A novel method for genotyping the Helicobacter pylori vacA intermediate region directly in gastric biopsy specimens

Journal of Clinical Microbiology

Volumn 50, Issue 12, 2012, Pages 3983-3989

  Ferreira, Rui M ^a,b   Machado, Jose C ^a,b   Letley, Darren ^c   Atherton, John C ^c   Pardo, Maria L ^d   Gonzalez, Carlos A ^e   Carneiro, Fatima ^a,b,f   Figueiredo, Ceu ^a,b  

^a UNIVERSITY OF PORTO   (Portugal)

^b University of Porto   (Portugal)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^d Hospital Virgen del Mirón   (Spain)

^e CATALAN INSTITUTE OF ONCOLOGY   (Spain)

^f Centro Hospitalar Universitário de São João   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; CAGA GENE; CELL VACUOLE; CONTROLLED STUDY; FEMALE; GENE FREQUENCY; GENE SEQUENCE; GENOTYPE; HELICOBACTER PYLORI; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SEX DIFFERENCE; STOMACH BIOPSY; STOMACH CARCINOMA; VACA GENE;

ADULT; AGED; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BIOPSY; DNA, BACTERIAL; FEMALE; GASTRIC MUCOSA; GENOTYPE; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; VIRULENCE FACTORS;

HELICOBACTER PYLORI;

EID: 84869213164     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02087-12     Document Type: Article

References (36)

