An in vitro model using the IPEC-J2 cell line for efficacy and drug interaction testing of mycotoxin detoxifying agents

Toxicology in Vitro

Volumn 27, Issue 1, 2013, Pages 157-163

  Devreese, Mathias ^a   Pasmans, Frank ^a   De Backer, Patrick ^a   Croubels, Siska ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Deoxynivalenol; In vitro model; IPEC J2; Mycotoxin binders; Tylosin

Indexed keywords

ACTIVATED CARBON; BENTONITE; FOOD ADDITIVE; MYCOTOXIN; TYLOSIN; VOMITOXIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BINDING AFFINITY; BIOLOGICAL MODEL; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTRIC RESISTANCE; IN VITRO STUDY; MOLECULAR INTERACTION; NONHUMAN; SCREENING TEST;

ANIMALS; BENTONITE; CARBON; CELL LINE; CELL SURVIVAL; DRUG INTERACTIONS; MANNANS; MYCOTOXINS; SWINE; TRICHOTHECENES; TYLOSIN;

SUS;

EID: 84869394325     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2012.09.020     Document Type: Article

References (27)

1. Avantaggiato G., Havenaar R., Visconti A. Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials. Food and Chemical Toxicology 2004, 42:817-824.
2. Awad W.A., Aschenbach J.R., Setyabudi F.M., Razzazi-Fazeli E., Bohm J., Zentek J. In vitro effects of deoxynivalenol on small intestinal d-glucose uptake and absorption of deoxynivalenol across the isolated jejunal epithelium of laying hens. Poultry Science 2007, 86:15-20.
3. Cavret S., Laurent N., Videmann B., Mazallon M., Lecoeur S. Assessment of deoxynivalenol (DON) adsorbents and characterisation of their efficacy using complementary in vitro tests. Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment 2010, 27:43-53.
4. Suspected drug adverse reactions reported to The Bureau of Veterinary Drugs. Canadian Veterinary Journal 1992, 33:237-244. Canadian Bureau of Veterinary Drugs.
5. Dänicke S., Hegewald A.K., Kahlert S., Kluess J., Rothkotter H.J., Breves G., Doll S. Studies on the toxicity of deoxynivalenol (DON), sodium metabisulfite, DON-sulfonate (DONS) and de-epoxy-DON for porcine peripheral blood mononuclear cells and the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2, and on effects of DON and DONS on piglets. Food and Chemical Toxicology 2010, 48:2154-2162.
6. Devreese M., Osselaere A., Goossens J., Vandenbroucke V., De Baere S., Eeckhout M., De Backer P., Croubels S. New bolus models for in vivo efficacy testing of mycotoxin-detoxifying agents in relation to EFSA guidelines, assessed using deoxynivalenol in broiler chickens. Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment 2012, 29:1101-1107.
7. Diesing A.K., Nossol C., Danicke S., Walk N., Post A., Kahlert S., Rothkotter H.J., Kluess J. Vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application. Plos One 2011, 6.
8. Diesing A.K., Nossol C., Panther P., Walk N., Post A., Kluess J., Kreutzmann P., Danicke S., Rothkotter H.J., Kahlert S. Mycotoxin deoxynivalenol (DON) mediates biphasic cellular response in intestinal porcine epithelial cell lines IPEC-1 and IPEC-J2. Toxicology Letters 2011, 200:8-18.
9. EFSA, 2009. Review of mycotoxin-detoxifying agents used as feed additives: mode of action, efficacy and feed/food safety. Available from: .
10. Statement on the establishment of guidelines for the assessment of additives from the functional group 'substances for reduction of the contamination of feed by mycotoxins'. EFSA Journal 2010, 8:1693. EFSA.
11. Scientific opinion on the safety and efficacy of bentonite (dioctahedral montmorillonite) as feed additive for all species. EFSA Journal 2011, 9:2007. EFSA.
12. Eriksen G.S., Pettersson H., Lindberg J.E. Absorption, metabolism and excretion of 3-acetyl DON in pigs. Archives of Animal Nutrition-Archiv Fur Tierernahrung 2003, 57:335-345.
13. Commission Decision 2002/675/EC implementing Council Directive 96/23/EC concerning the performances of analytical methods and the interpretation of results. Official Journal of the European Commission 2002, L221. European Commission.
14. Commision Regulation 386/2009/EC of 12 May 2009 amending Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the establishment of a new functional group of feed additives. Official Journal of the European Union 2009, L118:66. European Commission.
15. Geens M.M., Niewold T.A. Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization. Cytotechnology 2011, 63:415-423.
16. Heitzman, R.J., 1994. Veterinary Drug Residues, Report Eur. 14126-EN, Commission of the EC, Brussels-Luxembourg, 1994.
17. Kolosova A., Stroka J. Substances for reduction of the contamination of feed by mycotoxins: a review. World Mycotoxin Journal 2011, 4:225-256.
18. Matuszewski B.K., Constanzer M.L., Chavez-Eng C.M. Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Analytical Chemistry 2003, 75:3019-3030.
19. Minekus M., Marteau P., Havenaar R., Huis in 't Veld J.H.J. A multicompartmental dynamic computer-controlled model simulating the stomach and small intestine. Alternatives To Laboratory Animals 1995, 23:197-209.
20. Monbaliu S., Van Poucke C., Detavernier C., Dumoulin F., Van De Velde M., Schoeters E., Van Dyck S., Averkieva O., Van Peteghem C., De Saeger S. Occurrence of mycotoxins in feed as analyzed by a multi-mycotoxin LC-MS/MS method. Journal of Agricultural and Food Chemistry 2010, 58:66-71.
21. Pinton P., Braicu C., Nougayrede J.P., Laffitte J., Taranu I., Oswald I.P. Deoxynivalenol impairs porcine intestinal barrier function and decreases the protein expression of claudin-4 through a mitogen-activated protein kinase-dependent mechanism. Journal of Nutrition 2010, 140:1956-1962.
22. Ramos A.J., FinkGremmels J., Hernandez E. Prevention of toxic effects of mycotoxins by means of nonnutritive adsorbent compounds. Journal of Food Protection 1996, 59:631-641.
23. Sergent T., Parys M., Garsou S., Pussemier L., Schneider Y.J., Larondelle Y. Deoxynivalenol transport across human intestinal Caco-2 cells and its effects on cellular metabolism at realistic intestinal concentrations. Toxicology Letters 2006, 164:167-176.
24. Shryock T.R., Klink P.R., Readnour R.S., Tonkinson L.V. Effect of bentonite incorporated in a feed ration with tilmicosin in the prevention of induced Mycoplasma gallisepticum airsacculitis in broiler-chickens. Avian Diseases 1994, 38:501-505.
25. VICH GL 49 (MRK), 2009. Studies to evaluate the metabolism and residue kinetics of veterinary drugs in food producing animals: validation of analytical methods used in residue depletion studies. November 2009, Brussels, Belgium.
26. Wu F. Measuring the economic impacts of Fusarium toxins in animal feeds. Animal Feed Science and Technology 2007, 137:363-374.
27. Yiannikouris A., Andre G., Poughon L., Francois J., Dussap C.G., Jeminet G., Bertin G., Jouany J.P. Chemical and conformational study of the interactions involved in mycotoxin complexation with beta-d-glucans. Biomacromolecules 2006, 7:1147-1155.