Is Buffer a Good Proxy for a Crowded Cell-Like Environment? A Comparative NMR Study of Calmodulin Side-Chain Dynamics in Buffer and E. coli Lysate

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Latham, Michael P ^a   Kay, Lewis E ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LYSATE; BUFFER; CALCIUM ION; CALMODULIN; ISOLEUCINE; LEUCINE; METHIONINE; MYOSIN LIGHT CHAIN KINASE; VALINE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; CARBOXY TERMINAL SEQUENCE; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); DISPERSION; ESCHERICHIA COLI; GENE OVEREXPRESSION; IN VIVO STUDY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS;

ALGORITHMS; AMINO ACIDS; APOPROTEINS; BUFFERS; CALCIUM; CALMODULIN; CHELATING AGENTS; COMPUTER SIMULATION; EGTAZIC ACID; ESCHERICHIA COLI; MODELS, BIOLOGICAL; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SOLUTIONS;

EID: 84868122319     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048226     Document Type: Article

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