Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing

Virology Journal

Volumn 9, Issue , 2012, Pages

  Li, Kelvin ^a   Shrivastava, Susmita ^a   Brownley, Anushka ^b   Katzel, Dan ^a   Bera, Jayati ^a   Nguyen, Anh Thu ^c   Thovarai, Vishal ^a   Halpin, Rebecca ^a   Stockwell, Timothy B ^a  

^a J CRAIG VENTER INSTITUTE   (United States)

^b BioTeam Inc   (United States)

^c UNIVERSITY OF VIRGINIA   (United States)

Author keywords

High throughput computational degenerate PCR primer design; sequencing viral isolates

Indexed keywords

ARTICLE; CONSENSUS SEQUENCE; GENE AMPLIFICATION; GENETIC VARIABILITY; HIGH THROUGHPUT SEQUENCING; MEASLES; MEASLES VIRUS; METAPNEUMOVIRUS; MUMPS VIRUS; NONHUMAN; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 3; POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTION; RUBELLA VIRUS; VIRUS GENOME; VIRUS ISOLATION;

DNA PRIMERS; GENOME, VIRAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; POLYMERASE CHAIN REACTION; VIRUSES;

HUMAN METAPNEUMOVIRUS; HUMAN PARAINFLUENZA VIRUS 1; HUMAN RESPIRATORY SYNCYTIAL VIRUS A; MEASLES VIRUS; MUMPS VIRUS;

EID: 84868270276     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-9-261     Document Type: Article

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