Using electrophoretic mobility shift assays to measure equilibrium dissociation constants: GAL4-p53 binding DNA as a model system

Biochemistry and Molecular Biology Education

Volumn 40, Issue 6, 2012, Pages 383-387

  Heffler, Michael A ^a   Walters, Ryan D ^a   Kugel, Jennifer F ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Biochemistry; EMSA; Equilibrium dissociation constant; Protein DNA interaction

Indexed keywords

DNA; DNA BINDING PROTEIN; GAL4 PROTEIN, S CEREVISIAE; HYBRID PROTEIN; PROTEIN P53; SACCHAROMYCES CEREVISIAE PROTEIN; TP53 PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

ANIMAL; ARTICLE; BIOCHEMISTRY; CHEMISTRY; EDUCATION; GEL MOBILITY SHIFT ASSAY; HUMAN; METABOLISM; METHODOLOGY;

ANIMALS; BIOCHEMISTRY; DNA; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HUMANS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84869787724     PISSN: 14708175     EISSN: 15393429     Source Type: Journal    
DOI: 10.1002/bmb.20649     Document Type: Article

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