Stem cell recovering effect of copper-free GHK in skin

Journal of Peptide Science

Volumn 18, Issue 11, 2012, Pages 685-690

  Choi, Hye Ryung ^a   Kang, Youn A ^a   Ryoo, Sun Jong ^b   Shin, Jung Won ^a,b   Na, Jung Im ^a,b   Huh, Chang Hun ^a,b   Park, Kyoung Chan ^a,b  

^a Seoul National University Bundang Hospital   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

GHK; Skin equivalents; Stem cells

Indexed keywords

ALPHA6 INTEGRIN; BETA1 INTEGRIN; CHELATING AGENT; COPPER; GLYCINE HISTIDINE LYSINE; UNCLASSIFIED DRUG;

ARTICLE; BASEMENT MEMBRANE; CELL PROLIFERATION; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HUMAN TISSUE; KERATINOCYTE; MALE; MONOLAYER CULTURE; NORMAL HUMAN; PRIORITY JOURNAL; STEM CELL; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; COPPER; HUMANS; IMMUNOHISTOCHEMISTRY; INTEGRINS; KERATINOCYTES; MODELS, BIOLOGICAL; OLIGOPEPTIDES; SKIN; STEM CELLS;

EID: 84867900898     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.2455     Document Type: Article

References (18)

1. Witt KA, Gillespie TJ, Huber JD, Egleton RD, Davis TP. Peptide drug modifications to enhance bioavailability and blood-brain barrier permeability. Peptides 2001; 22: 2329-2343.
2. Zhang L, Falla TJ. Cosmeceuticals and peptides. Clin. Dermatol. 2009; 27: 485-494.
3. Trapaidze A, Hureau C, Bal W, Winterhalter M, Faller P. Thermodynamic study of cu2+ binding to the DAHK and GHK peptides by isothermal titration calorimetry (ITC) with the weaker competitor glycine. J. Biol. Inorg. Chem.: JBIC: a publication of the Society of Biological Inorganic Chemistry 2012; 17: 37-47.
4. Pollard JD, Quan S, Kang T, Koch RJ. Effects of copper tripeptide on the growth and expression of growth factors by normal and irradiated fibroblasts. Arch. Facial Plast. Surg.: official publication for the American Academy of Facial Plastic and Reconstructive Surgery, Inc and the International Federation of Facial Plastic Surgery Societies 2005; 7: 27-31.
5. Wegrowski Y, Maquart FX, Borel JP. Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex glycyl- l-histidyl- l-lysine-cu2+. Life Sci. 1992; 51: 1049-1056.
6. Maquart FX, Pickart L, Laurent M, Gillery P, Monboisse JC, Borel JP. Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex glycyl- l-histidyl- l-lysine-cu2+. FEBS Lett. 1988; 238: 343-346.
7. Kang YA, Choi HR, Na JI, Huh CH, Kim MJ, Youn SW, Kim KH, Park KC. Copper-GHK increases integrin expression and p63 positivity by keratinocytes. Arch. Dermatol. Res. 2009; 301: 301-306.
8. Tenaud I, Sainte-Marie I, Jumbou O, Litoux P, Dreno B. In vitro modulation of keratinocyte wound healing integrins by zinc, copper and manganese. Br. J. Dermatol. 1999; 140: 26-34.
9. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975; 6: 331-343.
10. Kim DS, Cho HJ, Choi HR, Kwon SB, Park KC. Isolation of human epidermal stem cells by adherence and the reconstruction of skin equivalents. Cell. Mol. Life Sci.: CMLS 2004; 61: 2774-2781.
11. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Natl. Acad. Sci. U.S.A. 1979; 76: 1274-1278.
12. Sonnenberg A, Calafat J, Janssen H, Daams H, van der Raaij-Helmer LM, Falcioni R, Kennel SJ, Aplin JD, Baker J, Loizidou M, Garrod D. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion. J. Cell Biol. 1991; 113: 907-917.
13. Grose R, Hutter C, Bloch W, Thorey I, Watt FM, Fassler R, Brakebusch C, Werner S. A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair. Development (Cambridge, England) 2002; 129: 2303-2315.
14. Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, Bondanza S, Ponzin D, McKeon F, De Luca M. P63 identifies keratinocyte stem cells. Proc. Natl. Acad. Sci. U.S.A. 2001; 98: 3156-3161.
15. Bravo R, Frank R, Blundell PA, Macdonald-Bravo H. Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature 1987; 326: 515-517.
16. Ivanova IA, D'Souza SJ, Dagnino L. Signalling in the epidermis: the E2f cell cycle regulatory pathway in epidermal morphogenesis, regeneration and transformation. Int. J. Biol. Sci. 2005; 1: 87-95.
17. Wha Kim S, Lee IW, Cho HJ, Cho KH, Han Kim K, Chung JH, Song PI, Chan Park K. Fibroblasts and ascorbate regulate epidermalization in reconstructed human epidermis. J. Dermatol. Sci. 2002; 30: 215-223.
18. Kim SY, Cho HJ, Kim DS, Choi HR, Kwon SB, Na JI, Jeon HC, Huh CH, Youn SW, Cho KH, Park KC. Differential expression of p63 isoforms in normal skin and hyperproliferative conditions. J. Cutan. Pathol. 2009; 36: 825-830.