Direct estimation of sialic acid in milk and milk products by fluorimetry and its application in detection of sweet whey adulteration in milk

Journal of Dairy Research

Volumn 79, Issue 4, 2012, Pages 495-501

  Neelima, ^a   Rao, Priyanka Singh ^a   Sharma, Rajan ^a   Rajput, Yudhishthir S ^b  

^a NATIONAL DAIRY RESEARCH INSTITUTE   (India)

^b Division of Animal Biochemistry   (India)

Author keywords

adulteration; Fluorimetry; Glycomacropeptide; Milk; Sialic acid; Sweet whey

Indexed keywords

CASEIN; CASEINOMACROPEPTIDE; MILK PROTEIN; N ACETYLNEURAMINIC ACID; PEPTIDE FRAGMENT;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; DAIRY PRODUCT; FEMALE; FOOD CONTAMINATION; METABOLISM; MILK; PRECIPITATION; SPECTROFLUOROMETRY;

ANIMALS; CASEINS; CATTLE; CHEMICAL PRECIPITATION; DAIRY PRODUCTS; FEMALE; FOOD CONTAMINATION; MILK; MILK PROTEINS; N-ACETYLNEURAMINIC ACID; PEPTIDE FRAGMENTS; SPECTROMETRY, FLUORESCENCE;

EID: 84867798511     PISSN: 00220299     EISSN: 14697629     Source Type: Journal    
DOI: 10.1017/S0022029912000441     Document Type: Article

References (30)

