Albumin 3′untranslated region facilitates increased recombinant protein production from Chinese hamster ovary cells

Biotechnology Journal

Volumn 7, Issue 11, 2012, Pages 1405-1411

  Pearson, Mark J ^a   Khazaipoul, Siavash ^a   Optun, Asta ^b   Pryme, Ian F ^b,c   Stern, Beate ^b,c   Hesketh, John E ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b UniTargetingResearch AS   (Norway)

^c UNIVERSITY OF BERGEN   (Norway)

Author keywords

3 UTR; Cell factory; Expression vector; Luciferase; Reporter gene

Indexed keywords

CHINESE HAMSTER OVARY CELLS; CHO CELL; CODING SEQUENCES; CULTURE MEDIUM; DELETION ANALYSIS; EXPRESSION LEVELS; EXPRESSION VECTORS; LUCIFERASE; LUCIFERASE ACTIVITIES; MRNA LEVEL; PHARMACEUTICAL INDUSTRY; PROTEIN EXPRESSIONS; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS; REPORTER GENE; STEM-LOOP REGIONS; UNTRANSLATED REGIONS;

CELL CULTURE; CELLS; GENETIC ENGINEERING; RECOMBINANT PROTEINS;

GENE EXPRESSION;

ALBUMIN; CHYMOTRYPSINOGEN; IMMUNOGLOBULIN; LUCIFERASE; MESSENGER RNA; NUCLEOTIDE; RECOMBINANT PROTEIN; ALBUMINOID;

3' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; DRUG INDUSTRY; EXPRESSION VECTOR; GENE SEQUENCE; GENETIC CODE; MARINE SPECIES; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; PROTEIN SECRETION; ANIMAL; BIOTECHNOLOGY; CHEMISTRY; CONFORMATION; CRICETULUS; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

CRICETULUS GRISEUS; GAUSSIA PRINCEPS (T. SCOTT, 1894);

3' UNTRANSLATED REGIONS; ALBUMINS; ANIMALS; BASE SEQUENCE; BIOTECHNOLOGY; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; HUMANS; LUCIFERASES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RECOMBINANT PROTEINS; RNA, MESSENGER;

EID: 84868654833     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200044     Document Type: Article

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