Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles

Journal of Biomolecular NMR

Volumn 54, Issue 2, 2012, Pages 129-133

  Stanczak, Pawel ^a   Zhang, Qinghai ^a   Horst, Reto ^a   Serrano, Pedro ^a   Wüthrich, Kurt ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

E. coli outer membrane protein W; Integral membrane proteins; Solubilization in detergent micelles; Structural biology

Indexed keywords

DETERGENT; MEMBRANE PROTEIN; PHOSPHORYLCHOLINE; PROTEIN OMPW; TIOCONAZOLE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DEUTERON NUCLEAR MAGNETIC RESONANCE; MEMBRANE RECONSTITUTION; MICELLE; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROCESS MONITORING; PROCESS OPTIMIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; TEMPERATURE;

BACTERIAL OUTER MEMBRANE PROTEINS; DETERGENTS; DEUTERIUM; ESCHERICHIA COLI PROTEINS; MICELLES; PHOSPHORYLCHOLINE; PROTEIN CONFORMATION; PROTEIN STABILITY;

EID: 84868212175     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-012-9658-x     Document Type: Article

References (20)

1. Arora A, Abildgaard F, Bushweller JH, Tamm LK (2001) Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nat Struct Biol 8:334-338 (Pubitemid 32275019)
2. Bartels C, Xia T, Billeter M, Güntert P, Wüthrich K (1995) The program XEASY for computer-supported NMR spectral analysis of biological macromolecules. J Biomol NMR 6:1-10
3. Bill RM, Henderson PJ, Iwata S, Kunji ER, Michael H, Neutze R, Newstead S, Poolman B, Tate CG, Vogel H (2011) Overcoming barriers to membrane protein structure determination. Nat Biotechnol 29:335-340
4. Busch W, Saier MH Jr (2003) The IUBMB-endorsed transporter classification system. Methods Mol Biol 227:21-36
5. DeMarco A, Wüthrich K (1976) Digital filtering with a sinusoidal window function: An alternative technique for resolution enhancement in FT NMR. J Magn Reson 24:201-204
6. Fairman JW, Noinaj N, Buchanan SK (2011) The structural biology of b-barrel membrane proteins: A summary of recent reports. Curr Opin Struct Biol 21:523-531
7. Fernández C, Wüthrich K (2003) NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett 555:144-150 (Pubitemid 37431112)
8. Fernández C, Hilty C, Bonjour S, Adeishvili K, Pervushin K, Wüthrich K (2001) Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli. FEBS Lett 504:173-178 (Pubitemid 32787066)
9. Fernández C, Hilty C, Wider G, Güntert P, Wüthrich K (2004) NMR structure of the integral membrane protein OmpX. J Mol Biol 336:1211-1221 (Pubitemid 38229708)
10. Hong H, Patel DR, Tamm LK, van den Berg B (2006) The outer membrane protein OmpW forms an eight-stranded b-barrel with a hydrophobic channel. J Biol Chem 28:7568-7577 (Pubitemid 43847527)
11. Horst R, Horwich AL, Wüthrich K (2011) Translational diffusion of macromolecular assemblies measured using transverse-relaxation-optimized pulse field gradient NMR. J Am Chem Soc 133:16354-16357
12. Horst R, Stanczak P, Serrano P, Wüthrich K (2012) Translational diffusion measurements by micro-coil NMR in aqueous solutions of the Fos-10 detergent-solubilized membrane protein OmpX. J Phys Chem B 116:6775-6780
13. Jalajakumari MB, Manning PA (1990) Nucleotide sequence of the gene, OmpW, encoding a 22 kDa immunogenic outer membrane protein of Vibrio cholera. Nucl Acids Res 18:2180 (Pubitemid 20171556)
14. Lee D, Hilty C, Wider G, Wüthrich K (2006) Efficient correlation time measurements for macromolecules from NMR relaxation interference. J Magn Reson 178:72-76 (Pubitemid 41774013)
15. Pautsch A, Schulz GE (1998) Structure of the outer membrane protein A transmembrane domain. Nat Struct Biol 5:1013-1017 (Pubitemid 28506635)
16. Pautsch A, Schulz GE (2000) High-resolution structure of the OmpA membrane domain. J Mol Biol 298:273-282
17. Pervushin K, Riek R, Wider G, Wüthrich K (1997) Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci USA 94:12366-12371
18. Stanczak P, Horst R, Serrano P, Wüthrich K (2009) NMR characterization of membrane protein-detergent micelle solutions by use of microcoil equipment. J Am Chem Soc 131:18450-18456
19. Vogt J, Schulz GE (1999) The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence. Structure 7:1301-1309 (Pubitemid 29482991)
20. Zhang Q, Horst R, Geralt M, Ma X, Hong W, Finn MG, Stevens RC, Wüthrich K (2008) Microscale NMR screening of new detergents for membrane protein structural biology. J Am Chem Soc 130:7357-7363 (Pubitemid 351813228)