Observing and modeling BMCC degradation by commercial cellulase cocktails with fluorescently labeled Trichoderma reseii Cel7A through confocal microscopy

Biotechnology and Bioengineering

Volumn 110, Issue 1, 2013, Pages 108-117

  Luterbacher, Jeremy S ^a   Walker, Larry P ^b   Moran Mirabal, Jose M ^c  

^a Cornell University   (United States)

^b Department of Biological and Environmental Engineering   (United States)

^c MCMASTER UNIVERSITY   (Canada)

Author keywords

Biofuels; Cellulase; Cellulose; Confocal microscopy; Enzymatic hydrolysis; Fluorescence microscopy; Kinetic modeling

Indexed keywords

BIOFUELS; CELLULOSE; CONFOCAL MICROSCOPY; CRYSTALLINE MATERIALS; ENZYMATIC HYDROLYSIS; ENZYME ACTIVITY; EXPERIMENTS; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GLUCOSE; KINETIC THEORY; KINETICS; OPTIMIZATION; REACTION KINETICS;

BACTERIAL MICROCRYSTALLINE CELLULOSE; BINDING MODELS; CELLULASE; CELLULOSIC SUBSTRATES; CRITICAL STEPS; CRYSTALLINE CELLULOSE; ENZYME MIXTURES; FLUORESCENCE INTENSITIES; FLUORESCENT DYES; GLASS SURFACES; GLUCOSIDASE; HYDROLYTIC ACTIVITIES; KINETIC MODELING; KINETIC MODELS; MICRO-SCALE IMAGING; MORPHOLOGICAL CHANGES; OPTIMAL TEMPERATURE; PHYSICAL STRUCTURES; RATE-LIMITING STEPS; REVERSIBLE BINDING; SOLUBLE SUGARS; SPEZYME CP; TRICHODERMA;

SUBSTRATES;

BACTERIAL MICROCRYSTALLINE CELLULOSE; BETA GLUCOSIDASE; CELLULASE; CELLULOSE; FLUORESCENT DYE; GLASS; MONOSACCHARIDE; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DEGRADATION; DEPOLYMERIZATION; ENZYME BINDING; FLUORESCENCE; FRACTIONATION; HYDROLYSIS; IMAGE PROCESSING; KINETICS; MORPHOLOGY; NONHUMAN; TEMPERATURE; TRICHODERMA; TRICHODERMA RESEII;

BACTERIA (MICROORGANISMS); TRICHODERMA;

EID: 84869890173     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24597     Document Type: Article

References (31)

