Optimization of a direct spectrophotometric method to investigate the kinetics and inhibition of sialidases

BMC Biochemistry

Volumn 13, Issue 1, 2012, Pages

  Hayre, Jasvinder Kaur ^a   Xu, Guogang ^b,c   Borgianni, Luisa ^a   Taylor, Garry L ^d   Andrew, Peter W ^e   Docquier, Jean Denis ^a   Oggioni, Marco R ^a  

^a UNIVERSITY OF SIENA   (Italy)

^b NANCHANG UNIVERSITY   (China)

^c CHINESE PLA GENERAL HOSPITAL   (China)

^d UNIVERSITY OF ST ANDREWS   (United Kingdom)

^e UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Chromogenic sialic acids; Kinetic assay; Neuraminidase; Sialidase; Streptococcus pneumoniae

Indexed keywords

BACTERIAL ENZYME; NANA PROTEIN; NANB PROTEIN; NANC PROTEIN; NEURAMINIC ACID DERIVATIVE; SIALIDASE; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BIOCATALYSIS; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; REACTION TIME; SPECTROPHOTOMETRY; STREPTOCOCCUS PNEUMONIAE;

BIOCATALYSIS; BUFFERS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; STREPTOCOCCUS PNEUMONIAE; SUBSTRATE SPECIFICITY;

STREPTOCOCCUS PNEUMONIAE;

EID: 84868207821     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-13-19     Document Type: Article

References (22)

1. Sialidases: structures, biological significance and therapeutic potential. Taylor GL, Curr Opin Struct Biol 1996 6 830 837 10.1016/S0959- 440X(96)80014-5 8994884 (Pubitemid 27007669)
2. The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism. Luo Y, Li SC, Li YT, Luo M, J Mol Biol 1999 285 323 332 10.1006/jmbi.1998.2345 9878409 (Pubitemid 29034047)
3. NMR spectroscopic and molecular modeling investigations of the trans-sialidase from Trypanosoma cruzi. Haselhorst T, Wilson JC, Liakatos A, Kiefel MJ, Dayson JC, Glycobiology 2004 14 895 907 10.1093/glycob/cwh108 15190004 (Pubitemid 39272801)
4. Variation in the presence of neuraminidase genes among Streptococcus pneumoniae isolates with identical sequence types. Pettigrew MM, Fennie KP, York MP, Daniels J, Ghaffar F, Infect Immun 2006 74 3360 3365 10.1128/IAI.01442-05 16714565 (Pubitemid 43794525)
5. Crystal structure of the NanB sialidase from Streptococcus pneumoniae. Xu G, Potter JA, Russell RJ, Oggioni MR, Andrew PW, Taylor GL, J Mol Biol 2008 384 436 449 10.1016/j.jmb.2008.09.032 18835278
6. Three Streptococcus pneumoniae sialidases: three different products. Xu G, Kiefel MJ, Wilson JC, Andrew PW, Oggioni MR, Taylor GL, J Am Chem Soc 2011 133 1718 1721 10.1021/ja110733q 21244006
7. Structural and functional studies of Streptococcus pneumoniae neuraminidase B: An intramolecular trans-sialidase. Gut H, King SJ, Walsh MA, FEBS Lett 2008 582 3348 3352 10.1016/j.febslet.2008.08.026 18775704
8. The thiobarbituric acid assay of sialic acids. Warren L, J Biol Chem 1959 234 1971 1975 13672998
9. Cloning and expression of sialidase L, a NeuAcalpha2→3Gal-specific sialidase from the leech, Macrobdella decora. Chou MY, Li SC, Li YT, J Biol Chem 1996 271 19219 19224 10.1074/jbc.271.32.19219 8702601 (Pubitemid 26271586)
10. Properties of recombinant human cytosolic sialidase HsNEU2. The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules. Trignali C, Papini N, Fusi P, Croci G, Borsani G, Preti A, Tortora P, Tettamanti G, Venerando B, Monti E, J Biol Chem 2004 279 3169 3179 14613940 (Pubitemid 38140549)
11. Recent advances in the synthesis of sialic acid derivatives and sialylmimetics as biological probes. Kiefel MJ, von Itzstein M, Chem Rev 2002 102 471 490 10.1021/cr000414a 11841251 (Pubitemid 35377622)
12. The synthesis of 4-methylumbelliferyl alpha-ketoside of N-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase. Myers RW, Lee RT, Lee YC, Thomas GH, Reynolds LW, Uchida Y, Anal Biochem 1980 101 166 174 10.1016/0003-2697(80)90056-1 6243885 (Pubitemid 10136707)
13. Phase variable desialylation of host proteins that bind to Streptococcus pneumoniae in vivo and protect the airway. King SJ, Hippe KR, Gould JM, Bae D, Peterson S, Cline RT, Fasching C, Janoff EM, Weiser JN, Mol Microbiol 2004 54 159 171 10.1111/j.1365-2958.2004.04252.x 15458413 (Pubitemid 39315166)
14. Sialic acid: a preventable signal for pneumococcal biofilm, colonisation and invasion of the host. Trappetti C, Kadioglu A, Carter M, Athwal J, Iannelli F, Pozzi G, Andrew PW, Oggioni MR, J Infect Dis 2009 199 1497 1505 10.1086/598483 19392624
15. A functional genomics approach to establish the complement of carbohydrate transporters in Streptococcus pneumoniae. Bidossi A, Mulas L, Decorosi F, Colomba L, Ricci S, Pozzi G, Deutscher J, Viti C, Oggioni MR, PLoS One 2012 7 33320 10.1371/journal.pone.0033320 22428019
16. Regulation of neuraminidase expression in Streptococcus pneumoniae. Gualdi L, Kaur Hayre J, Gerlini A, Bidossi A, Colomba L, Trappetti C, Pozzi G, Docquier JD, Andrew PW, Ricci S, Oggioni MR, BMC Microbiol 2012 12 200 10.1186/1471-2180-12-200 22963456
17. Structure of the catalytic domain of Streptococcus pneumoniae sialidase NanA. Xu G, Andrew PW, Taylor GL, Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 64 772 775 10.1107/S1744309108024044 18765901
18. Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases. De Meester F, Joris B, Reckinger G, Bellefordis-Bourgignon C, Frere JM, Waley SG, Biochem Pharmacol 1987 36 2393 2403 10.1016/0006-2952(87)90609-5 3038122 (Pubitemid 17097419)
19. Structural basis for Streptococcus pneumoniae NanA inhibition by influenza antivirals zanamivir and oseltamivir carboxylate. Gut H, Xu G, Taylor GL, Walsh MA, J Mol Biol 2011 409 496 503 10.1016/j.jmb.2011.04.016 21514303
20. A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells. Wada T, Hata K, Yamaguchi K, Shiozaki K, Koseki S, Morya S, Miyagi T, Oncogene 2007 26 2483 2490 10.1038/sj.onc.1210341 17334392 (Pubitemid 46597274)
21. The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design. Russell RJ, Haire LF, Stevens DJ, Collins PJ, Lin YP, Blackburn GM, Hay AJ, Gamblin SJ, Skehel JJ, Nature 2006 443 45 49 10.1038/nature05114 16915235 (Pubitemid 44344043)
22. Structural analysis of a designed inhibitor complexed with the hemagglutinin-neuraminidase of Newcastle disease virus. Ryan C, Zaitsev V, Tindal DJ, Dayson JC, Thomson RJ, Alymova I, Porter A, von Itzstein M, Taylor GL, Glycoconj J 2006 23 135 141 10.1007/s10719-006-5446-8 16575531