Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 10, 2012, Pages 1689-1696

  Thiery, Gwendoline ^a,b,c   Mernaugh, Ray L ^b   Yan, Heping ^b,d   Spraggins, Jeffrey M ^a,b   Yang, Junhai ^a,b   Parl, Fritz F ^b   Caprioli, Richard M ^a,b  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Imaging mass spectrometry; Immunohistochemistry; Laser desorption ionization mass spectrometry; Single chain fragment variable recombinant antibodies; Targeted multiplex imaging mass spectrometry

Indexed keywords

HIGH SENSITIVITY; HUMAN BREAST CANCER; IMAGING MASS SPECTROMETRY; IMMUNOHISTOCHEMISTRY; LASER DESORPTION IONIZATION MASS SPECTROMETRY; RECOMBINANT ANTIBODY; REPORTER MOLECULES; SINGLE CHAINS; TARGETED IMAGING; TECHNICAL BARRIERS; TISSUE SAMPLES;

ANTIGENS; ENZYMES; MULTIPLEXING; PARAFFINS; SECONDARY ION MASS SPECTROMETRY; TISSUE;

ANTIBODIES;

AVIDIN; CYTOCHROME P450 1A1; CYTOCHROME P450 1B1; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANALYTIC METHOD; ANIMAL TISSUE; ANTIBODY LABELING; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; BREAST CANCER; CANCER TISSUE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMAGE ANALYSIS; MASS SPECTROMETRY; MASS SPECTROMETRY IMAGING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR IMAGING; MOUSE; NONHUMAN; PERFORMANCE MEASUREMENT SYSTEM; RAT; SENSITIVITY AND SPECIFICITY; TISSUE DISTRIBUTION;

ANIMALS; ANTIBODIES, MONOCLONAL; ARYL HYDROCARBON HYDROXYLASES; AVIDIN; BIOTIN; BREAST; BREAST NEOPLASMS; CYTOCHROME P-450 CYP1A1; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HISTOCYTOCHEMISTRY; HUMANS; MASS SPECTROMETRY; MICE; MOLECULAR IMAGING; RATS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SINGLE-CHAIN ANTIBODIES;

EID: 84868246683     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0423-x     Document Type: Article

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