Isolation, growth, and characterization of human renal epithelial cells using traditional and 3D methods

Methods in Molecular Biology

Volumn 945, Issue , 2013, Pages 329-345

  Gildea, John J ^a   McGrath, Helen E ^a   Van Sciver, Robert E ^a   Wang, Dora Bigler ^a   Felder, Robin A ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

3D cell culture; Human renal epithelial cells; Immortalization; Proximal tubule

Indexed keywords

TELOMERASE; VIRUS T ANTIGEN;

ADENOVIRUS; ARTICLE; CELL PROLIFERATION; CELL SEPARATION; CELL STRAIN HEK293; CRYOPRESERVATION; CULTURE TECHNIQUE; CYTOLOGY; EPITHELIUM CELL; GENETIC TRANSDUCTION; GENETICS; HUMAN; KIDNEY PROXIMAL TUBULE; LENTIVIRINAE; METABOLISM; METHODOLOGY;

ADENOVIRIDAE; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SEPARATION; CRYOPRESERVATION; EPITHELIAL CELLS; HEK293 CELLS; HUMANS; KIDNEY TUBULES, PROXIMAL; LENTIVIRUS; TELOMERASE; TRANSDUCTION, GENETIC;

EID: 84870532922     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-125-7_20     Document Type: Article

References (13)

1. Baer PC, Bereiter-Hahn J, Schubert R, Geiger H (2006) Differentiation status of human renal proximal and distal tubular epithelial cells in vitro: differential expression of characteristic markers. Cells Tissues Organs 184:16-22
2. Wilson PD, Dillingham MA, Breckon R, Anderson RJ (1985) De fined human renal tubular epithelia in culture: growth, characterization, and hormonal response. Am J Physiol 248:F436-F443
3. Detrisac CJ, Sens MA, Garvin AJ, Spicer SS, Sens DA (1984) Tissue culture of human kidney epithelial cells of proximal tubule origin. Kidney Int 25:383-390
4. Racusen LC, Monteil C, Sgrignoli A, Lucskay M, Marouillat S, Rhim JG, Morin JP (1997) Cell lines with extended in vitro growth potential from human renal proximal tubule: characterization, response to inducers, and comparison with established cell lines. J Lab Clin Med 129:318-329
5. Ryan MJ, Johnson G, Kirk J, Fuerstenberg SM, Zager RA, Torok-Storb B (1994) HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney. Kidney Int 45:48-57
6. Racusen LC, Wilson PD, Hartz PA, Fivush BA, Burrow CR (1995) Renal proximal tubular epithelium from patients with nephropathic cystinosis: immortalized cell lines as in vitro model systems. Kidney Int 48:536-543
7. Justice BA, Badr NA, Felder RA (2009) 3D cell culture opens new dimensions in cell-based assays. Drug Discov Today 14:102-107
8. Taub M, Sato G (1980) Growth offunctional primary cultures of kidney epithelial cells in de fined medium. J Cell Physiol 105: 369-378
9. Kowolik CM, Liang S, Yu Y, Yee JK (2004) Cre-mediated reversible immortalization of human renal proximal tubular epithelial cells. Oncogene 23:5950-5957
10. Loghman-Adham M, Nauli SM, Soto CE, Kariuki B, Zhou J (2003) Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts. Am J Physiol Renal Physiol 285:F397-F412
11. Lei KJ, Gluzman Y, Pan CJ, Chou JY (1992) Immortalization of virus-free human placental cells that express tissue-speci fic functions. Mol Endocrinol 6:703-712
12. Orosz DE, Woost PG, Kolb RJ, Finesilver MB, Jin W, Frisa PS, Choo CK, Yau CF, Chan KW, Resnick MI, Douglas JG, Edwards JC, Jacobberger JW, Hopfer U (2004) Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells. In Vitro Cell Dev Biol Anim 40: 22-34
13. Wieser M, Stadler G, Jennings P, Streubel B, Pfaller W, Ambros P, Riedl C, Katinger H, Grillari J, Grillari-Voglauer R (2008) hTERT alone immortalizes epithelial cells of renal proximal tubules without changing their functional characteristics. Am J Physiol Renal Physiol 295:F1365-F1375