Effect of immunization with a recombinant cholera toxin B subunit/somatostatin fusion protein on immune response and growth hormone levels in mice

Biotechnology Letters

Volumn 34, Issue 12, 2012, Pages 2199-2203

  Wu, Bingbing ^a   Yuan, Taoyan ^a   Qi, Ruili ^a   He, Jun ^a   Fu, Yan ^a   Niu, Dong ^a   Li, Weifen ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Cholera toxin B subunit; Growth hormone; Immune response; Mice; Somatostatin

Indexed keywords

CHOLERA TOXIN B SUBUNIT; GROWTH HORMONES; IMMUNE RESPONSE; MICE; SOMATOSTATIN;

ESCHERICHIA COLI; IMMUNIZATION; IMMUNOLOGY; MAMMALS;

ENZYME INHIBITION;

CHOLERA TOXIN; GROWTH HORMONE; HYBRID PROTEIN; IMMUNOLOGICAL ADJUVANT; RECOMBINANT VACCINE; SOMATOSTATIN;

ANIMAL; ARTICLE; BLOOD; DRUG ANTAGONISM; ESCHERICHIA COLI; GENETICS; IMMUNIZATION; IMMUNOLOGY; METHODOLOGY; MOUSE;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; CHOLERA TOXIN; ESCHERICHIA COLI; GROWTH HORMONE; IMMUNIZATION; MICE; RECOMBINANT FUSION PROTEINS; SOMATOSTATIN; VACCINES, SYNTHETIC;

ESCHERICHIA COLI; MUS;

EID: 84868499636     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-1027-z     Document Type: Article

References (11)

1. Bai LY, Liang AX, Zhang J, Yang FF, Han L, Huo LJ, Yang LG (2011) Effects of immunization against a DNA vaccine encoding somatostatin gene (pGM-CSF/SS) by attenuated Salmonella typhimurium on growth, reproduction and lactation in female mice. Theriogenology 75: 155-163.
2. Cavallaro AS, Mahony D, Commins M, Mahony TJ, Mitter N (2011) Endotoxin-free purification for the isolation of bovine viral diarrhoea virus E2 protein from insoluble inclusion body aggregates. Microb Cell Fact 10: 57.
3. Gong Z, Jin Y, Zhang Y (2005) Oral administration of a cholera toxin B subunit-insulin fusion protein produced in silkworm protects against autoimmune diabetes. J Biotechnol 119: 93-105.
4. Harakuni T, Sugawa H, Komesu A, Tadano M, Arakawa T (2005) Heteropentameric cholera toxin B subunit chimeric molecules genetically fused to a vaccine antigen induce systemic and mucosal immune responses: a potential new strategy to target recombinant vaccine antigens to mucosal immune systems. Infect Immun 73: 5654-5665.
5. Holmgren J, Lycke N, Czerkinsky C (1993) Cholera toxin and cholera B subunit as oral-mucosal adjuvant and antigen vector systems. Vaccine 11: 1179-1184.
6. Liljeqvist S, Ståhl S, Andréoni C, Binz H, Uhlén M, Murby M (1997) Fusions to the cholera toxin B subunit: influence on pentamerization and GM1 binding. J Immunol Methods 210: 125-135.
7. Rodríguez-Carmona E, Cano-Garrido O, Seras-Franzoso J, Villaverde A, García-Fruitós E (2010) Isolation of cell-free bacterial inclusion bodies. Microb Cell Fact 9: 71.
8. Roy S, Tyagi A, Tiwari S, Singh A, Sawant SV, Singh PK, Tuli R (2010) Rabies glycoprotein fused with B subunit of cholera toxin expressed in tobacco plants folds into biologically active pentameric protein. Protein Expr Purif 70: 184-190.
9. Weckbecker G, Lewis I, Albert R, Schmid HA, Hoyer D, Bruns C (2003) Opportunities in somatostatin research: biological, chemical and therapeutic aspects. Nat Rev Drug Discov 2: 999-1017.
10. Wu B, Qi R, Li B, Yuan T, Liu H, He J, Lin Z, Li W, Fu Y, Niu D (2012) Effect of active immunization against a recombinant mouse granulocyte-macrophage colony-stimulating factor/somatostatin fusion protein on the growth of mice. Mol Biol Rep 39: 6773-6779.
11. Xue CL, Liang AX, Mao DG, Han L, Liu XB, Zhang J, Yang LG (2010) Effect of genetic adjuvants on immune respondance, growth and hormone levels in somatostatin DNA vaccination-induced Hu lambs. Vaccine 28: 1541-1546.