1. Atherton JC. 2006. The pathogenesis of Helicobacter pylori-induced gastro-duodenal diseases. Annu. Rev. Pathol. 1:63-96.
2. Atherton JC, et al. 1995. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J. Biol. Chem. 270:17771-17777.
3. Backert S, Tegtmeyer N, Selbach M. 2010. The versatility of Helicobacter pylori CagA effector protein functions: The master key hypothesis. Helicobacter 15:163-176.
4. Basso D, et al. 2008. Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms. Gastroenterology 135:91-99. (Pubitemid 351899019)
5. Blaser MJ, Atherton JC. 2004. Helicobacter pylori persistence: biology and disease. J. Clin. Invest. 113:321-333. (Pubitemid 38544173)
6. Boom R, et al. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503. (Pubitemid 20078123)
7. Censini S, et al. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. U. S. A. 93:14648-14653.
8. Chung C, et al. 2010. Diversity of VacA intermediate region among Helicobacter pylori strains from several regions of the world. J. Clin. Microbiol. 48:690-696.
9. Correa P. 1992. Human gastric carcinogenesis: a multistep and multifactorial process - First American Cancer Society Award Lecture on Cancer Epidemiology and Prevention. Cancer Res. 52:6735-6740. (Pubitemid 23006271)
10. Cover TL, Blanke SR. 2005. Helicobacter pylori VacA, a paradigm for toxin multifunctionality. Nat. Rev. Microbiol. 3:320-332. (Pubitemid 40443725)
11. Cover TL, Blaser MJ. 1992. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 267:10570-10575.
12. Cover TL, Puryear W, Perez-Perez GI, Blaser MJ. 1991. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect. Immun. 59:1264-1270.
13. Dixon MF, Genta RM, Yardley JH, Correa P. 1996. Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am. J. Surg. Pathol. 20:1161-1181.
14. Douraghi M, et al. 2009. Multiple gene status in Helicobacter pylori strains and risk of gastric cancer development. Digestion 80:200-207.
15. El-Omar EM, et al. 2001. The role of interleukin-1 polymorphisms in the pathogenesis of gastric cancer. Nature 412:99.
16. Ferreira RM, et al. 2012. Helicobacter pylori vacA intermediate region genotyping and progression of gastric preneoplastic lesions. Am. J. Gastroenterol. 107:145-146.
17. Figueiredo C, et al. 2002. Helicobacter pylori and interleukin 1 genotyping: an opportunity to identify high-risk individuals for gastric carcinoma. J. Natl. Cancer Inst. 94:1680-1687. (Pubitemid 35446836)
18. Gonzalez CA, et al. 2011. Helicobacter pylori cagA and vacA genotypes as predictors of progression of gastric preneoplastic lesions: a long-term follow-up in a high-risk area in Spain. Am. J. Gastroenterol. 106:867-874.
19. Gonzalez CA, et al. 2010. Gastric cancer occurrence in preneoplastic lesions: a long-term follow-up in a high-risk area in Spain. Int. J. Cancer 127:2654-2660.
20. Hussein NR, et al. 2008. Differences in virulence markers between Helicobacter pylori strains from Iraq and those from Iran: potential importance of regional differences in H. pylori-associated disease. J. Clin. Microbiol. 46:1774-1779. (Pubitemid 351657228)
21. Jang S, et al. 2010. Epidemiological link between gastric disease and polymorphisms in VacA and CagA. J. Clin. Microbiol. 48:559-567.
22. Jones KR, et al. 2011. Polymorphisms in the intermediate region of VacA impact Helicobacter pylori-induced disease development. J. Clin. Microbiol. 49:101-110.
23. Kuipers EJ, Perez-Perez GI, Meuwissen SG, Blaser MJ. 1995. Helicobacter pylori and atrophic gastritis: importance of the cagA status. J. Natl. Cancer Inst. 87:1777-1780.
24. Lauren P. 1965. The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma. An attempt at a histoclinical classification. Acta Pathol. Microbiol. Scand. 64:31-49.
25. Letley DP, Atherton JC. 2000. Natural diversity in the N terminus of the mature vacuolating cytotoxin of Helicobacter pylori determines cytotoxin activity. J. Bacteriol. 182:3278-3280. (Pubitemid 30326947)
26. Letley DP, Rhead JL, Twells RJ, Dove B, Atherton JC. 2003. Determinants of non-toxicity in the gastric pathogen Helicobacter pylori. J. Biol. Chem. 278:26734-26741. (Pubitemid 36876820)
27. Machado JC, et al. 2003. A proinflammatory genetic profile increases the risk for chronic atrophic gastritis and gastric carcinoma. Gastroenterology 125:364-371. (Pubitemid 36929416)
28. Machado JC, et al. 2001. Interleukin 1B and interleukin 1RN polymorphisms are associated with increased risk of gastric carcinoma. Gastroenterology 121:823-829. (Pubitemid 32959393)
29. Nogueira C, et al. 2001. Helicobacter pylori genotypes may determine gastric histopathology. Am. J. Pathol. 158:647-654.
30. Odenbreit S, et al. 2000. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 287:1497-1500. (Pubitemid 30127145)
31. Ogiwara H, et al. 2009. Role of deletion located between the intermediate and middle regions of the Helicobacter pylori vacA gene in cases of gastroduodenal diseases. J. Clin. Microbiol. 47:3493-3500.
32. Rhead JL, et al. 2007. A new Helicobacter pylori vacuolating cytotoxin determinant, the intermediate region, is associated with gastric cancer. Gastroenterology 133:926-936. (Pubitemid 47374139)
33. Scholte GH, et al. 2002. Genotyping of Helicobacter pylori in paraffin-embedded gastric biopsy specimens: relation to histological parameters and effects on therapy. Am. J. Gastroenterol. 97:1687-1695. (Pubitemid 34761390)
34. Scholte GH, van Doorn LJ, Quint WG, Linderman J. 2001. Genotyping of Helicobacter pylori strains in formalin-fixed or formaldehydesublimate-fixed paraffin-embedded gastric biopsy specimens. Diagn. Mol. Pathol. 10:166-170. (Pubitemid 32808111)
35. van Doorn LJ, et al. 1998. Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization. J. Clin. Microbiol. 36:1271-1276.
36. van Doorn LJ, et al. 1998. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology 115:58-66.