Quantitative and specific detection of the biocontrol agent, Serratia plymuthica, in plant extracts using a real-time TaqMan® assay

Journal of Applied Genetics

Volumn 53, Issue 4, 2012, Pages 457-467

  Czajkowski, Robert ^a,b   van der Wolf, Jan M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NETHERLANDS INSTITUTE OF ECOLOGY NIOO KNAW   (Netherlands)

Author keywords

Antagonism; Biological control; Potato; Selective medium; Tomato

Indexed keywords

BACTERIAL DNA; PLANT EXTRACT; SOLANUM TUBEROSUM EXTRACT; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; BIOLOGICAL CONTROL AGENT; CONTROLLED STUDY; DRUG DETERMINATION; ECOSYSTEM; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLANT GROWTH; PLANT ROOT; PLANT SEED; POTATO; POTATO SPINDLE TUBER; REAL TIME POLYMERASE CHAIN REACTION; SERRATIA PLYMUTHICA;

BACTERIAL LOAD; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL CONTROL AGENTS; CARBON-SULFUR LYASES; CONSENSUS SEQUENCE; CULTURE MEDIA; DNA, BACTERIAL; GENES, BACTERIAL; LYCOPERSICON ESCULENTUM; PHYLOGENY; PLANT DISEASES; PLANT EXTRACTS; PLANT ROOTS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SERRATIA; SOIL MICROBIOLOGY; SOLANUM TUBEROSUM; TIME FACTORS;

EID: 84867766980     PISSN: 12341983     EISSN: None     Source Type: Journal    
DOI: 10.1007/s13353-012-0106-0     Document Type: Article

References (51)

