Assessment of three commercial DNA extraction kits and a laboratory-developed method for detecting Cryptosporidium and Cyclospora in raspberry wash, basil wash and pesto

Journal of Microbiological Methods

Volumn 92, Issue 1, 2013, Pages 51-58

  Shields, Joan M ^a   Joo, Jane ^b   Kim, Richard ^c   Murphy, Helen R ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b Joint Institute for Food Safety and Applied Nutrition   (United States)

^c Kyungpook National University   (South Korea)

Author keywords

Cryptosporidium parvum; Cyclospora cayetanensis; DNA extraction; Foods; PCR

Indexed keywords

HEAT SHOCK PROTEIN 70; RNA 18S;

ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; BASIL; CONTROLLED STUDY; CRYPTOSPORIDIUM; CYCLOSPORA; DNA DETERMINATION; DNA EXTRACTION; DNA EXTRACTION KIT; FOOD ANALYSIS; FOOD CONTAMINATION; FOOD WASHING; GENE; HSP70 GENE; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MICROORGANISM DETECTION; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITATIVE RESEARCH; QUANTITATIVE STUDY; RASPBERRY; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE;

CRYPTOSPORIDIUM PARVUM; CYCLOSPORA; DNA, PROTOZOAN; FOOD MICROBIOLOGY; HUMANS; PARASITOLOGY; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SPECIMEN HANDLING; TIME FACTORS;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; CYCLOSPORA; CYCLOSPORA CAYETANENSIS; OCIMUM; PROTOZOA; RUBUS GLAUCUS;

EID: 84870165277     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.11.001     Document Type: Article

References (29)

1. Ariefdjohan M.W., Savaiano D.A., Nakatsu C.H. Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens. Nutr. J. 2010, 22:23.
2. Arrowood M.J. In vitro cultivation of Cryptosporidium Species. Clin. Microbiol. Rev. 2002, 15:390-400.
3. Arrowood M.J., Donaldson K. Improved purification methods for calf-derived Cryptosporidium parvum oocysts using discontinuous sucrose and cesium chloride gradients. J. Eukaryot. Microbiol. 1996, 43:89.
4. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M.W., Shipley G.L., Vandesompele J., Wittwer C.T. The MIQE Guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55:611-622.
5. CDC Outbreak of cyclosporiasis - Northern Virginia-Washington, D.C.-Baltimore, Maryland, Metropolitan Area, 1997. Morb. Mortal. Wkly. Rep. 1997, 46:689-691.
6. Chen X.-M., Keithly J.S., Paya C.V., LaRusso N.F. Cryptosporidiosis. N. Engl. J. Med. 2002, 346:1723-1731.
7. Connor B.A., Reidy J., Soave R. Cyclosporiasis: clinical and histopathologic correlates. Clin. Infect. Dis. 1999, 28:1216-1222.
8. Current W.L., Garcia L.S. Cryptosporidiosis. Clin. Microbiol. Rev. 1991, 4:325-358.
9. FDA BAM Bacteriological Analytical Manual 2009, FDA, Washington, DC.
10. Grant M.A. Evaluation of methods to improve detection of Escherichia coli O157:H7 in fresh produce by multiplex polymerase chain reaction. J. Food Prot. 2003, 66:18-24.
11. Herwaldt B.L. Cyclospora cayetanensis: a review, focusing on the outbreaks of cyclosporiasis in the 1990s. Emerg. Infect. Dis. 2000, 31:1040-1057.
12. Hill V.R., Jothikumar N., Vinjé J., Cromeans T.L. Sample Preparation Methods for Molecular Techniques for Drinking Water 2010, Water Research Foundation.
13. Ho A.Y., Lopez A.S., Eberhart M.G., Levenson R., Finkel B.S., daSilva A.J., Roberts J.M., Orlandi P.A., Johnson C.C., Herwaldt B.L. Outbreak of cyclosporiasis associated with imported raspberries, Philadelphia, Pennsylvania, 2000. Emerg. Infect. Dis. 2002, 8:783-788.
14. Hoang L., Fyee M., Ong C., Harb J., Champagne S., Dixon B., Isaac-Renton J. Outbreak of cyclosporiasis in British Columbia associated with imported Thai basil. Epidemiol. Infect. 2005, 131:23-27.
15. Insulander M., Svenungsson B., Lebbad M., Karlsson L., de Jong B. A foodborne outbreak of Cyclospora infection in Stockholm, Sweden. Foodborne Pathog. Dis. 2010, 7:1585-1587.
16. Jiang J., Alderisio K.A., Singh A., Xiao L. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl. Environ. Microbiol. 2005, 71:1135-1141.
17. Khramtsov N.V., Tilley M., Blunt D.S., Montelone B.A., Upton S.J. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 1995, 42:416-422.
18. Lalonde L.F., Gajadhar A.A. Detection and differentiation of coccidian oocysts by real-time PCR and melting curve analysis. J. Parasitol. 2011, 97:725-730.
19. Lopez A.S., Dodson D.R., Arrowood M.J., Orlandi P.A., da Silva A.J., Bier J.W., Hanauer S.D., Kuster R.L., Oltman S., Baldwin M.S., Won K.Y., Nace E.M., Eberhard M.L., Herwaldt B.L. Outbreak of cyclosporiasis associated with basil in Missouri in 1999. Clin. Infect. Dis. 2001, 32:1010-1017.
20. Moss T., Stefanovsky V.Y. At the center of eukaryotic life. Cell 2002, 109:545-548.
21. Ortega Y.R., Sterling C.R., Gilman R.H., Cama V.A., Diaz F. Cyclospora species - a new protozoan pathogen of humans. N. Engl. J. Med. 1993, 328:1308-1312.
22. Pape J.W., Verdier R.-I., Boncy M., Boncy J., Johnson W.D. Cyclospora infection in adults infected with HIV. Clinical manifestations, treatment, and prophylaxis. Ann. Intern. Med. 1994, 121:654-657.
23. Rossmanith P., Wagner M. A novel Poisson distribution-based approach for testing boundaries of real-time PCR assays for food pathogen quantification. J. Food Prot. 2011, 74:1404-1412.
24. Shields J.M., Olson B.H. PCR-restriction fragment length polymorphism method for detection of Cyclospora cayetanensis in environmental waters without microscopic confirmation. Appl. Environ. Microbiol. 2003, 69:4662-4669.
25. Shields J.M., Lee M.M., Murphy H.R. Use of a common laboratory glassware detergent improves recovery of Cryptosporidium parvum and Cyclospora cayetanensis from lettuce, herbs and raspberries. Int. J. Food Microbiol. 2012, 153:123-128.
26. Smith H.V., Nichols R.A.B. Cryptosporidium: detection in water and food. Exp. Parasitol. 2010, 124:19.
27. Steele M., Unger S., Odumeru J. Sensitivity of PCR detection of Cyclospora cayetanensis in raspberries, basil, and mesclun lettuce. J. Microbiol. Methods 2003, 54:277-280.
28. Sunnotel O., Lowery C.J., Moore J.E., Dooley J.S.G., Xiao L., Millar B.C.R., Rooney P.J., Snelling W.J. Cryptosporidium and public health. Lett. Appl. Microbiol. 2006, 43:10.
29. Weiss L.M., Ma Y.F., Takvorian P.M., Tanowitz H.B., Murray W. Bradyzoite development in Toxoplasma gondii and the hsp70 stress response. Infect. Immun. 1998, 66:3295-3302.