High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in escherichia coli

Engineering in Life Sciences

Volumn 12, Issue 5, 2012, Pages 574-582

  Yu, Yi ^a   Wei, Peilian ^b   Zhu, Xiangcheng ^c   Huang, Lei ^a   Cai, Jin ^a   Xu, Zhinan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Hunan Engineering Research Center of Combinatorial   (China)

Author keywords

Glucose dehydrogenase; Optimization; PQQGDH; Recombinant expression

Indexed keywords

ACINETOBACTER CALCOACETICUS; AFFINITY CHROMATOGRAPHY PURIFICATION; BIOPROCESS CONTROL; CARBON SOURCE; EXPRESSION LEVELS; FERMENTORS; GLUCOSE DEHYDROGENASE; GLUCOSE DETECTION; HIGH-LEVEL PRODUCTION; HIGH-PURITY; LARGE-SCALE PRODUCTION; NATIVE ENZYMES; OVER-EXPRESSION; PQQ-GDH; PRODUCTION PROCESS; PYRROLOQUINOLINE; RECOMBINANT ESCHERICHIA COLI; RECOMBINANT EXPRESSION; SPECIFIC ACTIVITY; SUBSTRATE SPECIFICITY; TARGET PROTEINS;

AFFINITY CHROMATOGRAPHY; ENZYME ACTIVITY; ESCHERICHIA COLI; OPTIMIZATION; PURIFICATION; SUBSTRATES;

GLUCOSE;

GLUCOSE DEHYDROGENASE; PYRROLOQUINOLINEQUINONE;

ANTIBIOTICS; COLIFORM BACTERIUM; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GLUCOSE; OPTIMIZATION; RECOMBINATION;

ACINETOBACTER CALCOACETICUS; ARTICLE; BIOPROCESS; BIOREACTOR; CARBON SOURCE; CONTROLLED STUDY; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN TARGETING; THERMOSTABILITY;

EID: 84867905286     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201100224     Document Type: Article

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