Enumeration of viable Escherichia coli by real-time PCR with propidium monoazide

Water Science and Technology

Volumn 66, Issue 10, 2012, Pages 2065-2073

  Yokomachi, N ^a   Yaguchi, J ^a  

^a HACHINOHE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

DNA; Escherichia coli; Propidium monoazide; Real time PCR; Viable but non culturable

Indexed keywords

CULTURABLE; DEAD CELLS; DNA-BINDING; E. COLI; ESCHERICHIA COLI CELLS; MEMBRANE DAMAGE; MICROSCOPIC OBSERVATIONS; MOST PROBABLE NUMBER METHODS; PROPIDIUM; REAL-TIME PCR; REAL-TIME POLYMERASE CHAIN REACTION; VIABLE BUT NON-CULTURABLE; VIABLE CELLS; WASTEWATER TREATMENT PLANTS;

CELLS; CHLORINE; CYTOLOGY; DNA; ESCHERICHIA COLI; SEWAGE PUMPING PLANTS; WATER TREATMENT PLANTS;

POLYMERASE CHAIN REACTION;

CHLORINE; DYE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; DNA; DYE; MEMBRANE; POLYMERASE CHAIN REACTION; REAL TIME; WASTE TREATMENT;

ARTICLE; BACTERIAL CELL; BACTERIAL VIABILITY; CELL DEATH; CELL VIABILITY; DNA EXTRACTION; ESCHERICHIA COLI; FECAL COLIFORM; HEAT TREATMENT; LIMIT OF DETECTION; MEMBRANE DAMAGE; MOST PROBABLE NUMBER METHOD; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SEWAGE TREATMENT PLANT; WASTE WATER MANAGEMENT; WASTE WATER TREATMENT PLANT;

AZIDES; DNA, BACTERIAL; ESCHERICHIA COLI; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; STAINING AND LABELING; WASTE DISPOSAL, FLUID; WATER MICROBIOLOGY;

ESCHERICHIA COLI;

EID: 84870313927     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2012.370     Document Type: Article

References (22)

1. Bej, A. K., DiCesare, J. L., Haff, L. & Atlas, R. M. 1991 Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. Appl. Environ. Microbiol. 57, 1013-1017.
2. Byrd, J. J., Xu, H. & Colwell, R. R. 1991 Viable but nonculturable bacteria in drinking water. Appl. Environ. Microbiol. 57, 875-878.
3. Colwell, R. R., Brayton, P., Grimes, D. J., Roszak, D. B., Huq, S. A. & Palmer, L. M. 1985 Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Bio/Technology 3, 817-820.
4. Coutard, F., Pommepuy, M., Loaec, S. & Hervio-Heath, D. 2005 mRNA detection by reverse transcription-PCR for monitoring viability and potential virulence in a pathogenic strain of Vibrio parahaemolyticus in viable but nonculturable state. J. Appl. Microbiol. 98, 951-961.
5. Del Mar Lleo, M., Pierobon, S., Carla Tafi, M., Signoretto, C. & Canepari, P. 2000 mRNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm. Appl. Environ. Microbiol. 66, 4564-4567.
6. Frahm, E. & Obst, U. 2003 Application of the fluorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples. J. Microbiol. Methods 52, 123-131.
7. Flekna, G., Stefanic, P., Wagner, M., Smulders, F. J. M., Mozina, S. S. & Hein, L. 2007 Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR. Res. Microbiol. 158, 405-412.
8. He, J. & Jiang, S. 2005 Quantification of Enterococci and human adenoviruses in environmental samples by real-time PCR. Appl. Environ. Microbiol. 71, 2250-2255.
9. Inoue, D., Tsutsui, Y., Yamazaki, Y., Sei, K., Soda, S., Fujita, M. & Ike, M. 2008 Application of real-time polymerase chain reaction (PCR) coupled with ethidium monoazide treatment for selective quantification of viable bacteria in aquatic environment. Wat. Sci. Technol. 58, 1107-1112.
10. Josephson, K. L., Gerba, C. P. & Pepper, I. L. 1993 Polymerase chain reaction detection of nonviable bacterial pathogens. Appl. Environ. Microbiol. 59, 3513-3515.
11. JSWA 1997 Japanese Standard Methods for the Examination of Wastewater. Japan Sewage Works Association, Tokyo, Japan. (in Japanese).
12. Kahla-Nakbi, A. B., Besbes, A., Chaieb, K., Rouabhia, M. & Bakhrouf, A. 2007 Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells. Marine Environ. Res. 64, 469-478.
13. Nocker, A., Cheung, C. Y. & Camper, A. K. 2006 Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Methods 67, 310-320.
14. Nogva, H. K., Dromtorp, S. M., Nissen, H. & Rudi, K. 2003 Ethidium monoazide for DNA-based differentiation of viable and dead bacteria. BioTechniques 34, 804-813.
15. Pan, Y. & Breidt Jr., F. 2007 Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl. Environ. Microbiol. 73, 8028-8031.
16. Rahman, I., Shahamat, M., Chowdhury, M. A. R. & Colwell, R. R. 1996 Potential virulence of viable but nonculturable Shigella dysenteriae Type 1. Appl. Environ. Microbiol. 62, 115-120.
17. Rudi, K., Moen, B., Dromtorp, S. M. & Holck, A. L. 2005 Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Appl. Environ. Microbiol. 71, 1018-1024.
18. Roszak, D. B. & Colwell, R. R. 1987 Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51, 365-379.
19. Sawaya, K., Kaneko, N., Fukushi, K. & Yaguchi, J. 2008 Behaviors of physiologically active bacteria in water environment and chlorine disinfection.Wat. Sci. Technol. 58, 1343-1348.
20. Sheridan, G. E. C., Szabo, E. A. & Mackey, B. M. 1999 Effect of post-treatment holding conditions on detection of tufa mRNA in ethanol-treated Escherichia coli: implications for RT-PCR-based indirect viability tests. Lett. Appl. Microbiol. 29, 375-379.
21. Varma, M., Field, R., Stinson, M., Rukovets, B., Wymer, L. & Haugland, R. 2009 Quantitative real-time PCR analysis of total and propidium monoazide-resistant fecal indicator bacteria in wastewater. Wat. Res. 43, 4790-4801.
22. Zimbro, M. J., Power, D. A., Miller, S. M., Wilson, G. E. & Johnson, J. A. 2009 Difco & BBL Manual: Manual of Microbiological Culture Media. Becton, Dickinson &Company, Sparks, MD, USA, pp. 201-202.