Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa

Plant Molecular Biology

Volumn 80, Issue 6, 2012, Pages 621-629

  Ueno, Hiroki ^a   Matsumoto, Etsuo ^b   Aruga, Daisuke ^a   Kitagawa, Satoshi ^a   Matsumura, Hideo ^a   Hayashida, Nobuaki ^a  

^a SHINSHU UNIVERSITY   (Japan)

^b Nagano Vegetable and Ornamental Crops Experiment Station   (Japan)

Author keywords

Brassicaceae; Chinese cabbage; Clubroot; CRa; Resistance gene; UV mutation

Indexed keywords

VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BRASSICA RAPA; CHROMOSOME MAP; GENETICS; MOLECULAR GENETICS; MUTAGENESIS; PARASITOLOGY; PATHOGENICITY; PLANT DISEASE; PLANT GENE; PLASMODIOPHORIDA; SEQUENCE HOMOLOGY; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; BRASSICA RAPA; CHROMOSOME MAPPING; GENES, PLANT; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLANT DISEASES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PLASMODIOPHORIDA; SEQUENCE HOMOLOGY, AMINO ACID;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BRASSICA RAPA; BRASSICA RAPA SUBSP. PEKINENSIS; BRASSICA RAPA SUBSP. RAPA; BRASSICACEAE; PLASMODIOPHORA; PLASMODIOPHORA BRASSICAE;

EID: 84870237815     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9971-5     Document Type: Article

References (44)

1. Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815.
2. Ayliffe MA, Frost DV, Finnegan EJ, Lawrence GJ, Anderson PA, Ellis JG (1999) Analysis of alternative transcripts of the flax L6 rust resistance gene. Plant J 17: 287-292.
3. Ballvora A, Ercolano MR, Weiss J, Meksem K, Bormann CA, Oberhagemann P, Salamini F, Gebhardt C (2002) The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant J 30: 361-371.
4. Bernoux M, Ellis JG, Dodds PN (2011) New insights in plant immunity signaling activation. Curr Opin Plant Biol 14: 512-518.
5. Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, Piao ZY, Soengas P, Han TH, King GJ, Barker GC, Hand P, Lydiate DJ, Batley J, Edwards D, Koo DH, Bang JW, Park BS, Lim YP (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115: 777-792.
6. DeYoung BJ, Innes RW (2006) Plant NBS-LRR proteins in pathogen sensing and host defense. Nat Immunol 7: 1243-1249.
7. Diederichsen E, Frauen M, Linders EGA, Hatakeyama K, Hirai M (2009) Status and perspectives of clubroot resistance breeding in crucifer crops. J Plant Growth Regul 28: 265-281.
8. Dinesh-Kumar SP, Baker BJ (2000) Alternatively spliced N resistance gene transcripts: their possible role in tobacco mosaic virus resistance. Proc Natl Acad Sci USA 97: 1908-1913.
9. Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349.
10. Frost D, Way H, Howles P, Luck J, Manners J, Hardham A, Finnegan J, Ellis J (2004) Tobacco transgenic for the flax rust resistance gene L expresses allele-specific activation of defense responses. Mol Plant Microbe Interact 17: 224-232.
11. Gassmann W (2008) Alternative splicing in plant defense. Curr Top Microbiol 326: 219-233.
12. Hayashida N, Takabatake Y, Nakazawa N, Aruga D, Nakanishi H, Taguchi G, SakaMoto K, Matsumoto E (2008) Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage, with intensive analysis of HC352b genes. J Jpn Soc Hortic Sci 77: 150-154.
13. Horiguchi G, Kodama H, Iba K (2001) Characterization of Arabidopsis mutants that are associated with altered C18 unsaturated fatty acid metabolism. Plant Sci 161: 1117-1123.
14. Howard RJ, Strelkov SE, Harding MW (2010) Clubroot of cruciferous crops-new perspectives on an old disease. Can J Plant Pathol 32: 43-57.
15. Jia Z, Cui Y, Li Y, Wang X, Du Y, Huang S (2010) Inducible positive mutant screening system to unveil the signaling pathway of late blight resistance. J Integr Plant Biol 52: 476-484.
16. Johnston JS, Pepper AE, Hall AE, Chen ZJ, Hodnett G, Drabek J, Lopez R, Price HJ (2005) Evolution of genome size in Brassicaceae. Ann Bot Lond 95: 229-235.
17. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12: 172-175.
18. Kou Y, Wang S (2010) Broad-spectrum and durability: understanding of quantitative disease resistance. Curr Opin Plant Biol 13: 181-185.
19. Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur J Plant Pathol 105: 327-332.
20. Leister D (2004) Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance genes. Trends Genet 20: 116-122.
21. Marathe R, Anandalakshmi R, Liu YL, Dinesh-Kumar SP (2002) The tobacco mosaic virus resistance gene, N. Mol Plant Pathol 3: 167-172.
22. Matsumoto E, Yasui C, Ohi M, Tsukada M (1998) Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis). Euphytica 104: 79-86.
23. Matsumoto E, Hayashida N, Sakamoto K, Ohi M (2005) Behavior of DNA markers linked to a clubroot resistance gene in segregating populations of Chinese cabbage (Brassica rapa spp. pekinensis). J Jpn Soc Hortic Sci 74: 367-373.
24. Matsumoto E, Ueno H, Aruga D, Sakamoto K, Hayashida N (2012) Accumulation of three clubroot resistance genes through marker-assisted selection in Chinese cabbage (Brassica rapa ssp. pekinensis). J Jpn Soc Hortic Sci 81: 184-190.
25. Matsumura H, Bin Nasir KH, Yoshida K, Ito A, Kahl G, Kruger DH, Terauchi R (2006) SuperSAGE array: the direct use of 26-base-pair transcript tags in oligonucleotide arrays. Nat Methods 3: 469-474.
26. Mun JH, Kwon SJ, Yang TJ, Kim HS, Choi BS, Baek S, Kim JS, Jin M, Kim JA, Lim MH, Lee SI, Kim HI, Kim H, Lim YP, Park BS (2008) The first generation of a BAC-based physical map of Brassica rapa. BMC Genomics 9: 280-291.
27. Mun JH, Yu HJ, Park S, Park BS (2009) Genome-wide identification of NBS-encoding resistance genes in Brassica rapa. Mol Genet Genomics 282: 617-631.
28. Naito K, Kusaba M, Shikazono N, Takano T, Tanaka A, Tanisaka T, Nishimura M (2005) Transmissible and nontransmissible mutations induced by irradiating Arabidopsis thaliana pollen with gamma-rays and carbon ions. Genetics 169: 881-889.
29. Nakanishi H, Suzuki K, Jouke T, Kodaira R, Taguchi G, Okazaki M, Hayashida N (2004) Collection and analysis of Arabidopsis color mutants. Endocytobiosis Cell Res 15: 328-338.
30. Nakanishi H, Nozue H, Suzuki K, Kaneko Y, Taguchi G, Hayashida N (2005) Characterization of the Arabidopsis thaliana mutant pcb2 which accumulates divinyl chlorophylls. Plant Cell Physiol 46: 467-473.
31. Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, Fujibe T, Matsumura H, Shenton M, Galam DC, Undan J, Ito A, Sone T, Terauchi R (2011) A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J 66: 467-479.
32. Piao ZY, Deng YQ, Choi SR, Park YJ, Lim YP (2004) SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet 108: 1458-1465.
33. Piao ZY, Ramchiary N, Lim YP (2009) Genetics of clubroot resistance in brassica species. J Plant Growth Regul 28: 252-264.
34. Rafiqi M, Bernoux M, Ellis JG, Dodds PN (2009) In the trenches of plant pathogen recognition: role of NB-LRR proteins. Semin Cell Dev Biol 20: 1017-1024.
35. Saito M, Kubo N, Matsumoto S, Suwabe K, Tsukada M, Hirai M (2006) Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa. Theor Appl Genet 114: 81-91.
36. Sakamoto K, Saito A, Hayashida N, Taguchi G, Matsumoto E (2008) Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Theor Appl Genet 117: 759-767.
37. Schmidt S, Lombardi M, Gardiner DM, Ayliffe M, Anderson PA (2007) The M flax rust resistance pre-mRNA is alternatively spliced and contains a complex upstream untranslated region. Theor Appl Genet 115: 373-382.
38. Schranz ME, Lysak MA, Mitchell-Olds T (2006) The ABC's of comparative genomics in the Brassicaceae: building blocks of crucifer genomes. Trends Plant Sci 11: 535-542.
39. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M (2003) Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor Appl Genet 107: 997-1002.
40. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S (2006) Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance. Genetics 173: 309-319.
41. Taguchi G, Ubukata T, Nozue H, Kobayashi Y, Takahi M, Yamamoto H, Hayashida N (2010) Malonylation is a key reaction in the metabolism of xenobiotic phenolic glucosides in Arabidopsis and tobacco. Plant J 63: 1031-1041.
42. Takabatake R, Seo SM, Mitsuhara I, Tsuda S, Ohashi Y (2006) Accumulation of the two transcripts of the N gene, conferring resistance to tobacco mosaic virus, is probably important for N gene-dependent hypersensitive cell death. Plant Cell Physiol 47: 254-261.
43. Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56: 624-626.
44. Zhang X-C, Gassmann W (2007) Alternative splicing and mRNA levels of the disease resistance gene RPS4 are induced during defense responses. Plant Physiol 145: 1577-1587.