Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Plant Molecular Biology

Volumn 80, Issue 6, 2012, Pages 631-646

  Qin, Xiaoqiong ^a   Zhang, Wenjun ^a   Dubcovsky, Jorge ^a   Tian, Li ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

hydroxylase; Carotenoid; Homeolog; Lutein; Provitamin A; Wheat

Indexed keywords

BETA CAROTENE HYDROXYLASE; BETA-CAROTENE HYDROXYLASE; CAROTENOID; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN;

ARTICLE; COMPARATIVE STUDY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; MOLECULAR CLONING; NUTRITIONAL VALUE; PHYLOGENY; PLANT GENE; POLYPLOIDY; SPECIES DIFFERENCE; WHEAT;

CAROTENOIDS; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; HUMANS; MIXED FUNCTION OXYGENASES; NUTRITIVE VALUE; PHYLOGENY; POLYPLOIDY; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; TRITICUM;

ESCHERICHIA COLI; TRITICUM AESTIVUM; TRITICUM TURGIDUM;

EID: 84870236520     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9972-4     Document Type: Article

References (43)

1. Applied Biosystems (2004) Guide to performing relative quantitation of gene expression using Real-Time quantitative PCR.
2. Bouvier F, Keller Y, d'Harlingue A, Camara B (1998) Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.). Biochim Biophys Acta 1391: 320-328.
3. Britton G (1995) UV/visible spectroscopy. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol. 1B: Spectroscopy. Birkhauser, Basel, Switzerland pp 13-62.
4. Britton G (2009) Vitamin A and vitamin A deficiency. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids. Volume 5: Nutrition and Health. Birkhäuser Verlag Basel.
5. Brown C, Kim T, Ganga Z, Haynes K, De Jong D, Jahn M, Paran I, De Jong W (2006) Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism. Am J Potato Res 83: 365-372.
6. Cunningham FJ, Gantt E (2007) A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Photosynth Res 92: 245-259.
7. Cunningham F, Pogson B, Sun Z, McDonald K, DellaPenna D, Gantt E (1996) Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8: 1613-1626.
8. Devos K, Dubcovsky J, Dvorak J, Chinoy C, Gale M (1995) Structural evolution of wheat chromosomes 4A, 5A, and 7B and its impact on recombination. Theor Appl Genet 91: 282-288.
9. Diretto G, Tavazza R, Welsch R, Pizzichini D, Mourgues F, Papacchioli V, Beyer P, Giuliano G (2006) Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. BMC Plant Biol 6: 13.
10. Diretto G, Welsch R, Tavazza R, Mourgues F, Pizzichini D, Beyer P, Giuliano G (2007) Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers. BMC Plant Biol 7: 11.
11. Du H, Wang N, Cui F, Li X, Xiao J, Xiong L (2010) Characterization of the β-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Plant Physiol 154: 1304-1318.
12. Dubcovsky J, Dvorak J (2007) Genome plasticity a key factor in the success of polyploid wheat under domestication. Science 316: 1862-1866.
13. Dvorak J, Akhunov E (2005) Tempos of gene locus deletions and duplications and their relationship to recombination rate during piploid and polyploid evolution in the Aegilops-Triticum alliance. Genetics 171: 323-332.
14. Dvorak J, McGuire P, Cassidy B (1988) Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30: 680-689.
15. Edgar R (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl Acids Res 32: 1792-1797.
16. Fiore A, Dall'osto L, Fraser PD, Bassi R, Giuliano G (2006) Elucidation of the β-carotene hydroxylation pathway in Arabidopsis thaliana. FEBS Lett 580: 4718-4722.
17. Fiore A, Dall'osto L, Cazzaniga S, Diretto G, Giuliano G, Bassi R (2012) A quadruple mutant of Arabidopsis reveals a β-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio. BMC Plant Biol 12: 50.
18. Galpaz N, Ronen G, Khalfa Z, Zamir D, Hirschberg J (2006) A chromoplast-specific carotenoid biosynthesis pathway is revealed by cloning of the tomato white-flower locus. Plant Cell 18: 1947-1960.
19. Harjes C, Rocheford T, Bai L, Brutnell T, Kandianis C, Sowinski S, Stapleton A, Vallabhaneni R, Williams M, Wurtzel E, Yan J, Buckler E (2008) Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science 319: 330-333.
20. Hentschel V, Kranl K, Hollmann J, Lindhauer M, Bohm V, Bitsch R (2002) Spectrophotometric determination of yellow pigment content and evaluation of carotenoids by high-performance liquid chromatography in durum wheat grain. J Agric Food Chem 50: 6663-6668.
