One-day workflow scheme for bacterial pathogen detection and antimicrobial resistance testing from blood cultures

Journal of Visualized Experiments

Volumn , Issue 65, 2012, Pages

  Hansen, Wendy L J ^a   Beuving, Judith ^a   Verbon, Annelies ^b   Wolffs, Petra F G ^a  

^a MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

^b ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

16S rDNA gene; Antibiotic resistance; Antibiotic susceptibility testing; Bacteria; Blood culture; Immunology; Infection; Issue 65; Medicine; Microbiology; Pathogen detection; Probes; Real time PCR

Indexed keywords

EID: 84869798078     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3254     Document Type: Article

References (11)

1. Wallet, F., et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin. Microbiol. Infect. 16 (6), 774 (2010).
2. Beekmann, S.E., Diekema, D.J., Chapin, K.C., & Doern, G.V. Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J. Clin. Microbiol. 41 (7), 3119 (2003).
3. Raymaekers, M., Bakkus, M., Boone, E., de Rijke, B., El Housni, H., Descheemaeker, P., De Schouwer, P., Franke, S., Hillen, F., Nollet, F., Soetens, O., Vankeerberghen, A. Molecular Diagnostics working group. Reflections and proposals to assure quality in molecular diagnostics. Acta. Clin. Belg. 66 (1), 33 (2011).
4. Peters, R.P., et al. New developments in the diagnosis of bloodstream infections. Lancet Infect. Dis. 4 (12), 751 (2004).
5. Hansen, W.L., Beuving, J., Bruggeman, C.A., & Wolffs, P.F. Molecular probes for the diagnosis of clinically relevant bacterial infections in blood cultures. J. Clin. Microbiol. 48 (12), 4432 (2010).
6. Beuving, J., et al. Antibiotic susceptibility testing of grown blood cultures by combining culture and real-time polymerase chain reaction is rapid and effective. PLoS ONE. 6 (12), (2011).
7. Rolain, J.M., Mallet, M.N., Fournier, P.E., & Raoult, D. Real-time PCR for universal antibiotic susceptibility testing. J. Antimicrob. Chemother. 54 (2), 538 (2004).
8. Nadkarni, M.A., Martin, F.E., Jacques, N.A., & Hunter, N. Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiol. 148 (Pt 1), 257 (2002).
9. Waites, K.B., Brookings, E.S., Moser, S.A., & Zimmer, B.L. Direct susceptibility testing with positive BacT/Alert blood cultures by using MicroScan overnight and rapid panels. J. Clin. Microbiol. 36 (7), 2052 (1998).
10. Vliegen, I., et al. Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene. J. Microbiol. Meth. 66 (1), 156 (2006).
11. Hall, L., Doerr, K.A., Wohlfiel, S.L., & Roberts, G.D. Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory. J. Clin. Microbiol. 41 (4), 1447 (2003).