1. Aminoff D 1961 Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochemical Journal 81 384-392.
2. Bremer MGEG, Kemmers-Voncken AEM, Boers EAM, Frankhuizen R & Haasnoot W 2008 Enzyme-linked immunosorbent assay for the detection of rennet whey powder in milk and buttermilk powder. International Dairy Journal 18 294-302. (Pubitemid 350166916)
3. Chavez NA, Salinas E, Jauregui J, Palomares LA & Macias K 2008 Detection of bovine milk adulterated with cheese whey by western blot immunoassay. Food and Agricultural Immunology 19 265-272.
4. Cherkaoui S, Doumenc N, Tachon P, Neeser JR & Veuthey JL 1997 Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination. Journal of Chromatography A 790 195-205. (Pubitemid 28020403)
5. de Carvalho BMA, de Carvalho LM, Alcântra LAP & Bonomo RCF 2007 Métodos de detecção de fraude em leite por adição de soro de queijo. REDVET. Revista electrónica de Veterinaria 8 1-7.
6. Fernandez A, Menendez V, Riera FA & Alvarez R 2011 Caseinomacropeptide behaviour in a whey protein fractionation process based on α-lactalbumin precipitation. Journal of Dairy Research 78 196-202.
7. Fernando SF & Woonton BW 2010 Quantitation of N-acetylneuraminic (sialic) acid in bovine glycomacropeptide (GMP). Journal of Food Composition and Analysis 23 359-366.
8. Josephson R, Holloway-Thomas DJ & Warthesen J 1980 Cheese whey detection in frozen desserts. Journal of Dairy Science 63 1356-1360.
9. Koliwer-Brandl H, Siegert N, Umnus K, Kelm A, Tolkach A, Kulozik U, Kuballa J, Cartellieri S & Kelm S 2011 Lectin inhibition assays for the analysis of bioactive milk sialoglycoconjugates. International Dairy Journal 21 413-420.
10. Koning P, Elisses J & De Vries H 1966 A method for the detection of small percentages of whey powder in milk powder. Netherlands Milk and Dairy Journal 20 203-212.
11. Lacomba R, Salcedo J, Alegria A, Barbera R, Hueso P, Matencio E & Lagarda MJ 2011 Sialic acid (N-acetyl and N-glycolylneuraminic acid) and ganglioside in whey protein concentrates and infant formulae. International Dairy Journal 21 887-895.
12. Lacomba R, Salcedo J, Alegria A, Lagarda MJ, Barbera R & Matencio E 2010 Determination of sialic acid and gangliosides in biological samples and dairy products: A review. Journal of Pharmaceutical and Biomedical Analysis 51 346-357.
13. Lynch J 2005 Appendix E: laboratory quality assurance In Official Method of Analysis of AOAC International, (Eds W Horwitz & GW JrLatimer). Geneva, Switzerland: AOAC International.
14. Martin MJ, Vázquez E & Rueda R 2007 Application of a sensitive fluorometric HPLC assay to determine the sialic acid content of infant formulas. Analytical and Bioanalytical Chemistry 387 2943-2949. (Pubitemid 46479960)
15. Martin-Hernandez C, Munoz M, Daury C, Weymuth H, Kemmers-Voncken AEM, Corbation V, Toribio T & Bremer MGEG 2009 Immunochromatographic lateral-flow test strip for the rapid detection of added bovine rennet whey in milk and milk powder. International Dairy Journal 19 205-208.
16. Matsuno K & Suzuki S 2008 Simple fluorimetric method for quantification of sialic acids in glycoproteins. Analytical Biochemistry 375 53-59.
17. Medndham J, Denney RC, Barnes JD & Thomas MJK 2000 Vogels's Textbook of Quantitative Chemical Analysis. 6th edition. New Delhi, India: Pearson Education (Singapore) Pte. Ltd.
18. Nakano T, Ikawa N&, Ozimek L 2007 Detection of sialylated phosphorylated k-casein glycomacropeptide electrophoresed on polyacrylamide gels and cellulose acetate strips by the thiobarbituric acid and malachite green dye reactions. Journal of Agricultural and Food Chemistry 55 2714-2726. (Pubitemid 46623770)
19. Nakano T & Ozimek L 1999 Determination of sialic acid by the thiobarbituric acid reaction in sweet whey and its fractions. Journal of Agriculture and Food Chemistry 47 2613-2616. (Pubitemid 29396328)
20. Romero EL, Pardo MF, Porro S & Alonso S 1997 Sialic acid measurement by a modified Aminoff method: a time-saving reduction in 2-thiobarbituric acid concentration. Journal of Biochemical and Biophysical Methods 35 129-134. (Pubitemid 27414543)
21. Saito T, Yamaji A & Itoh T 1991 A new isolation method of caseinoglycopeptide from sweet cheese whey. Journal of Dairy Science 74 2831-2837.
22. Salcedo J, Lacomba R, Alegria A, Barbera R, Matencio E & Lagarda MJ 2011 Comparison of spectrophotometric and HPLC methods for determining sialic acid in infant formulas. Food Chemistry 127 1905-1910.
23. Spichtig V, Michaud J & Austin S 2010 Determination of sialic acids in milk and milk based products. Analytical Biochemistry 405 28-40.
24. Sugahara K, Sugimoto K, Nomura O & Usui T 1980 Enzymatic assay of serum sialic acid. Clinica Chimica Acta 108 493-498. (Pubitemid 11192723)
25. Teshima S, Tamal K, Hayashi Y & Emi S 1988 New enzymatic determination of sialic acid in serum. Clinical Chemistry 34 2291-2294. (Pubitemid 19001256)
26. Thoma C, Krause I & Kulozik U 2006 Precipitation behaviour of caseinomacropeptide and their simultaneous determination with whey proteins by RP-HPLC. International Dairy Journal 16 285-293. (Pubitemid 43061177)
27. Thoma-Worringer C, Sorensen J & Lopez-Fandino R 2006 Health effects and technological features of caseinomacropeptide. International Dairy Journal 16 1324-1333. (Pubitemid 44348878)
28. Wang B & Brand-Miller J 2003 The role and potential of sialic acid in human nutrition. European Journal of Clinical Nutrition 57 1351-1369. (Pubitemid 37407130)
29. Wang B, Brand-Miller J, McVeagh P & Petocz P 2001 Concentration and distribution of sialic acid in human milk and infant formulas. American Journal of Clinical Nutrition 74 510-515. (Pubitemid 32880262)
30. Warren L 1959 The thiobarbituric acid assay of sialic acids. Journal of Biological Chemistry 234 1971-1974.