Removing rRNA from deproteinized, phenol-extracted total RNA by enzymatic digestion

Cold Spring Harbor Protocols

Volumn 7, Issue 11, 2012, Pages 1187-1188

  Nilsen, Timothy W ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

EXONUCLEASE; RIBOSOME RNA; PHENOL; RIBONUCLEASE;

ARTICLE; ENZYMATIC ASSAY; RNA EXTRACTION; RNA PURIFICATION; SEDIMENTATION; HYDROLYSIS; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY;

HYDROLYSIS; MOLECULAR BIOLOGY; PHENOL; RIBONUCLEASES; RNA, RIBOSOMAL;

EID: 84868527998     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot072124     Document Type: Article

References (3)

1. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010a. Purification of RNA by SDS solubilization and phenol extraction. Cold Spring Harb Protoc doi:10.1101/pdb.prot5438.
2. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010b. Ethanol precipitation of RNA and the use of carriers. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5440.
3. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010c. Removal of ribosomal subunits (and rRNA) from cytoplasmic extracts before solubilization with SDS and deproteinization. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5442.