Aralar mRNA and protein levels in neurons and astrocytes freshly isolated from young and adult mouse brain and in maturing cultured astrocytes

Neurochemistry International

Volumn 61, Issue 8, 2012, Pages 1325-1332

  Li, Baoman ^a   Hertz, Leif ^a   Peng, Liang ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

Aralar; Astrocyte; Astrocyte culture; Glutamate; Neuron; Protein expression

Indexed keywords

ARALAR; ASPARTIC ACID; GLUTAMIC ACID; MESSENGER RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; ASTROCYTE; BRAIN FUNCTION; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL MATURATION; CELL SELECTION; FEMALE; MALE; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AGING; ANIMALS; ASPARTIC ACID; ASTROCYTES; BRAIN; CELL AGING; CELL SEPARATION; CELLS, CULTURED; CYTOPLASM; ENERGY METABOLISM; FEMALE; GLUTAMIC ACID; LACTIC ACID; MALE; MICE; MICE, TRANSGENIC; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; NAD; NERVE TISSUE PROTEINS; NEURONS; OXIDATION-REDUCTION; PRIMARY CELL CULTURE; PYRUVIC ACID; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 84870301157     PISSN: 01970186     EISSN: 18729754     Source Type: Journal    
DOI: 10.1016/j.neuint.2012.09.009     Document Type: Article

References (59)

