The HIV-1 Rev Protein Enhances Encapsidation of Unspliced and Spliced, RRE-Containing Lentiviral Vector RNA

PLoS ONE

Volumn 7, Issue 11, 2012, Pages

  Grewe, Bastian ^a   Ehrhardt, Katrin ^a,b   Hoffmann, Bianca ^a   Blissenbach, Maik ^a   Brandt, Sabine ^a,c   Überla, Klaus ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c Medical Laboratory Bremen   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GAG PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; REV PROTEIN; TRANSACTIVATOR PROTEIN; VIRUS RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; BINDING SITE; CONTROLLED STUDY; EXON; GENE SEQUENCE; GROUP SPECIFIC ANTIGEN GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRE GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE GENE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; REV GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SPLICING; SEQUENCE ANALYSIS; STRUCTURAL GENE; TAT GENE; UPREGULATION; VIRION; VIRUS CAPSID; VIRUS GENE; VIRUS GENOME; VIRUS PARTICLE; WESTERN BLOTTING; WILD TYPE;

ACTIVE TRANSPORT, CELL NUCLEUS; ALTERNATIVE SPLICING; GENETIC VECTORS; GENOME, VIRAL; HEK293 CELLS; HIV-1; HUMANS; LENTIVIRUS; MUTATION; PLASMIDS; RESPONSE ELEMENTS; REV GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; RNA; RNA SPLICING; RNA, VIRAL; TRANSFECTION;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84868311398     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048688     Document Type: Article

References (39)

