Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.

Molecular and Cellular Probes

Volumn 27, Issue 1, 2013, Pages 68-70

  Vitullo, Monia ^a,b   Grant, Kathie Ann ^b   Sammarco, Michela Lucia ^a   Tamburro, Manuela ^a   Ripabelli, Giancarlo ^a   Amar, Corinne Francoise Laurence ^b  

^a UNIVERSITY OF MOLISE   (Italy)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Listeria monocytogenes; Listeria spp.; Molecular epidemiology; Real time PCR; Serogrouping

Indexed keywords

EXONUCLEASE;

ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BLOOD GROUP TYPING; CONTROLLED STUDY; LISTERIA; LISTERIA GRAYI; LISTERIA INNOCUA; LISTERIA IVANOVII; LISTERIA MONOCYTOGENES; LISTERIA SEELIGERI; LISTERIA WELSHIMERI; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DNA, BACTERIAL; FOOD MICROBIOLOGY; LISTERIA MONOCYTOGENES; REAL-TIME POLYMERASE CHAIN REACTION; SEROTYPING; SPECIES SPECIFICITY;

LISTERIA; LISTERIA MONOCYTOGENES;

EID: 84869868071     PISSN: 08908508     EISSN: 10961194     Source Type: Journal    
DOI: 10.1016/j.mcp.2012.10.001     Document Type: Article

References (11)

1. Seeliger H.P., Höhne K. Serotyping of Listeria monocytogenes and related species. Methods Microbiol 1979, 13:31-49.
2. Leclercq A., Chenal-Francisque V., Dieye H., Cantinelli T., Drali R., Brisse S., et al. Characterization of the novel Listeria monocytogenes PCR serogrouping profile IVb-v1. Int J Food Microbiol 2011, 147:74-77.
3. Tamburro M., Ripabelli G., Fanelli I., Grasso G.M., Sammarco M.L. Typing of Listeria monocytogenes strains isolated in Italy by inlA gene characterization and evaluation of a new cost-effective approach to antisera selection for serotyping. J Appl Microbiol 2010, 108:1602-1611.
4. Doumith M., Buchrieser C., Glaser P., Jacquet C., Martin P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004, 42:3819-3822.
5. Huang B., Fang N., Dimovski K., Wang X., Hogg G., Bates J. Observation of a new pattern in serogroup-related PCR typing of Listeria monocytogenes 4b isolates. J Clin Microbiol 2011, 49:426-429.
6. den Bakker H.C., Cummings C.A., Ferreira V., Vatta P., Orsi R.H., Degoricija L., et al. Comparative genomics of the bacterial genus Listeria: genome evolution is characterized by limited gene acquisition and limited gene loss. BMC Genomics 2010, 11:688.
7. Graves L.M., Helsel L.O., Steigerwalt A.G., Morey R.E., Daneshvar M.I., Roof S.E., et al. Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest. Int J Syst Evol Microbiol 2012, 60:1280-1288.
8. Leclercq A., Clermont D., Bizet C., Grimont P.A., Le Flèche-Matéos A., Roche S.M., et al. Listeria rocourtiae sp. nov. Int J Syst Evol Microbiol 2010, 60:2210-2214.
9. Bertsch D., Rau J., Eugster M.R., Haug M.C., Lawson P.A., Lacroix C., et al. Listeria fleischmannii sp. nov., isolated from cheese. Int J Syst Evol Microbiol 2012, [Epub ahead of print]. 10.1099/ijs.0.036947-0.
10. Kérouanton A., Marault M., Petit L., Grout J., Dao T.T., Brisabois A. Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping. J Microbiol Methods 2010, 80:134-137.
11. Amar C. Molecular diagnosis of gastrointestinal pathogens. Molecular diagnostics of infectious diseases 2010, 135-154. de Gruyter GmbH & Co, Berlin, Germany. H.H. Kessler (Ed.).