Identifi cation of gntK, a gene required for the methylation of purpurosamine C-6′ in gentamicin biosynthesis

Journal of General and Applied Microbiology

Volumn 58, Issue 5, 2012, Pages 349-356

  Hong, Wenrong ^a   Yan, Lingbin ^a  

^a FUZHOU UNIVERSITY   (China)

Author keywords

Gene inactivation; Gentamicin biosynthesis; GntK; Methylase gene; Micromonospora purpurea

Indexed keywords

BACTERIAL ENZYME; GENTAMICIN C; PURPUROSAMINE C 6'; SISOMICIN; UNCLASSIFIED DRUG; GENTAMICIN; HEPTOSE;

AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; CYTOKINE PRODUCTION; GENE AMPLIFICATION; GENE DISRUPTION; GENE IDENTIFICATION; GENE INACTIVATION; GNTK GENE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOMOLOGOUS RECOMBINATION; MASS SPECTROMETRY; METHYLATION; MICROMONOSPORA; MICROMONOSPORA PURPUREA; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; RESTRICTION MAPPING; RESTRICTION SITE; SEQUENCE HOMOLOGY; THIN LAYER CHROMATOGRAPHY; BACTERIUM CONJUGATION; ENZYME ACTIVE SITE; ESCHERICHIA COLI; FERMENTATION; GENETICS; METABOLISM; MOLECULAR WEIGHT; TRANSCRIPTION INITIATION;

MICROMONOSPORA ECHINOSPORA;

CATALYTIC DOMAIN; CONJUGATION, GENETIC; ESCHERICHIA COLI; FERMENTATION; GENES, BACTERIAL; GENTAMICINS; HEPTOSES; METHYLATION; MICROMONOSPORA; MOLECULAR WEIGHT; PLASMIDS; TRANSCRIPTIONAL ACTIVATION;

EID: 84869236077     PISSN: 00221260     EISSN: 13498037     Source Type: Journal    
DOI: 10.2323/jgam.58.349     Document Type: Article

References (20)

1. Aboshanab, K. M. A. (2005) Genetic studies on the biosynthesis of the major aminoglycoside antibiotics. Dissertation, Bergische Wuppertal University.
2. Bierman, M., Logan, R., O'Brien, K., Seno, E. T., Rao, R. N., and Schoner, B. E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene, 116, 43-49.
3. China National Pharmacopoeia Committee (2010) Pharmacopoeia of the People's Republic of China, Part 2. China Medical Science Press, Beijing.
4. Eubank, T. D., Biswas, R., Jovanovic, M., Litovchick, A., Lapidot, A., and Gopalan, V. (2002) Inhibition of bacterial RNase P by aminoglycoside-arginine conjugates. FEBS Lett., 511, 107-112.
5. Hong, W. R. (1994) The optimization of gentamicin fermentation process. Chin. J. Pharmaceut., 25, 1-3.
6. Hong, W. R. and Yan, S. D. (2012) Engineering Streptomyces tenebrarius to synthesize single component of carbamoyl tobramycin. Lett. Appl. Microbiol., 55, 33-39.
7. Hong, W. R., Ge, M., Zeng, Z. H., Zhu, L., Luo, M. Y., Shao, L., and Chen, D. J. (2009) Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis. Biotechnol. Lett., 31, 449-455.
8. Hou, Y. H., Li, F. C., Wang, S. J., Qin, S., and Wang, Q. F. (2008) Intergeneric conjugation in holomycin-producing marine Streptomyces sp. strain M095. Microbiol. Res., 163, 96- 104.
9. Jin, Y. K., Suh, J. W., Kang, S. H., Phan, T. H., Park, S. H., and Kwon, H. J. (2008) Gene inactivation study of gntE reveals its role in the fi rst step of pseudotrisaccharide modifi cations in gentamicin biosynthesis. Biochem. Biophys. Res. Commun., 372, 730-734.
10. Kieser, T. and Foundation, J. I. (2000) Practical Streptomyces Genetics. The John Innes Foundation, Norwich.
11. Litovchick, A., Evdokimov, A. G., and Lapidot, A. (1999) Arginineaminoglycoside conjugates that bind to HIV transactivation responsive element RNA in vitro. FEBS Lett., 445, 73-79.
12. Litovchick, A., Lapidot, A., Eisenstein, M., Kalinkovich, A., and Borkow, G. (2001) Neomycin B-arginine conjugate, a novel HIV-1 Tat antagonist: Synthesis and anti-HIV activities. Biochem. US, 40, 15612-15623.
13. MacNeil, D. J., Occi, J. L., Gewain, K. M., MacNeil, T., Gibbons, P. H., Ruby, C. L., and Danis, S. J. (1992) Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase. Gene, 115, 119- 125.
14. Mazodier, P., Petter, R., and Thompson, C. (1989) Intergeneric conjugation between Escherichia coli and Streptomyces species. J. Bacteriol., 171, 3583-3585.
15. Nepal, K. K., Yoo, J. C., and Sohng, J. K. (2010) Biosynthetic approach for the production of new aminoglycoside derivative. J. Biosci. Bioeng., 110, 109-112.
16. Rogers, J. and Davies, J. (1994) The pseudodisaccharides: A novel class of group I intron splicing inhibitors. Nucleic Acids Res., 22, 4983-4988.
17. Schroeder, R., Waldsich, C., and Wank, H. (2000) Modulation of RNA function by aminoglycoside antibiotics. EMBO J., 19, 1-9.
18. Unwin, J., Standage, S., Alexander, D., Hosted, T. Jr., Horan, A. C., and Wellington, E. M. (2004) Gene cluster in Micromonospora echinospora TCC15835 for the biosynthesis of the gentamicin C complex. J. Antibiot. (Tokyo), 57, 436-445.
19. Wagman, G. H. and Weinstein, M. J. (1980) Antibiotics from Micromonospora. Annu. Rev. Microbiol., 34, 537-557.
20. Weinstein, M. J., Luedemann, G. M., Oden, E. M., Wagman, G. H., Rosselet, J. P., Marquez, J. A., Coniglio, C. T., Charney, W., Herzog, H. L., and Black, J. (1963) Gentamicin, a new antibiotic complex from Micromonospora. J. Med. Chem., 122, 463-464.