Identifying acetylated proteins in mitosis

Methods in Molecular Biology

Volumn 909, Issue , 2012, Pages 181-204

  Chuang, Carol ^a   Yu Lee, Li Yuan ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Acetylated proteins; Apicidin; HDAC3; Immunoprecipitation; Mass spectrometry; Mitosis; Nocodazole enrichment; Reduced lysate; Thymidine block

Indexed keywords

ACETYLATED PROTEIN; ANTISERUM; APICIDIN; HISTONE DEACETYLASE; HISTONE DEACETYLASE 3; MONOCLONAL ANTIBODY; PROTEIN; PROTEIN NUDC; UNCLASSIFIED DRUG;

ACETYLATION; ANTIBODY SPECIFICITY; ARTICLE; CELL SYNCHRONIZATION; ELECTROSPRAY MASS SPECTROMETRY; HELA CELL; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; MITOSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; WESTERN BLOTTING;

EID: 84868093656     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-959-4_13     Document Type: Article

References (20)

1. Ishii S, Kurasawa Y, Wong J et al (2008) Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-micro-tubule attachment. Proc Natl Acad Sci 105:4179-4184
2. Chuang C, Lin S-H, Huang F et al (2010) Acetylation of RNA processing proteins and cell cycle proteins in mitosis. J Proteome Res 9:4554-4564
3. Dephoure N, Zhou C, Villen J et al (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci 105:10762-10767
4. Malik R, Lenobel R, Santamaria A et al (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 10:4553-4563
5. Choudhary C, Kumar C, Gnad F et al (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325:834-840
6. Kim SC, Sprung R, Chen Y et al (2006) Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. Mol Cell 23:607-618
7. Kim GW and Yang XJ (2010) Comprehensive lysine acetylomes emerging from bacteria to humans. Trends Biochem Sci 36:211-220
8. Nishino M, Kurasawa Y, Evans R et al (2006) NudC is required for Plk1 targeting to the kinetochore and chromosome congression. Curr Biol 16:1414-1421
9. Josic D, Brown MK, Huang F et al (2005) Use of selective extraction and fast chromatographic separation combined with electrophoretic methods for mapping of membrane proteins. Electrophoresis 26:2809-2822
10. Huang F, Clifton J, Yang X et al (2009) SELDI-TOF as a method for biomarker discovery in the urine of aristolochic-acid-treated mice. Electrophoresis 30:1168-1174
11. Clifton JG, Huang F, Kovac S et al (2009) Proteomic characterization of plasma-derived clotting factor VIII-von Willebrand factor concentrates. Electrophoresis 30: 3636-3646
12. Rao PN and Engelberg J (1965) HeLa cells: Effects of temperature on the life cycle. Science 148:1092-1094
13. Morris NR and Fischer GA (1960) Studies concerning inhibition of the synthesis of deoxy-cytidine by phosphorylated derivatives of thy-midine. Biochim Biophys Acta 42:183-184
14. Xeros N (1962) Deoxyriboside control and synchronization of mitosis. Nature 194:682-683
15. Studzinski GP and Lambert WC (1969) Thymidine as a synchronizing agent. I. Nucleic acid and protein formation in synchronous HeLa cultures treated with excess thymidine. J Cell Physiol 73:109-117
16. Bostock CJ, Prescott DM, Kirkpatrick JB (1971) An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border. Exp Cell Res 68:163-168
17. Atassi G and Tagnon HJ (1975) R17934-NSC 238159: a new antitumor drug-I. Effect on experimental tumors and factors in fluencing effectiveness. Eur J Cancer 11:599-607
18. Khan N, Jeffers M, Kumar S et al (2008) Determination of the class and isoform selectivity of small molecule histone deacetylase inhibitors. Biochem J 409:581-589
19. Brinkley BR and Cartwright J Jr (1975) Cold-labile and cold-stable microtubules in the mitotic spindle of mammalian cells. Ann New York Acad Sci 253:428-439
20. Lin SH and Guidotti G (2009) Puri fication of membrane proteins. Methods Enzymol 463: 619-629