1. Banerjee G, Car S, Scott-Craig JS, Borrusch MS, Aslam N, Walton JD. 2010a. Synthetic enzyme mixtures for biomass deconstruction: Production and optimization of a core set. Biotechnol Bioeng 106: 707-720.
2. Banerjee G, Car S, Scott-Craig JS, Borrusch MS, Walton JD. 2010b. Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations. Biotechnol Biofuels 3: 1-15.
3. Bansal P, Hall M, Realff MJ, Lee JH, Bommarius AS. 2009. Modeling cellulase kinetics on lignocellulosic substrates. Biotechnol Adv 27: 833-848.
4. Bothwell MK, Daughhetee SD, Chaua GY, Wilson DB, Walker LP. 1997. Binding capacities for thermomonospora fusca e3, e4 and e5, the e3 binding domain, and trichoderma reseii cbhi on avicel and bacterial microcrystalline cellulose. Bioresour Technol 60: 169-178.
5. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
6. Fox JM, Levine SE, Clark DS, Blanch HW. 2011. Initial- and processive-cut products reveal cellobiohydrolase rate limitations and the role of companion enzymes. Biochemistry 51: 442-452.
7. Grethlein HE. 1985. The effect of pore-size distribution on the rate of enzymatic-hydrolysis of cellulosic substrates. Nat Biotech 3: 155-160.
8. Helbert W, Chanzy H, Husum TL, Schülein M, Ernst S. 2003. Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity. Biomacromolecules 4: 481-487.
9. Houghton J. 2005. Breaking the biological barriers to cellulosic ethanol: A joint research agenda. Rockville, MD: US Department of Energy.
10. Husson E, Buchoux S, Avondo C, Cailleu D, Djellab K, Gosselin I, Wattraint O, Sarazin C. 2011. Enzymatic hydrolysis of ionic liquid-pretreated celluloses: Contribution of cp-mas 13c nmr and sem. Bioresour Technol 102: 7335-7342.
11. Igarashi K, Koivula A, Wada M, Kimura S, Penttilä M, Samejima M. 2009. High speed atomic force microscopy visualizes processive movement of trichoderma reesei cellobiohydrolase i on crystalline cellulose. J Biol Chem 284: 36186.
12. Igarashi K, Uchihashi T, Koivula A, Wada M, Kimura S, Okamoto T, Penttilä M, Ando T, Samejima M. 2011. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science 333: 1279-11282.
13. Irwin DC, Spezio M, Walker LP, Wilson DB. 1993. Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects. Biotechnol Bioeng 42: 1002-1013.
14. Jeoh T, Ishizawa CI, Davis MF, Himmel ME, Adney WS, Johnson DK. 2007. Cellulase digestibility of pretreated biomass is limited by cellulose accessibility. Biotechnol Bioeng 98: 112-122.
15. Levine SE, Fox JM, Blanch HW, Clark DS. 2010. A mechanistic model of the enzymatic hydrolysis of cellulose. Biotechnol Bioeng 107: 37-51.
16. Levine SE, Fox JM, Clark DS, Blanch HW. 2011. A mechanistic model for rational design of optimal cellulase mixtures. Biotechnol Bioeng 108: 2561-2570.
17. 2O pretreatment of lignocellulosic biomass. Biotechnol Bioeng 107: 451-460.
18. Luterbacher JS, Chew Q, Li Y, Tester JW, Walker LP. 2012a. Producing concentrated solutions of monosaccharides using biphasic CO2-H2O mixtures. Energy Environ 5: 6990-7000.
19. Luterbacher JS, Tester JW, Walker LP. 2012b. Two-temperature stage biphasic CO2-H2O pretreatment of lignocellulosic biomass at high solid loadings. Biotechnol Bioeng 109: 1499-1507.
20. Lynd LR, Laser MS, Brandsby D, Dale BE, Davison B, Hamilton R, Himmel M, Keller M, McMillan JD, Sheehan J, Wyman CE. 2008. How biotech can transform biofuels. Nat Biotech 26: 169-172.
21. Moran-Mirabal JM, Santhanam N, Corgie SC, Craighead HG, Walker LP. 2008. Immobilization of cellulose fibrils on solid substrates for cellulase-binding studies through quantitative fluorescence microscopy. Biotechnol Bioeng 101: 1129-1141.
22. Moran-Mirabal JM, Corgie SC, Bolewski JC, Smith HM, Cipriany BR, Craighead HG, Walker LP. 2009. Labeling and purification of cellulose-binding proteins for high resolution fluorescence applications. Anal Chem 81: 7981-7987.
23. Moran-Mirabal JM, Bolewski JC, Walker LP. 2011. Reversibility and binding kinetics of thermobifida fusca cellulases studied through fluorescence recovery after photobleaching microscopy. Biophys Chem 155: 20-28.
24. Pinto R, Amaral AL, Carvalho J, Ferreira EC, Mota M, Gama M. 2007. Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers. Biotechnol Prog 23: 1492-1497.
25. Selig MJ, Viamajala S, Decker SR, Tucker MP, Himmel ME, Vinzant TB. 2007. Deposition of lignin droplets produced during dilute acid pretreatment of maize stems retards enzymatic hydrolysis of cellulose. Biotechnol Prog 23: 1333-1339.
26. Selig MJ, Weiss N, Ji Y. 2008. Enzymatic saccharification of lignocellulosic biomass. Golden, CO: National Renewable Energy Laboratory, TP-510-42629. NREL Analytical Procedure.
27. Singh S, Simmons BA, Vogel KP. 2009. Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass. Biotechnol Bioeng 104: 68-75.
28. Thevenaz P, Ruttimann UE, Unser M. 1998. A pyramid approach to subpixel registration based on intensity. IEEE Trans 7: 27-41.
29. Wyman CE, Dale BE, Elander RT, Holtzapple M, Ladisch MR, Lee YY. 2005a. Coordinated development of leading biomass pretreatment technologies. Bioresour Technol 96: 1959-1966.
30. Wyman CE, Dale BE, Elander RT, Holtzapple M, Ladisch MR, Lee YY. 2005b. Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover. Bioresour Technol 96: 2026-2032.
31. Zhu P, Moran-Mirabal JM, Luterbacher JS, Walker LP, Craighead HG. 2011. Observing thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy. Cellulose 18: 749-758.