Metabolic Profiling Reveals Sphingosine-1-Phosphate Kinase 2 and Lyase as Key Targets of (Phyto-) Estrogen Action in the Breast Cancer Cell Line MCF-7 and Not in MCF-12A

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Engel, Nadja ^a   Lisec, Jan ^b   Piechulla, Birgit ^a   Nebe, Barbara ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PHYTOESTROGEN; SPHINGOSINE 1 PHOSPHATE; SPHINGOSINE 1 PHOSPHATE KINASE 2; SPHINGOSINE 1 PHOSPHATE LYASE; UNCLASSIFIED DRUG;

ACIDIFICATION; ANALYSIS OF VARIANCE; APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL ADHESION; CELL METABOLISM; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME REGULATION; EPITHELIUM CELL; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GLYCOLYSIS; HUMAN; HUMAN CELL; LIPID METABOLISM; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; UPREGULATION;

ALDEHYDE-LYASES; BREAST; BREAST NEOPLASMS; CELL LINE; CELL LINE, TUMOR; ESTRADIOL; FEMALE; GENISTEIN; HUMANS; METABOLOME; MOLECULAR TARGETED THERAPY; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PHYTOESTROGENS; SPHINGOSINE;

LINUM USITATISSIMUM;

EID: 84868087219     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0047833     Document Type: Article

References (32)

