, O. Developmental control of lateralized neuron size in the nematode Caenorhabditis elegans. Neural Dev. 5, 33 (2010
14. Subach, O.M. et al. Conversion of red fluorescent protein into a bright blue probe. Chem. Biol. 15, 1116-1124 (2008
15. Reboul, J. et al. C. elegans ORFeome version 1.1: Experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat. Genet. 34, 35-41 (2003 (Pubitemid 36548788)
16. Reboul, J. et al. Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nat. Genet. 27, 332-336 (2001 (Pubitemid 32201858)
17. Tursun, B., Cochella, L., Carrera, I. & Hobert, O. A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans. PLoS One 4, e4625 (2009
18. Hobert, O. PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans. Biotechniques 32, 728-730 (2002 (Pubitemid 34298696)
19. Mello, C. & Fire, A. DNA transformation. Methods Cell Biol. 48, 451-482 (1995
20. Frokjaer-Jensen, C. et al. Single-copy insertion of transgenes in Caenorhabditis elegans. Nat. Genet. 40, 1375-1383 (2008
21. Bloom, L. & Horvitz, H.R. The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation. Proc. Natl. Acad. Sci. USA 94, 3414-3419 (1997 (Pubitemid 27157326)
22. Middelkoop, T.C. et al. The thrombospondin repeat containing protein MIG-21 controls a left-right asymmetric Wnt signaling response in migrating C. elegans neuroblasts. Dev. Biol. 361, 338-348 (2012
23. Chronis, N., Zimmer, M. & Bargmann, C.I. Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans. Nat. Methods 4, 727-731 (2007 (Pubitemid 47338167)
24. Gilleland, C.L., Rohde, C.B., Zeng, F. & Yanik, M.F Microfluidic immobilization of physiologically active Caenorhabditis elegans. Nat. Protoc. 5, 1888-1902 (2010
25. Stiernagle, T. Maintenance of C. elegans. in WormBook, 1-11 (ed. The C. elegans Research Community) (2006
26. Cordes, S., Frank, C.A. & Garriga, G. The C. elegans MELK ortholog PIG-1 regulates cell size asymmetry and daughter cell fate in asymmetric neuroblast divisions. Development 133, 2747-2756 (2006 (Pubitemid 44177894)
27. Shakir, M.A., Gill, J.S. & Lundquist, E.A. Interactions of UNC-34 enabled with Rac GTPases and the NIK kinase MIG-15 in Caenorhabditis elegans axon pathfinding and neuronal migration. Genetics 172, 893-913 (2006 (Pubitemid 43345427)
28. Chapman, J.O., Li, H. & Lundquist, E.A. The MIG-15 NIK kinase acts cell-autonomously in neuroblast polarization and migration in C. elegans. Dev. Biol. 324, 245-257 (2008 (Pubitemid 50317701)
29. Honigberg, L. & Kenyon, C. Establishment of left/right asymmetry in neuroblast migration by UNC-40/DCC, UNC-73/Trio and DPY-19 proteins in C. elegans. Development 127, 4655-4668 (2000
30. Wolf, F.W., Hung, M.S., Wightman, B., Way, J. & Garriga, G. vab-8 is a key regulator of posteriorly directed migrations in C. elegans and encodes a novel protein with kinesin motor similarity. Neuron 20, 655-666 (1998 (Pubitemid 28199046)
31. Zipkin, I.D., Kindt, R.M. & Kenyon, C.J. Role of a new Rho family member in cell migration and axon guidance in C. elegans. Cell 90, 883-894 (1997 (Pubitemid 27381627)
32. Baum, P.D. & Garriga, G. Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin mutants. Neuron 19, 51-62 (1997 (Pubitemid 27332993)
33. Silhankova, M. & Korswagen, H.C. Migration of neuronal cells along the anterior-posterior body axis of C. elegans: Wnts are in control. Curr. Opin. Genet. Dev. 17, 320-325 (2007 (Pubitemid 47238608)
34. Kenyon, C. A gene involved in the development of the posterior body region of C. elegans. Cell 46, 477-487 (1986
35. Whangbo, J. & Kenyon, C. A Wnt signaling system that specifies two patterns of cell migration in C. elegans. Mol. Cell 4, 851-858 (1999
36. Pan, C.L. et al. Multiple Wnts and frizzled receptors regulate anteriorly directed cell and growth cone migrations in Caenorhabditis elegans. Dev. Cell 10, 367-377 (2006
37. Kim, S., Ren, X.C., Fox, E. & Wadsworth, W.G. SDQR migrations in Caenorhabditis elegans are controlled by multiple guidance cues and changing responses to netrin UNC-6. Development 126, 3881-3890 (1999). (Pubitemid 29440485)