1. A. Almeida, K.J. Brooks, I. Sammut, J. Keelan, G.P. Davey, J.B. Clark, and T.E. Bates Postnatal development of the complexes of the electron transport chain in synaptic mitochondria from rat brain Dev. Neurosci. 17 1995 212 218
2. A.I. Amaral, A.P. Teixeira, B.I. Håkonsen, U. Sonnewald, and P.M. Alves A comprehensive metabolic profile of cultured astrocytes using isotopic transient metabolic flux analysis and C-labeled glucose Front Neuroenerg. 3 2011 5
3. T.E. Bates, S.J. Heales, S.E. Davies, P. Boakye, and J.B. Clark Effects of 1-methyl-4-phenylpyridinium on isolated rat brain mitochondria: evidence for a primary involvement of energy depletion J. Neurochem. 63 1994 640 648
4. D.E. Bauer, J.G. Jackson, E.N. Genda, M.M. Montoya, M. Yudkoff, and M.B. Robinson The glutamate transporter, GLAST, participates in a macromolecular complex that supports glutamate metabolism Neurochem. Int. 61 2012 566 574
5. D.A. Berkich, M.S. Ola, J. Cole, A.J. Sweatt, S.M. Hutson, and K.F. LaNoue Mitochondrial transport proteins of the brain J. Neurosci. Res. 85 2007 3367 3377
6. S. Blüml, A. Moreno-Torres, F. Shic, C.H. Nguy, and B.D. Ross Tricarboxylic acid cycle of glia in the in vivo human brain NMR Biomed. 15 2002 1 5
7. 13C nuclear magnetic resonance spectroscopy J. Neurosci. 30 2010 13983 13991
8. J.D. Cahoy, B. Emery, A. Kaushal, L.C. Foo, J.L. Zamanian, K.S. Christopherson, Y. Xing, J.L. Lubischer, P.A. Krieg, S.A. Krupenko, W.J. Thompson, and B.A. Barres A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function J. Neurosci. 28 2008 264 278
9. G.M. Chowdhury, A.B. Patel, G.F. Mason, D.L. Rothman, and K.L. Behar Glutamatergic and GABAergic neurotransmitter cycling and energy metabolism in rat cerebral cortex during postnatal development J. Cereb. Blood Flow Metab. 27 2007 1895 1907
10. L. Contreras, A. Urbieta, K. Kobayashi, T. Saheki, and J. Satrústegui Low levels of citrin (SLC25A13) expression in adult mouse brain restricted to neuronal clusters J. Neurosci. Res. 88 2010 1009 1016
11. 13C NMR studies of metabolic compartmentation in the adult mammalian brain NMR Biomed. 12 1999 451 462
12. N.C. Danbolt Glutamate uptake Prog. Neurobiol. 65 2001 1 105
13. D.K. Deelchand, C. Nelson, A.A. Shestov, K. Uǧurbil, and P.G. Henry Simultaneous measurement of neuronal and glial metabolism in rat brain in vivo using co-infusion of [1,6-13C2]glucose and [1,2-13C2]acetate J. Magn. Reson. 196 2009 157 163
14. A. del Arco, J. Morcillo, J.R. Martínez-Morales, C. Galián, V. Martos, P. Bovolenta, and J. Satrústegui Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues Eur. J. Biochem. 269 2002 3313 3320
15. M. Erecińska, D. Pleasure, D. Nelson, I. Nissim, and M. Yudkoff Cerebral aspartate utilization: near-equilibrium relationships in aspartate aminotransferase reaction J Neurochem. 60 1993 1696 1706
16. S. Fedoroff, W.A. McAuley, J.D. Houle, and R.M. Devon Astrocyte cell lineage. V. Similarity of astrocytes that form in the presence of dBcAMP in cultures to reactive astrocytes in vivo J. Neurosci. Res. 12 1984 14 27
17. S.M. Fitzpatrick, A.J. Cooper, and L. Hertz Effects of ammonia and beta-methylene-dl-aspartate on the oxidation of glucose and pyruvate by neurons and astrocytes in primary culture J Neurochem. 51 1988 Oct 1197 1203
18. F. Fonnum The distribution of glutamate decarboxylase and aspartate transaminase in subcellular fractions of rat and guinea-pig brain Biochem. J. 106 1968 401 412
19. J.M. Fritschy Is my antibody-staining specific? How to deal with pitfalls of immunohistochemistry Eur. J. Neurosci. 28 2008 2365 2370
20. 1/2 and HMIT to myo-inositol uptake at different concentrations and pH Neurochem. Int. 61 2012 187 194
21. L. Hertz Dibutyryl cyclic AMP treatment of astrocytes in primary cultures as a substitute for normal morphogenic and 'functiogenic' transmitter signals A. Privat, E. Giacobini, P. Timiras, A. Vernadakis, Molecular Aspects of Development and Aging in the Nervous System 1990 Plenum N.Y. 227 243
22. L. Hertz Metabolic interactions between neurons and astrocytes S. Fedoroff, R. Doucette, B.H.J. Juurlink, Biology and Pathology of Astrocyte-Neuron Interactions 1993 Plenum New. Y. 1 13
23. L. Hertz Brain glutamine synthesis requires neuronal aspartate: a commentary J. Cereb. Blood Flow Metab. 31 2011 384 387
24. L. Hertz Astrocytic energy metabolism and glutamate formation-relevance for 13C-NMR spectroscopy and importance of cytosolic/mitochondrial trafficking Magn. Reson. Imaging 29 2011 1319 1329
25. L. Hertz Isotope-based quantitation of uptake, release, and metabolism of glutamate and glucose in cultured astrocytes Methods Mol. Biol. 814 2012 305 323
26. L. Hertz, E. Bock, and A. Schousboe GFA content, glutamate uptake and activity of glutamate metabolizing enzymes in differentiating mouse astrocytes in primary cultures Dev. Neurosci. 1 1978 226 238
27. L. Hertz, and G.A. Dienel Energy metabolism in the brain Int. Rev. Neurobiol. 51 2002 1 102
28. L. Hertz, L. Peng, and G.A. Dienel Energy metabolism in astrocytes: high rate of oxidative metabolism and spatiotemporal dependence on glycolysis/glycogenolysis J. Cereb. Blood Flow Metab. 27 2007 219 249
29. L. Hertz, L. Peng, and J.C. Lai Functional studies in cultured astrocytes Methods 16 1998 293 310
30. D. Holtzman, J. Olson, S. Zamvil, and H. Nguyen J. Neurochem. 39 1982 274 276
