Establishment of tetracycline-inducible, survivin-expressing CHO cell lines by an optimized screening method

Bioscience, Biotechnology and Biochemistry

Volumn 76, Issue 10, 2012, Pages 1909-1912

  Zhao, Xinghui ^a   Yu, Yingqun ^a,b   Zhao, Zhanzhong ^c   Guo, Junwei ^a   Fu, Ling ^a   Yu, Ting ^a   Hou, Lihua ^a   Yi, Shaoqiong ^a   Chen, Wei ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

^b BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Apoptosis; Chinese hamster ovary cell; Enhanced green fluorescence protein; Survivin; Tetracycline inducible expression

Indexed keywords

ANNEXINS; ANTI-APOPTOSIS; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; DNA LADDERING; ENHANCED GREEN FLUORESCENCE PROTEINS; EXPRESSION SYSTEM; REPORTER GENE; SCREENING METHODS; STABLE CELL LINES; STABLE CLONES; STAINING ASSAYS; STRINGENT REGULATIONS; SURVIVIN; TETRACYCLINE-INDUCIBLE EXPRESSION;

CELL DEATH; CLONING; CLOUDS; GENES; OPTIMIZATION;

CELL CULTURE;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; INHIBITOR OF APOPTOSIS PROTEIN; REPRESSOR PROTEIN; TETRACYCLINE; TETRACYCLINE RESISTANCE ENCODING TRANSPOSON REPRESSOR PROTEIN; TETRACYCLINE RESISTANCE-ENCODING TRANSPOSON REPRESSOR PROTEIN;

ANIMAL; ARTICLE; CHO CELL; CRICETULUS; DRUG EFFECT; GENE EXPRESSION; GENETIC PROCEDURES; GENETICS; HAMSTER; REPORTER GENE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION; GENES, REPORTER; GENETIC TECHNIQUES; GREEN FLUORESCENT PROTEINS; INHIBITOR OF APOPTOSIS PROTEINS; REPRESSOR PROTEINS; TETRACYCLINE;

CRICETULUS GRISEUS;

EID: 84868525058     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.120395     Document Type: Article

References (11)

1. Gossen M and Bujard H, Proc. Natl. Acad. Sci. USA, 89, 5547-5551 (1992).
2. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, and Bujard H, Science, 268, 1766-1769 (1995).
3. Yao F, Svensjo T, Winkler T, Lu M, Eriksson C, and Eriksson E, Hum. Gene Ther., 9, 1939-1950 (1998).
4. Postle K, Nguyen TT, and Bertrand KP, Nucleic Acids Res., 12, 4849-4863 (1984).
5. Hillen W and Berens C, Annu. Rev. Microbiol., 48, 345-369 (1994).
6. Cormack BP, Valdivia RH, and Falkow S, Gene, 173, 33-38 (1996).
7. Chudakov DM, Matz MV, Lukyanov S, and Lukyanov KA, Physiol. Rev., 90, 1103-1163 (2010).
8. Zhao X, Guo J, Yu Y, Yi S, Yu T, Fu L, Hou L, and Chen W, Biotechnol. Lett., 33, 1293-1300 (2011).
9. Lecoeur H, Ledru E, Prevost MC, and Gougeon ML, J. Immunol. Methods, 209, 111-123 (1997).
10. Srinivasula SM and Ashwell JD, Mol. Cell, 30, 123-135 (2008).
11. Altieri DC, Nat. Rev. Cancer, 8, 61-70 (2008).