Preferential Release of Newly Synthesized Insulin Assessed by a Multi-Label Reporter System Using Pancreatic β-Cell Line MIN6

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Hou, Ni ^a,b   Mogami, Hideo ^c,d   Kubota Murata, Chisato ^a   Sun, Meng ^a   Takeuchi, Toshiyuki ^a   Torii, Seiji ^a  

^a GUNMA UNIVERSITY   (Japan)

^b MEDICAL SCHOOL OF XI AN JIAOTONG UNIVERSITY   (China)

^c HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GLUCOSE; INSULIN; RED FLUORESCENT PROTEIN; TETRAMETHYLRHODAMINE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BIOCHEMISTRY; CELL STRAIN; CELL TRANSPORT; CELLULAR DISTRIBUTION; CHEMICAL LABELING; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; INSULIN RELEASE; INSULIN SYNTHESIS; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PULSE CHASE LABELING; SECRETORY GRANULE;

ANIMALS; BIOCHEMISTRY; CELL LINE; ENDOCRINE CELLS; FLUORESCENT DYES; GENES, REPORTER; GLUCOSE; INSULIN; INSULIN-SECRETING CELLS; INSULINOMA; MICE; MICROSCOPY, FLUORESCENCE; PLASMIDS; SECRETORY VESICLES;

BOVINAE;

EID: 84868156153     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0047921     Document Type: Article

References (21)

1. Dodson G, Steiner D, (1998) The role of assembly in insulin's biosynthesis. Curr Opin Struct Biol 8: 189-194.
2. Goodge KA, Hutton JC, (2000) Translational regulation of proinsulin biosynthesis and proinsulin conversion in the pancreatic beta-cell. Semin Cell Dev Biol 11: 235-242.
3. Eliasson L, Abdulkader F, Braun M, Galvanovskis J, Hoppa MB, et al. (2008) Novel aspects of the molecular mechanisms controlling insulin secretion. J Physiol 586: 3313-3324.
4. Sando H, Borg J, Steiner DF, (1972) Studies on the secretion of newly synthesized proinsulin and insulin from isolated rat islets of Langerhans. J Clin Invest 51: 1476-1485.
5. Gold G, Gishizky ML, Grodsky GM, (1982) Evidence that glucose "marks" beta cells resulting in preferential release of newly synthesized insulin. Science 218: 56-58.
6. Halban PA, (1982) Differential rates of release of newly synthesized and of stored insulin from pancreatic islets. Endocrinology 110: 1183-1188.
7. Rhodes CJ, Halban PA, (1987) Newly synthesized proinsulin/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway. J Cell Biol 105: 145-153.
8. Wang Z, Thurmond DC, (2009) Mechanisms of biphasic insulin-granule exocytosis- roles of the cytoskeleton, small GTPases and SNARE proteins. J Cell Sci 122: 893-903.
9. Michael DJ, Xiong W, Geng X, Drain P, Chow RH, (2007) Human insulin vesicle dynamics during pulsatile secretion. Diabetes 56: 1277-1288.
10. Svendsen S, Zimprich C, McDougall MG, Klaubert DH, Los GV, (2008) Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. BMC Cell Biol 9: 17.
11. Kasai K, Fujita T, Gomi H, Izumi T, (2008) Docking is not a prerequisite but a temporal constraint for fusion of secretory granules. Traffic 9: 1191-1203.
12. Torii S, Saito N, Kawano A, Hou N, Ueki K, et al. (2009) Gene silencing of phogrin unveils its essential role in glucose-responsive pancreatic beta-cell growth. Diabetes 58: 682-692.
13. Zhang B, Hosaka M, Sawada Y, Torii S, Mizutani S, et al. (2003) Parathyroid hormone-related protein induces insulin expression through activation of MAP kinase-specific phosphatase-1 that dephosphorylates c-Jun NH2-terminal kinase in pancreatic beta-cells. Diabetes 52: 2720-2730.
14. Kubota C, Torii S, Hou N, Saito N, Yoshimoto Y, et al. (2010) Constitutive reactive oxygen species generation from autophagosome/lysosome in neuronal oxidative toxicity. J Biol Chem 285: 667-674.
15. Saito N, Takeuchi T, Kawano A, Hosaka M, Hou N, et al. (2011) Luminal interaction of phogrin with carboxypeptidase E for effective targeting to secretory granules. Traffic 12: 499-506.
16. Yamaguchi K, Inoue S, Ohara O, Nagase T, (2009) Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins. Methods Mol Biol 577: 121-131.
17. Duncan RR, Greaves J, Wiegand UK, Matskevich I, Bodammer G, et al. (2003) Functional and spatial segregation of secretory vesicle pools according to vesicle age. Nature 422: 176-180.
18. Terskikh A, Fradkov A, Ermakova G, Zaraisky A, Tan P, et al. (2000) "Fluorescent timer": protein that changes color with time. Science 290: 1585-1588.
19. Ohara-Imaizumi M, Nakamichi Y, Tanaka T, Ishida H, Nagamatsu S, (2002) Imaging exocytosis of single insulin secretory granules with evanescent wave microscopy: distinct behavior of granule motion in biphasic insulin release. J Biol Chem 277: 3805-3808.
20. Ohara-Imaizumi M, Fujiwara T, Nakamichi Y, Okamura T, Akimoto Y, et al. (2007) Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis. J Cell Biol 177: 695-705.
21. Pedersen MG, Sherman A, (2009) Newcomer insulin secretory granules as a highly calcium-sensitive pool. Proc Natl Acad Sci U S A 106: 7432-7436.