21. Howitt C, Cavanagh C, Bowerman A, Cazzonelli C, Rampling L, Mimica J, Pogson B (2009) Alternative splicing, activation of cryptic exons and amino acid substitutions in carotenoid biosynthetic genes are associated with lutein accumulation in wheat endosperm. Funct Integr Genomics 9: 363-376.
22. Kim J, Smith J, Tian L, DellaPenna D (2009) The evolution and function of carotenoid hydroxylases in Arabidopsis. Plant Cell Physiol 50: 463-479.
23. Lang M, Orgogozo V (2011) Identification of homologous gene sequences by PCR with degenerate primers. Methods Mol Biol 772: 245-256.
24. Laur L, Tian L (2011) Provitamin A and vitamin C content in selected California-grown cantaloupe and honeydew melons and imported melons. J Food Comp Anal 24: 194-201.
25. Lokstein H, Tian L, Polle JEW, DellaPenna D (2002) Xanthophyll biosynthetic mutants of Arabidopsis thaliana: altered nonphotochemical quenching of chlorophyll fluorescence is due to changes in Photosystem II antenna size and stability. Biochim Biophys Acta 1553: 309-319.
26. Naqvi S, Zhu C, Farre G, Sandmann G, Capell T, Christou P (2011) Synergistic metabolism in hybrid corn indicates bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin. Plant Biotechnol J 9: 384-393.
27. Nomura T, Ishihara A, Yanagita R, Endo T, Iwamura H (2005) Three genomes differentially contribute to the biosynthesis of benzoxazinones in hexaploid wheat. Proc Natl Acad Sci USA 102: 16490-16495.
28. 2: oil and defatted cake characterization. J Am Oil Chem Soc 80: 157-161.
29. Paolacci A, Tanzarella O, Porceddu E, Ciaffi M (2009) Identification and validation of reference genes for quantitative RT-PCR normalization in wheat. BMC Mol Biol 10: 11.
30. Schiedt K, Liaaen-Jensen S (1995) Isolation and analysis In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, Vol. 1A: Isolation and analysis. Birkhauser, Basel, Switzerland pp 81-108.
31. Schwartz S, Qin X, Zeevaart J (2001) Characterization of a novel carotenoid cleavage dioxygenase from plants. J Biol Chem 276: 25208-25211.
32. Sorrells M, La Rota M, Bermudez-Kandianis C, Greene R, Kantety R, Munkvold J, Mahmoud A, Miftahudin XF, Ma X, Gustafson P, Qi L, Echalier B, Gill B, Matthews D, Lazo G, Chao S, Anderson O, Edwards H, Linkiewicz A, Dubcovsky J, Akhunov E, Dvorak J, Zhang D, Nguyen H, Peng J, Lapitan N, Gonzalez-Hernandez J, Anderson J, Hossain K, Kalavacharla V, Kianian S, Choi D, Close T, Dilbirligi M, Gill K, Steber C, Walker-Simmons M, McGuire P, Qualset C (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13: 1818-1827.
33. Sun Z, Gantt E, Cunningham FX Jr (1996) Cloning and functional analysis of the β-carotene hydroxylase of Arabidopsis thaliana. J Biol Chem 271: 24349-24352.
34. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739.
35. Taylor J, Raes J (2004) Duplication and divergence: the evolution of new geens and old ideas. Annu Rev Genet 38: 615-643.
36. Tian L, DellaPenna D (2001) Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationships to the LUT1 locus. Plant Mol Biol 45: 379-388.
37. Tian L, Magallanes-Lundback M, Musetti V, DellaPenna D (2003) Functional analysis of β- and ε-ring carotenoid hydroxylases in Arabidopsis thaliana. Plant Cell 15: 1320-1332.
38. Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D (2004) The Arabidopsis LUT1 locus encodes a member of the cytochrome P450 family that is required for carotenoid ε-ring hydroxylation activity. Proc Natl Acad Sci USA 101: 402-407.
39. Uauy C, Paraiso F, Colasuonno P, Tran R, Tsai H, Berardi S, Comai L, Dubcovsky J (2009) A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol 9: 115.
40. Vallabhaneni R, Gallagher CE, Licciardello N, Cuttriss AJ, Quinlan RF, Wurtzel ET (2009) Metabolite sorting of a germplasm collection reveals the Hydroxylase3 locus as a new target for maize provitamin A biofortification. Plant Physiol 151: 1635-1645.
41. Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84: 61-66.
42. Wan Y, Poole R, Huttly A, Toscano-Underwood C, Feeney K, Welham S, Gooding M, Mills C, Edwards K, Shewry P, Mitchell R (2008) Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics 9: 121.
43. Yan J, Kandianis C, Harjes C, Bai L, Kim E, Yang X, Skinner D, Fu Z, Mitchell S, Li Q, Fernandez M, Zaharieva M, Babu R, Fu Y, Palacios N, Li J, Dellapenna D, Brutnell T, Buckler E, Warburton M, Rocheford T (2010) Rare genetic variation at Zea mayscrtRB1 increases beta-carotene in maize grain. Nat Genet 42: 322-327.