Blow fly Lucilia sericata nuclease digests DNA associated with wound slough/eschar and with Pseudomonas aeruginosa biofilm

Medical and Veterinary Entomology

Volumn 26, Issue 4, 2012, Pages 432-439

  Brown, A ^a   Horobin, A ^a   Blount, D G ^a   Hill, P J ^a   English, J ^b   Rich, A ^b   Williams, P M ^a   Pritchard, D I ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b QUEEN S MEDICAL CENTRE   (United Kingdom)

Author keywords

Biofilm; Debridement; DNAse; Lucilia sericata; Pseudomonas aeruginosa; Wound

Indexed keywords

DEOXYRIBONUCLEASE; DNA; INSECT PROTEIN; METHYL GREEN;

BACTERIUM; BIOFILM; ENZYME ACTIVITY; FLY; LARVA; MITOCHONDRIAL DNA; SECRETION; VIRULENCE; WOUNDING;

ANIMAL; ARTICLE; BIOFILM; CHEMISTRY; DIPTERA; INJURY; LARVA; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS AERUGINOSA; WOUND HEALING;

ANIMALS; BIOFILMS; DEOXYRIBONUCLEASES; DIPTERA; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INSECT PROTEINS; LARVA; METHYL GREEN; PSEUDOMONAS AERUGINOSA; WOUND HEALING; WOUNDS AND INJURIES;

BACTERIA (MICROORGANISMS); CALLIPHORIDAE; DIPTERA; LUCILIA SERICATA; PSEUDOMONAS AERUGINOSA;

EID: 84870390839     PISSN: 0269283X     EISSN: 13652915     Source Type: Journal    
DOI: 10.1111/j.1365-2915.2012.01029.x     Document Type: Article

References (34)