31. Y. Horio, T. Tanaka, M. Taketoshi, T. Uno, and H. Wada Rat cytosolic aspartate aminotransferase: regulation of its mRNA and contribution to gluconeogenesis J. Biochem. 103 1988 805 808
32. H.K. Kimelberg Functions of mature mammalian astrocytes: a current view Neuroscientist 16 2010 79 106
33. J.M. Land, R.F. Booth, R. Berger, and J.B. Clark Development of mitochondrial energy metabolism in rat brain Biochem. J. 164 1977 339 348
34. K.F. LaNoue, V. Carson, D.A. Berkich, and S.M. Hutson Mitochondrial/Cytosolic Interactions via Metabolite Shuttles and Transporters, in Handbook of Neurochemistry and Molecular Neurobiology A. Lajtha, G.E. Gibson, G.A. Dienel, 2007 Springer-Verlag Berlin 5616 5689 Vol. 2
35. B. Lanz, K. Uffmann, T. Wyss, M. Weber, B. Buck, A. Buck, and R. Gruetter A two-compartment mathematical model of neuroglial metabolism using [1-11C] acetate J. Cereb. Blood Flow Metab. 32 2012 548 559
36. 13C nuclear magnetic resonance spectroscopy: elucidation of the dominant pathway for neurotransmitter glutamate repletion and measurement of astrocytic oxidative metabolism J. Neurosci. 22 2002 1523 1531
37. S.F. Leong, and J.B. Clark Regional enzyme development in rat brain. Enzymes associated with glucose utilization Biochem. J. 218 1984 131 138
38. Z. Lodin, J. Faltin, and P. Korínková The effect of dibutyryl cyclic AMP on cultivated glial cells from corpus callosum of 30-day-old rats Physiol. Bohemoslov. 28 1979 105 111
39. D. Lovatt, U. Sonnewald, H.S. Waagepetersen, A. Schousboe, W. He, J.H. Lin, X. Han, T. Takano, S. Wang, F.J. Sim, S.A. Goldman, and M. Nedergaard The transcriptome and metabolic gene signature of protoplasmic astrocytes in the adult murine cortex J. Neurosci. 27 2007 12255 12266
40. + clearance and cell swelling: key roles for cotransporters and pumps. Neurochem. Res. Feb 26. [Epub ahead of print].
41. P. Malik, M.C. McKenna, and J.T. Tildon Regulation of malate dehydrogenases from neonatal, adolescent, and mature rat brain Neurochem. Res. 18 1993 247 257
42. M.C. McKenna, J.H. Stevenson, X. Huang, and I.B. Hopkins Differential distribution of the enzymes glutamate dehydrogenase and aspartate aminotransferase in cortical synaptic mitochondria contributes to metabolic compartmentation in cortical synaptic terminals Neurochem. Int. 37 2000 229 241
43. E. Meier, L. Hertz, and A. Schousboe Neurotransmitters as developmental signals Neurochem. Int. 19 1991 1 15
44. G. Moonen, and M. Sensenbrenner Effects of dibutyryl cyclic AMP on cultured brain cells from chick embryos of different ages Experientia 32 1976 40 42
45. N.H. Nguyen, A. Bråthe, and B. Hassel Neuronal uptake and metabolism of glycerol and the neuronal expression of mitochondrial glycerol-3-dehydrogenase J. Neurochem. 85 2003 831 842
46. K. Nishino, and T.S. Nowak Jr. Time course and cellular distribution of hsp27 and hsp72 stress protein expression in a quantitative gerbil model of ischemic injury and tolerance. thresholds for hsp72 induction and hilar lesioning in the context of ischemic preconditioning J. Cereb. Blood Flow Metab. 24 2004 167 178
47. B. Pardo, T.B. Rodrigues, L. Contreras, M. Garzón, I. Llorente-Folch, K. Kobayashi, T. Saheki, S. Cerdan, and J. Satrústegui Brain glutamine synthesis requires neuronal-born aspartate as amino donor for glial glutamate formation J. Cereb. Blood Flow Metab. 31 2011 90 101
48. 13C]-NMR J. Cereb. Blood Flow Metab. 30 2010 1200 1213
49. A.J. Patel, and R. Balázs Manifestation of metabolic compartmentation during the maturation of the rat brain J. Neurochem. 17 1970 955 971
50. A.J. Patel, A. Hunt, R.D. Gordon, and R. Balázs The activities in different neural cell types of certain enzymes associated with the metabolic compartmentation glutamate Brain Res. 256 1982 3 11
51. L.A. Peng, B.H. Juurlink, and L. Hertz Differences in transmitter release, morphology, and ischemia-induced cell injury between cerebellar granule cell cultures developing in the presence and in the absence of a depolarizing potassium concentration Dev. Brain Res. 63 1991 1 12
52. L.J. Pérez, H. Díaz de Arce, F. Cilloni, A. Salviato, S. Marciano, C.L. Perera, A. Salomoni, M.S. Beato, A. Romero, I. Capua, and G. Cattoli An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus Mol. Cell Probes. 26 2012 137 145
53. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res. 29 2001 e45
54. 2+-regulated mitochondrial aspartate-glutamate carrier aralar1 in brain and prominent expression in the spinal cord Brain Res. Dev. Brain Res. 143 2003 33 46
55. A. Schousboe, G. Svenneby, and L. Hertz Uptake and metabolism of glutamate in astrocytes cultured from dissociated mouse brain hemispheres J. Neurochem. 29 1977 999 1005
56. P. Schubert, T. Morino, H. Miyazaki, T. Ogata, Y. Nakamura, C. Marchini, and S. Ferroni Cascading glia reactions: a common pathomechanism and its differentiated control by cyclic nucleotide signaling Ann. N. Y. Acad. Sci. 903 2000 24 33
57. G.G. Somjen, H. Kager, and W.J. Wadman Computer simulations of neuron-glia interactions mediated by ion flux J. Comput. Neurosci. 25 2008 349 365
58. F. Wandosell, P. Bovolenta, and M. Nieto-Sampedro Differences between reactive astrocytes and cultured astrocytes treated with di-butyryl-cyclic AMP J. Neuropathol. Exp. Neurol. 52 1993 205 215
59. 2+-dependent uptake of extracellular K+. Sci. Signal 5, ra26.