Identification of the SGR6065 gene product as a sesquiterpene cyclase involved in (+)-epicubenol biosynthesis in Streptomyces griseus

Journal of Antibiotics

Volumn 65, Issue 11, 2012, Pages 551-558

  Nakano, Chiaki ^a   Tezuka, Takeaki ^a   Horinouchi, Sueharu ^a   Ohnishi, Yasuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

(+) epicubenol; genome mining; natural product; sesquiterpene cyclase; Streptomyces griseus

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL ENZYME; EPICUBENOL; FACTOR A; FARNESYL DIPHOSPHATE; HISTIDINE; PLASMID DNA; SESQUITERPENE DERIVATIVE; STREPTOMYCES GRISEUS EPICUBENOL SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL LYSATE; CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG ISOLATION; DRUG STRUCTURE; ESCHERICHIA COLI; GENE DELETION; GENE IDENTIFICATION; GENE MUTATION; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; HETERONUCLEAR MULTIPLE BOND CORRELATION; HETERONUCLEAR MULTIPLE QUANTUM COHERENCE; IN VITRO STUDY; IN VIVO STUDY; KNOCKOUT GENE; MOLECULAR CLONING; NONHUMAN; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; STOP CODON; STREPTOMYCES GRISEUS; STREPTOMYCES LIVIDANS; WILD TYPE;

BACTERIAL PROTEINS; BASE SEQUENCE; CARBON-CARBON LYASES; DNA, BACTERIAL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; ISOMERASES; KINETICS; MUTATION; RECOMBINANT PROTEINS; SESQUITERPENES; STEREOISOMERISM; STREPTOMYCES GRISEUS; TRANSCRIPTOME;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HETEROSCYPHUS PLANUS; POSIBACTERIA; STREPTOMYCES; STREPTOMYCES GRISEUS; STREPTOMYCES LIVIDANS; STREPTOMYCES SP.;

EID: 84870323745     PISSN: 00218820     EISSN: 18811469     Source Type: Journal    
DOI: 10.1038/ja.2012.68     Document Type: Article

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