1. Alexander, L.A., Korber, A., Grabbe, S. & Dissemond, J. (2007) Influence of pH on wound healing: a new perspective for wound-therapy? Archives of Dermatological Research, 298, 413-420.
2. Allesen-Holm, M., Barken, K.B., Yang, L. et al. (2006) A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Molecular Microbiology, 59, 1114-1128.
3. Andersen, A.S., Joergensen, B., Bjarnsholt, T., Johansen, H., Karlsmark, T., Givskov, M. & Krogfelt, K.M. (2010) Quorum-sensing-regulated virulence factors in Pseudomonas aeruginosa are toxic to Lucilia sericata maggots. Microbiology, 146, 400-407.
4. Arimatsu, Y., Kotani, E., Sugimura, Y. & Furusawa, T. (2007) Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. Insect Biochemistry and Molecular Biology, 37, 176-183.
5. Bennett, G., Dealey, C. & Posnett, J. (2004) The cost of pressure ulcers in the U.K. Age and Ageing, 33, 230-235.
6. Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding. Analytical Biochemistry, 72, 248-254.
7. Calvo, E. & Ribiero, J.M.C. (2006) A novel secreted endonuclease from Culex quinquefasciatus salivary glands. Journal of Experimental Biology, 209, 2651-2659.
8. Chambers, L., Woodrow, S., Brown, A.P. et al. (2003) Degradation of extracellular matrix components by defined proteinases from the greenbottle larva Lucilia sericata used for the clinical debridement of non-healing wounds. British Journal of Dermatology, 148, 14-23.
9. Church, J.C. (2001) Larval intervention in the chronic wound. European Wound Management Association, 1, 10-13.
10. Dass, C.L., Walsh, M.F., Seo, S., Shiratsuchi, H., Craig, D.H. & Basson, M.D. (2009) Irrigant divalent cation concentrations influence bacterial adhesion. Journal of Surgical Research, 156, 57-63.
11. Davies, C.E., Hill, K.E., Wilson, M.J., Stephens, P., Hill, C.M., Harding, K.G. & Thomas, D.W. (2004) Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and non-healing chronic venous leg ulcers. Journal of Clinical Microbiology, 42, 3549-3557.
12. Dumville, J.C., Worthy, G., Bland, J.M. et al. (2009) Larval therapy for leg ulcers (VenUS II): randomized controlled trial. British Medical Journal, 338, 1047-1054.
13. Fazli, M., Bjarnsholt, T., Kirketerp-Møller, K. et al. (2009) Non-random distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds. Journal of Clinical Microbiology, 47, 4084-4089.
14. Flemming, H.-C. & Wingender, J. (2010) The biofilm matrix. Nature Reviews Microbiology, 8, 623-633.
15. Grzesiak, J.J. & Pierschbacher, M.D. (1995) Shifts in the concentration of magnesium and calcium in early porcine and rat wound fluids activate the cell migratory response. Journal of Clinical Investigation, 95, 227-233.
16. Harris, L.G., Bexfield, A., Nigam, Y., Rohde, H., Ratcliffe, N.A. & Mack, D. (2009) Disruption of Staphylococcus epidermidis biofilms by medicinal maggot Lucilia sericata excretions/secretions. International Journal of Artificial Organs, 32, 555-564.
17. Harrison-Balestra, C., Cazzaniga, A.L., Davis, S.C. & Mertz, P.M. (2003) Wound-isolated Pseudomonas aeruginosa grows a biofilm in vitro within 10 hours and is visualized by light microscopy. Dermatologic Surgery, 29, 631-635.
18. Li, C., Hwa, K. & Englund, P.T. (1995) A DNAse from the trypanosomatid Crithidia fasciculata. Nucleic Acids Research, 23, 4426-4433.
19. Locke, I.C. & Carpenter, B.G. (2002) Ion-dependency of the streptococcal deoxyribonuclease, 'streptodornase', an active constituent of the medicament Varidase®. Enzyme and Microbial Technology, 31, 482-489.
20. Locke, I.C., Cox, S.F. & Carpenter, B.G. (1997) Purification of a streptococcal deoxyribonuclease by affinity chromatography based on a DNA-cellulose matrix. Journal of Chromatography A, 788, 75-80.
21. Maklebust, J. (1996) Using wound care products to promote a healing environment. Critical Care Nursing Clinics of North America, 8, 141-158.
22. Nene, V., Wortman, J.R., Lawson, D. et al. (2007) Genome sequence of Aedes aegypti, a major arbovirus vector. Science, 316, 1718-1723.
23. van der Plas, M.J.A., Jukema, G.N., Wai, S.-W. et al. (2008) Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Journal of Antimicrobial Chemotherapy, 61, 117-122.
24. van der Plas, M.J.A., Dambrot, C., Dogterom-Ballering, H.C.M., Kruithof, S., van Dissel, J.T. & Nibbering, P.H. (2010) Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom. Journal of Antimicrobial Chemotherapy, 65, 917-923.
25. Rutter, P.M., Carpenter, B., Hill, S.S. & Locke, I.C. (2000) Varidase: the science behind the medicament. Journal of Wound Care, 9, 223-226.
26. Sherman, R.A., Hall, M.J.R. & Thomas, S. (2000) Medicinal maggots: an ancient remedy for some contemporary afflictions. Annual Review of Entomology, 45, 55-81.
27. Shuai, K., Das Gupta, C.K., Scott Hawley, R., Chase, K.W., Stone, K.L. & Williams, K.R. (1992) Purification and characterization of an endo-exonuclease from adult flies of Drosophila melanogaster. Nucleic Acids Research, 20, 1379-1385.
28. Sinicropi, D., Baker, D.L., Prince, W.S., Shiffer, K. & Shak, S. (1994) Colorimetric determination of DNAse I activity with a DNA-methyl green substrate. Analytical Biochemistry, 222, 351-358.
29. Steed, D.L. (2004) Debridement. American Journal of Surgery, 187 (Suppl.), 71-74.
30. Sun, Y., Dowd, S.E., Smith, E., Rhoads, D.D. & Wolcott, R.D. (2008) In vitro multispecies Lubbock chronic wound biofilm model. Wound Repair and Regeneration, 16, 805-813.
31. Telford, G., Brown, A.P., Seabra, R.A.M., Horobin, A.J., Rich, A., English, J.S.C. & Pritchard, D.I. (2010) Degradation of eschar from venous leg ulcers using a recombinant chymotrypsin from Lucilia sericata. British Journal of Dermatology, 163, 523-531.
32. Telford, G., Brown, A.P., Rich, A., English, J.S.C. & Pritchard, D.I. (2011) Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors. British Journal of Dermatology, 164, 192-196.
33. Tetz, G.V., Artemenko, N.K. & Tetz, V.V. (2009) Effect of DNAse and antibiotics on biofilm characteristics. Antimicrobial Agents and Chemotherapy, 53, 1204-1209.
34. Whitchurch, C.B., Tolker-Nielsen, T., Ragas, P.C. & Mattick, J.S. (2002) Extracellular DNA required for bacterial biofilm formation. Science, 295, 1487.