1. D'Souza V, Summers MF, (2005) How retroviruses select their genomes. Nat Rev Microbiol 3: 643-655.
2. Purcell DF, Martin MA, (1993) Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J Virol 67: 6365-6378.
3. Stoltzfus CM, Madsen JM, (2006) Role of viral splicing elements and cellular RNA binding proteins in regulation of HIV-1 alternative RNA splicing. Curr HIV Res 4: 43-55.
4. Bohne J, Wodrich H, Krausslich HG, (2005) Splicing of human immunodeficiency virus RNA is position-dependent suggesting sequential removal of introns from the 5′ end. Nucleic Acids Res 33: 825-837.
5. Lever AML (1999) Lentiviral Vectors. In: Meager A, editor. Gene Therapy Technologies, Applications and Regulations. Chichester, West Sussex, England: John Wiley & Sons Ltd. 61-86.
6. Saurya S, Lichtenstein Z, Karpas A, (2005) Defective rev response element (RRE) and rev gene in HAART treated AIDS patients with discordance between viral load and CD4+ T-cell counts. J Clin Virol 33: 324-327.
7. Geigenmuller U, Linial ML, (1996) Specific binding of human immunodeficiency virus type 1 (HIV-1) Gag-derived proteins to a 5′ HIV-1 genomic RNA sequence. J Virol 70: 667-671.
8. Didierlaurent L, Racine PJ, Houzet L, Chamontin C, Berkhout B, et al. (2011) Role of HIV-1 RNA and protein determinants for the selective packaging of spliced and unspliced viral RNA and host U6 and 7SL RNA in virus particles. Nucleic Acids Res 39: 8915-8927.
9. Houzet L, Paillart JC, Smagulova F, Maurel S, Morichaud Z, et al. (2007) HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms. Nucleic Acids Res 35: 2695-2704.
10. Houzet L, Morichaud Z, Mougel M, (2007) Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells. Retrovirology 4: 30.
11. Liang C, Hu J, Russell RS, Kameoka M, Wainberg MA, (2004) Spliced human immunodeficiency virus type 1 RNA is reverse transcribed into cDNA within infected cells. AIDS Res Hum Retroviruses 20: 203-211.
12. Blissenbach M, Grewe B, Hoffmann B, Brandt S, Uberla K, (2010) Nuclear RNA export and packaging functions of HIV-1 Rev revisited. J Virol 84: 6598-6604.
13. Brandt S, Blissenbach M, Grewe B, Konietzny R, Grunwald T, et al. (2007) Rev proteins of human and simian immunodeficiency virus enhance RNA encapsidation. PLoS Pathog 3: e54.
14. Grewe B, Überla K (2010) Rev Revisited: Additional Functions of the HIV-1 Rev Protein. In: Lever AML, Jeang K-T, Berkhout B, editors. Recent Adcances in Human Retroviruses: Principles of Replication and Pathogenesis. Singapore: World Scientific Publishing Co. Pte. Ltd. 439-470.
15. Cockrell AS, van Praag H, Santistevan N, Ma H, Kafri T, (2011) The HIV-1 Rev/RRE system is required for HIV-1 5′ UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles. Retrovirology 8: 51.
16. Lucke S, Grunwald T, Uberla K, (2005) Reduced mobilization of Rev-responsive element-deficient lentiviral vectors. J Virol 79: 9359-9362.
17. Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM, (1998) Development of a self-inactivating lentivirus vector. J Virol 72: 8150-8157.
18. Grewe B, Hoffmann B, Ohs I, Blissenbach M, Brandt S, et al. (2012) Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with Gag. J Virol 86: 2990-3002.
19. Galla M, Will E, Kraunus J, Chen L, Baum C, (2004) Retroviral pseudotransduction for targeted cell manipulation. Mol Cell 16: 309-315.
20. Haas DL, Case SS, Crooks GM, Kohn DB, (2000) Critical factors influencing stable transduction of human CD34(+) cells with HIV-1-derived lentiviral vectors. Mol Ther 2: 71-80.
21. Kim SS, Kothari N, You XJ, Robinson WE Jr, Schnell T, et al. (2001) Generation of replication-defective helper-free vectors based on simian immunodeficiency virus. Virology 282: 154-167.
22. Anson DS, Fuller M, (2003) Rational development of a HIV-1 gene therapy vector. J Gene Med 5: 829-838.
23. Cui Y, Iwakuma T, Chang LJ, (1999) Contributions of viral splice sites and cis-regulatory elements to lentivirus vector function. J Virol 73: 6171-6176.
24. Kotsopoulou E, Kim VN, Kingsman AJ, Kingsman SM, Mitrophanous KA, (2000) A Rev-independent human immunodeficiency virus type 1 (HIV-1)-based vector that exploits a codon-optimized HIV-1 gag-pol gene. J Virol 74: 4839-4852.
25. Mautino MR, Ramsey WJ, Reiser J, Morgan RA, (2000) Modified human immunodeficiency virus-based lentiviral vectors display decreased sensitivity to trans-dominant Rev. Hum Gene Ther 11: 895-908.
26. Graf M, Bojak A, Deml L, Bieler K, Wolf H, et al. (2000) Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression. J Virol 74: 10822-10826.
27. Seguin B, Staffa A, Cochrane A, (1998) Control of human immunodeficiency virus type 1 RNA metabolism: role of splice sites and intron sequences in unspliced viral RNA subcellular distribution. J Virol 72: 9503-9513.
28. Greatorex JS, Palmer EA, Pomerantz RJ, Dangerfield JA, Lever AM, (2006) Mutation of the Rev-binding loop in the human immunodeficiency virus 1 leader causes a replication defect characterized by altered RNA trafficking and packaging. J Gen Virol 87: 3039-3044.
29. Houzet L, Morichaud Z, Didierlaurent L, Muriaux D, Darlix JL, et al. (2008) Nucleocapsid mutations turn HIV-1 into a DNA-containing virus. Nucleic Acids Res 36: 2311-2319.
30. Luban J, Goff SP, (1994) Mutational analysis of cis-acting packaging signals in human immunodeficiency virus type 1 RNA. J Virol 68: 3784-3793.
31. Bagnarelli P, Valenza A, Menzo S, Sampaolesi R, Varaldo PE, et al. (1996) Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo. J Virol 70: 7603-7613.
32. Butera ST, Roberts BD, Lam L, Hodge T, Folks TM, (1994) Human immunodeficiency virus type 1 RNA expression by four chronically infected cell lines indicates multiple mechanisms of latency. J Virol 68: 2726-2730.
33. Peng H, Reinhart TA, Retzel EF, Staskus KA, Zupancic M, et al. (1995) Single cell transcript analysis of human immunodeficiency virus gene expression in the transition from latent to productive infection. Virology 206: 16-27.
34. Pomerantz RJ, Seshamma T, Trono D, (1992) Efficient replication of human immunodeficiency virus type 1 requires a threshold level of Rev: potential implications for latency. J Virol 66: 1809-1813.
35. Tange TO, Nott A, Moore MJ, (2004) The ever-increasing complexities of the exon junction complex. Curr Opin Cell Biol 16: 279-284.
36. Malim MH, Hauber J, Fenrick R, Cullen BR, (1988) Immunodeficiency virus rev trans-activator modulates the expression of the viral regulatory genes. Nature 335: 181-183.
37. Fouchier RA, Meyer BE, Simon JH, Fischer U, Malim MH, (1997) HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J 16: 4531-4539.
38. Wagner R, Graf M, Bieler K, Wolf H, Grunwald T, et al. (2000) Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: implications for the safety of lentiviral vectors. Hum Gene Ther 11: 2403-2413.
39. Schnell T, Foley P, Wirth M, Munch J, Uberla K, (2000) Development of a self-inactivating, minimal lentivirus vector based on simian immunodeficiency virus. Hum Gene Ther 11: 439-447.