Expression, purification and analysis of an Arabidopsis recombinant CBL-interacting protein kinase3 (CIPK3) and its constitutively active form

Protein Expression and Purification

Volumn 86, Issue 1, 2012, Pages 45-52

  Gao, Peng ^a   Kolenovsky, Allan ^a   Cui, Yuhai ^b   Cutler, Adrian J ^a   Tsang, Edward W T ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

Affinity purification; CIPK3; Enzymatically active; Inclusion bodies; Peptide phosphorylation; Protein kinase; Sarkosyl; Site directed mutagenesis

Indexed keywords

ARABIDOPSIS PROTEIN; ASPARTIC ACID; CIPK3 PROTEIN, ARABIDOPSIS; DRUG DERIVATIVE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PROTEIN SERINE THREONINE KINASE; SARCOSINE; SARKOSYL; THREONINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; CELL INCLUSION; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; SOLUBILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARABIDOPSIS PROTEINS; ASPARTIC ACID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; SARCOSINE; SEQUENCE ALIGNMENT; SOLUBILITY; THREONINE;

EID: 84867875116     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.08.013     Document Type: Article

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