1. Makiewicz L, Garey J, Adlercreutz H, Gurpide E, (1993) In vitro bioassays of non-steroidal phytoestrogens. J Steroid Biochem Mol Biol 45: 399-405.
2. Hilakivi-Clarke L, Cabanes A, Olivo S, Kerr L, Bouker KB, et al. (2002) Do estrogens always increase breast cancer risk? J Steroid Biochem Mol Biol 80(2): 163-74.
3. Zava DT, Duwe G, (1997) Estrogenic and antiproliferative properties of genistein and other flavonoids in human breast cancer cells in vitro. Nutr Cancer 27: 31-40.
4. Allred CD, Allred KF, Ju YH, Virant SM, Helferich WG, (2001) Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose-dependent manner. Cancer Res 61: 5045-5050.
5. Pagliacci MC, Smacchia M, Migliorati G, Grignani F, Riccardi C, et al. (1994) Growth-inhibitory effects of the natural phyto-oestrogen genistein in MCF-7 human breast cancer cells. Eur J Cancer 30A: 1675-1682.
6. Satih S, Chalabi N, Rabiau N, Bosviel R, Fontana L, et al. (2010) Gene expression profiling of breast cancer cell lines in response to soy isoflavones using a pangenomic microarray approach. OMICS 14(3): 231-8.
7. Engel N, Oppermann C, Falodun A, Kragl U, (2011) Proliferative effects of five traditional Nigerian medicinal plant extracts on human breast and bone cancer cell lines. J Ethnopharmacol 137(2): 1003-10.
8. Abarzua S, Szewczyk M, Gailus S, Richter DU, Ruth W, et al. (2007) Effects of phytoestrogen extracts from Linum usitatissimum on the Jeg3 human trophoblast tumour cell line. Anticancer Res 27(4A): 2053-8.
9. Abarzua S, Drechsler S, Fischer K, Pietschmann N, Stapel J, et al. (2010) Online monitoring of cellular metabolism in the MCF-7 carcinoma cell line treated with phytoestrogen extracts. Anticancer Res 30(5): 1587-92.
10. Borgan E, Sitter B, Lingjærde OC, Johnsen H, Lundgren S, et al. (2010) Merging transcriptomics and metabolomics-advances in breast cancer profiling. BMC Cancer 10: 628.
11. Schramm G, Surmann EM, Wiesberg S, Oswald M, Reinelt G, et al. (2010) Analyzing the regulation of metabolic pathways in human breast cancer. BMC Med Genomics 3: 39.
12. Pyne NJ, Pyne S, (2010) Sphingosine 1-phosphate and cancer. Nat Rev Cancer 10(7): 489-503.
13. Pyne S, Bittman R, Pyne NJ, (2011) Sphingosine kinase inhibitors and cancer: seeking the golden sword of hercules. Cancer Res 71(21): 6576-82.
14. Pitson SM, (2010) Regulation of sphingosine kinase and sphingolipid signaling. Trends Biochem Sci 36(2): 97-107.
15. Long JS, Edwards J, Watson C, Tovey S, Mair KM, et al. (2010) Sphingosine kinase 1 induces tolerance to human epidermal growth factor receptor 2 and prevents formation of a migratory phenotype in response to sphingosine 1-phosphate in estrogen receptor-positive breast cancer cells. Mol Cell Biol 30(15): 3827-41.
16. Nebe B, Peters A, Duske K, Richter DU, Briese V, (2006) Influence of Phytoestrogens on the proliferation and expression of adhesion receptors in human and epithelial cells in vitro. Eur J Cancer Prev 15(5): 405-15.
17. Lisec J, Schauer N, Kopka J, Willmitzer L, Fernie AR, (2006) Gas chromatography mass spectrometry-based metabolite profiling in plants. Nat Protoc 1: 387-396.
18. Cuadros-Inostroza A, Caldana C, Redestig H, Kusano M, Lisec J, et al. (2009) TargetSearch-a Bioconductor package for the efficient preprocessing of GC-MS metabolite profiling data. BMC Bioinformatics 16 10: 428.
19. Lisec J, Römisch-Margl L, Nikoloski Z, Piepho H-P, Giavalisco P, et al. (2011) Corn hybrids display lower metabolite variability and complex metabolite inheritance patterns. Plant J 68(2): 326-336.
20. Bradford MM, (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-54.
21. Meyer TS, Lamberts BL, (1965) Use of Coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips. Biochim Biophys Acta 24 107(1): 144-5.
22. Thedinga E, Kob A, Holst H, Keuer A, Drechsler S, et al. (2007) Online monitoring of cell metabolism for studying pharmacodynamic effects. Toxicol Appl Pharmacol 220(1): 33-44.
23. Rebl H, Finke B, Schroeder K, Nebe JB, (2010) Time-dependent metabolic activity and adhesion of human osteoblast-like cells on sensor chips with a plasma polymer nanolayer. Int J Artif Organs 33(10): 738-48.
24. Stacklies W, Redestig H, Scholz M, Walther D, Selbig J, (2007) pcaMethods-a bioconductor package providing PCA methods for incomplete data. Bioinformatics 23(9): 1164-1167.
25. Wang C, Kurzer MS, (1997) Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells. Nutr Cancer 28(3): 236-47.
26. Wang TT, Sathyamoorthy N, Phang JM, (1996) Molecular effects of genistein on estrogen receptor mediated pathways. Carcinogenesis 17(2): 271-5.
27. de Lemos ML, (2001) Effects of soy phytoestrogens genistein and daidzein on breast cancer growth. Ann Pharmacother 35(9): 1118-21.
28. Low YL, Taylor JI, Grace PB, Dowsett M, Scollen S, Dunning AM, et al. (2005) Phytoestrogen exposure correlation with plasma estradiol in postmenopausal women in European Prospective Investigation of Cancer and Nutrition-Norfolk may involve diet-gene interactions. Cancer Epidemiol Biomarkers Prev 14(1): 213-20.
29. Kaelin WG Jr, Thompson CB, (2010) Q&A: Cancer: clues from cell metabolism. Nature 465(7298): 562-4.
30. Levine AJ, Puzio-Kuter AM, (2010) The control of the metabolic switch in cancers by oncogenes and tumor suppressor genes. Science 330(6009): 1340-4.
31. Ferreira AK, Meneguelo R, Neto SC, Chierice GO, Maria DA, (2011) Synthetic Phosphoethanolamine Induces Apoptosis Through Caspase-3 Pathway by Decreasing Expression of Bax/Bad Protein and Changes Cell Cycle in Melanoma. J Cancer Sci Ther 3: 053-059.
32. Tani M, Ito M, Igarashi Y, (2007) Ceramide/sphingosine/sphingosine 1-phosphate metabolism on the cell surface and in the extracellular space. Cell Signal 19(2): 229-37.