EC50 estimation of antioxidant activity in DPPH* assay using several statistical programs

Food Chemistry

Volumn 138, Issue 1, 2013, Pages 414-420

  Chen, Zheng ^a   Bertin, Riccardo ^a   Froldi, Guglielmina ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

Antioxidant activity; DPPH*assay; EC50 estimation; Statistical programs

Indexed keywords

ACETYL-CYSTEINE; ANTIOXIDANT ACTIVITIES; ANTIOXIDANT CAPACITY; ANTIOXIDANT EFFECT; ASCORBIC ACIDS; BIOLOGICAL SUBSTRATES; CAFFEIC ACIDS; CHLOROGENIC ACIDS; COMPARATIVE APPROACH; RADICAL SCAVENGING ACTIVITY; REGRESSION MODEL; SPECIALIZED COMPUTER PROGRAMS;

ESTIMATION; FATTY ACIDS; FLAVONOIDS; FREE RADICALS; ORGANIC ACIDS; PHENOLS; PRISMS; REGRESSION ANALYSIS;

AGENTS;

1,1 DIPHENYL 2 PICRYLHYDRAZYL; ACETYLCYSTEINE; ASCORBIC ACID; CAFFEIC ACID; CATECHIN; CHLOROGENIC ACID; QUERCETIN; 2,2-DIPHENYL-1-PICRYLHYDRAZYL; ANTIOXIDANT; BIPHENYL DERIVATIVE; FREE RADICAL; PICRIC ACID;

ANTIOXIDANT ACTIVITY; ARTICLE; COMPARATIVE STUDY; COMPUTER PROGRAM; DRUG SCREENING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MEDIAN EFFECTIVE CONCENTRATION; PHARMACOLOGICAL PARAMETERS; CHEMISTRY; REGRESSION ANALYSIS;

ANTIOXIDANTS; BIPHENYL COMPOUNDS; FREE RADICALS; PICRATES; REGRESSION ANALYSIS; SOFTWARE;

EID: 84870167870     PISSN: 03088146     EISSN: 18737072     Source Type: Journal    
DOI: 10.1016/j.foodchem.2012.11.001     Document Type: Article

References (31)

1. B. Alexander, D.J. Browse, S.J. Reading, and I.S. Benjamin A simple and accurate mathematical method for calculation of the EC50 Journal of Pharmacological and Toxicological Methods 41 2-3 1999 55 58
2. R. Apak, K. Güçlü, M. Ozyürek, and S.E. Karademir Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method Journal of Agricultural and Food Chemistry 52 26 2004 7970
3. P. Armitage, G. Berry, and J.N.S. Matthews Statistical methods in medical research 2005 Blackwell Science Oxford
4. I.F. Benzie, and J.J. Strain The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay Analytical Biochemistry 239 1 1996 70 76
5. J. Berkson Application to the logistic function to bio-assay Journal of the American Statistical Association 39 227 1944 357 365
6. C.I. Bliss The calculation of the dosage-mortality curve Annals of Applied Biology 22 1 1935 134 167
7. M.S. Blois Antioxidant determinations by the use of a stable free radical Nature 181 4617 1958 1199 1200
8. O. Blokhina, E. Virolainen, and K.V. Fagerstedt Antioxidants, oxidative damage and oxygen deprivation stress: A review Annals of Botany 91 2 2003 179 194
9. V. Bondet, W. Brand-Williams, and C. Berset Kinetics and mechanisms of antioxidant activity using the DPPH* free radical method LWT-Food Science and Technology 30 6 1997 609 615
10. W. Brand-Williams, M.E. Cuvelier, and C. Berset Use of a free radical method to evaluate antioxidant activity LWT-Food Science and Technology 28 1995 25 30
11. G. Cao, H.M. Alessio, and Cutler Oxygen-radical absorbance capacity assay for antioxidants Free Radical Biology & Medicine 14 3 1993 303 311
12. S. Chevion, M.A. Roberts, and M. Chevion The use of cyclic voltammetry for the evaluation of antioxidant capacity Free Radical Biology & Medicine 28 6 2000 860 870
13. A. Christopoulos Assessing the distribution of parameters in models of ligand-receptor interaction: To log or not to log Trends in Pharmacological Sciences 19 9 1998 351 357
14. P.C. Eklund, O.K. Långvik, J.P. Wärnå, T.O. Salmi, S.M. Willför, and R.E. Sjöholm Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans Organic & Biomolecular Chemistry 3 18 2005 3336 3347
15. M.I. Gil, F.A. Tomás-Barber, B. Hess-Pierce, D.M. Holcroft, and A.A. Kader Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing Journal of Agricultural and Food Chemistry 48 10 2000 4581 4589
16. A.N. Glazer Phycoerythrin fluorescence-based assay for reactive oxygen species Methods in Enzymology 186 1990 161 168
17. L.F. Knudsen, and J.M. Curtis The use of the angular transformation in biological assays Journal of the American Statistical Association 42 238 1947 282 296
18. D. Krishnaiah, R. Sarbatly, and R. Nithyanandam A review of the antioxidant potential of medicinal plant species Food and Bioproducts Processing 89 3 2011 217 233
19. M. Locatelli, R. Gindro, F. Travaglia, J.-D. Coïsson, M. Rinaldi, and M. Arlorio Study of the DPPH center dot-scavenging activity: Development of a free software for the correct interpretation of data Food Chemistry 114 3 2009 889 897
20. N.J. Miller, C. Rice-Evans, M.J. Davies, V. Gopinathan, and A. Milner A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates Clinical Science 84 4 1993 407 412
21. K. Mishra, H. Ojha, and N.K. Chaudhury Estimation of antiradical properties of antioxidants using DPPH* assay: A critical review and results Food Chemistry 130 2012 1036 1043
22. H.J. Motulsky, and L.A. Ransnas Fitting curves to data using nonlinear regression: A practical and nonmathematical review FASEB Journal 1 5 1987 365 374
23. N. Nenadis, O. Lazaridou, and M.Z. Tsimidou Use of reference compounds in antioxidant activity assessment Journal of Agricultural and Food Chemistry 55 14 2007 5452 5460
24. M.J. Norusis SPSS 11.0 guide to data analysis 2002 Prentice Hall Upper Saddle River, NJ
25. R.L. Prior, X. Wu, and K. Schaich Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements Journal of Agricultural and Food Chemistry 53 10 2005 4290 4302
26. A. Scalbert, I.T. Johnson, and M. Saltmarsh Polyphenols: Antioxidants and beyond The American Journal of Clinical Nutrition 81 1 2005 215S 217S
27. O.P. Sharma, and T.K. Bhat DPPH antioxidant assay revisited Food Chemistry 113 4 2009 1202 1205
28. J. Tabart, C. Kevers, J. Pincemail, J.-O. Defraigne, and J. Dommes Comparative antioxidant capacities of phenolic compounds measured by various tests Food Chemistry 113 4 2009 1226 1233
29. M. Valko, D. Leibfritz, J. Moncol, M.T. Cronin, M. Mazur, and J. Telser Free radicals and antioxidants in normal physiological functions and human disease The International Journal of Biochemistry & Cell Biology 39 1 2007 44 84
30. P.H. Van der Graaf, and R.C. Schoemaker Analysis of asymmetry of agonist concentration-effect curves Journal of Pharmacological and Toxicological Methods 41 2-3 1999 107 115
31. D. Villano, M.S. Fernández-Pachon, A.M. Troncoso, and M.C. García-Parrilla Comparison of antioxidant activity of wine phenolic compounds and metabolites in vitro Analytica Chimica Acta 538 1-2 2005 391 398