1. Alström S, Gerhardson B (1987) Characteristics of a Serratia plymuthica isolate from plant rhizospheres. Plant Soil 103(2): 185-189.
2. Benhamou N, Gagné S, Le Quéré D, Dehbi L (2000) Bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathology 90(1): 45-56.
3. Berg G (2000) Diversity of antifungal and plant-associated Serratia plymuthica strains. J Appl Microbiol 88(6): 952-960.
4. Berg G, Opelt K, Zachow C, Lottmann J, Götz M, Costa R, Smalla K (2006) The rhizosphere effect on bacteria antagonistic towards the pathogenic fungus Verticillium differs depending on plant species and site. FEMS Microbiol Ecol 56(2): 250-261.
5. Cao JG, Meighen EA (1989) Purification and structural identification of an autoinducer for the luminescence system of Vibrio harveyi. J Biol Chem 264(36): 21670-21676.
6. Czajkowski R, de Boer WJ, van Veen JA, van der Wolf JM (2012a) Characterization of bacterial isolates from rotting potato tuber tissue showing antagonism to Dickeya sp. biovar 3 in vitro and in planta. Plant Pathol 61(1): 169-182.
7. Czajkowski R, de Boer WJ, van Veen JA, van der Wolf JM (2012b) Studies on the interaction between the biocontrol agent, Serratia plymuthica A30, and blackleg-causing Dickeya sp. (biovar 3) in potato (Solanum tuberosum). Plant Pathol (in press). doi: 10. 1111/j. 1365-3059. 2011. 02565. x.
8. Dandurishvili N, Toklikishvili N, Ovadis M, Eliashvili P, Giorgobiani N, Keshelava R, Tediashvili M, Vainstein A, Khmel I, Szegedi E, Chernin L (2011) Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants. J Appl Microbiol 110(1): 341-352.
9. De Vleesschauwer D, Höfte M (2007) Using Serratia plymuthica to control fungal pathogens of plants. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 2, no. 46.
10. De Vleesschauwer D, Chernin L, Höfte MM (2009) Differential effectiveness of Serratia plymuthica IC1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice. BMC Plant Biol 9: 9.
11. Felsenstein J (1993) {PHYLIP}: Phylogenetic inference package, version 3. 5c, Department of Genetics, University of Washington, Seattle.
12. Fujimura M, Kato J, Tosa T, Chibata I (1984) Continuous production of L-arginine using immobilized growing Serratia marcescens cells: effectiveness of supply of oxygen gas. Appl Microbiol Biotech 19(2): 79-84.
13. Garbeva P, Voesenek K, van Elsas JD (2004) Quantitative detection and diversity of the pyrrolnitrin biosynthetic locus in soil under different treatments. Soil Biol Biochem 36(9): 1453-1463.
14. Gavini F, Ferragut C, Izard D, Trinel PA, Leclerc H, Lefebvre B, Mossel DAA (1979) Serratia fonticola, a new species from water. Int J Syst Bacteriol 29(2): 92-101.
15. Grimont PAD, Grimont F (1978) The genus Serratia. Annu Rev Microbiol 32(1): 221-248.
16. Grimont PAD, Grimont F (1981) The genus Serratia. Springer, Berlin, Heidelberg, New York.
17. Grimont PAD, Grimont F (1984) Genus VIII. Serratia Bizio 1823, 288AL. Williams and Wilkins, Baltimore.
18. Grimont PAD, Grimont F, Dulong de Rosnay HLC, Sneath PHA (1977) Taxonomy of the genus Serratia. J Gen Microbiol 98(1): 39-66.
19. Grimont PAD, Grimont F, Richard C, Davis BR, Steigerwalt AG, Brenner DJ (1978) Deoxyribonucleic acid relatedness between Serratia plymuthica and other Serratia species, with a description of Serratia odorifera sp. nov. (type strain: ICPB 3995). Int J Syst Bacteriol 28(4): 453-463.
20. Grimont PAD, Grimont F, Lysenko O (1979) Species and biotype identification of Serratia strains associated with insects. Curr Microbiol 2(3): 139-142.
21. Grimont PAD, Jackson TA, Ageron E, Noonan MJ (1988) Serratia entomophila sp. nov. associated with amber disease in the New Zealand grass grub Costelytra zealandica. Int J Syst Bacteriol 38(1): 1-6.
22. Harms G, Layton AC, Dionisi HM, Gregory IR, Garrett VM, Hawkins SA, Robinson KG, Sayler GS (2002) Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant. Environ Sci Technol 37(2): 343-351.
23. Inceoǧlu O, Salles JF, van Overbeek L, van Elsas JD (2010) Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields. Appl Environ Microbiol 76(11): 3675-3684.
24. Jafra S, Przysowa J, Czajkowski R, Michta A, Garbeva P, van der Wolf JM (2006) Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone. Can J Microbiol 52(10): 1006-1015.
25. Jafra S, Przysowa J, Gwizdek-Wiśniewska A, van der Wolf JM (2009) Potential of bulb-associated bacteria for biocontrol of hyacinth soft rot caused by Dickeya zeae. J Appl Microbiol 106: 268-277.
26. Johnson VW, Pearson JF, Jackson TA (2001) Formulation of Serratia entomophila for biological control of grass grub. NZ Plant Protect 54: 125-127.
27. Kamensky M, Ovadis M, Chet I, Chernin L (2003) Soil-borne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases. Soil Biol Biochem 35(2): 323-331.
28. Kobayashi DY, Guglielmoni M, Clarke BB (1995) Isolation of the chitinolytic bacteria Xanthomonas maltophilia and Serratia marcescens as biological control agents for summer patch disease of turfgrass. Soil Biol Biochem 27(11): 1479-1487.
29. Krastanov A, Blazheva D, Yanakieva I, Kratchanova M (2006) Conversion of sucrose into palatinose in a batch and continuous processes by immobilized Serratia plymuthica cells. Enzyme Microb Technol 39(6): 1306-1312.
30. Kurze S, Bahl H, Dahl R, Berg G (2001) Biological control of fungal strawberry diseases by Serratia plymuthica HRO-C48. Plant Dis 85(5): 529-534.
31. Lerat E, Moran NA (2004) The evolutionary history of quorum-sensing systems in bacteria. Mol Biol Evol 21(5): 903-913.
32. Levenfors JJ, Hedman R, Thaning C, Gerhardson B, Welch CJ (2004) Broad-spectrum antifungal metabolites produced by the soil bacterium Serratia plymuthica A 153. Soil Biol Biochem 36(4): 677-685.
33. Lovell DJ, Bibel DJ (1977) Tween 80 medium for differentiating nonpigmented Serratia from other Enterobacteriaceae. J Clin Microbiol 5: 245-247.
34. Meziane H, Gavriel S, Ismailov Z, Chet I, Chernin L, Höfte M (2006) Control of green and blue mould on orange fruit by Serratia plymuthica strains IC14 and IC1270 and putative modes of action. Postharvest Biol Technol 39(2): 125-133.
35. Pang Y, Liu X, Ma Y, Chernin L, Berg G, Gao K (2009) Induction of systemic resistance, root colonisation and biocontrol activities of the rhizospheric strain of Serratia plymuthica are dependent on N-acyl homoserine lactones. Eur J Plant Pathol 124(2): 261-268.
36. Postma J, Schilder MT, Bloem J, van Leeuwen-Haagsma WK (2008) Soil suppressiveness and functional diversity of the soil microflora in organic farming systems. Soil Biol Biochem 40(9): 2394-2406.
37. Ramirez MJ (1968) Differentiation of Klebsiella-Enterobacter (Aerobacter)- Serratia by biochemical tests and antibiotic susceptibility. Appl Microbiol 16: 1548-1550.
38. Rodgers JL, Nicewander WA (1988) Thirteen ways to look at the correlation coefficient. Am Stat 42(1): 59-66.
39. Scherwinski K, Wolf A, Berg G (2007) Assessing the risk of biological control agents on the indigenous microbial communities: Serratia plymuthica HRO-C48 and Streptomyces sp. HRO-71 as model bacteria. BioControl 52(1): 87-112.
40. Shen S-S, Choi O-H, Lee S-M, Park C-H (2002) In vitro and in vivo activities of a biocontrol agent, Serratia plymuthica A21-4, against Phytophthora capsici. The Plant Pathol J 18(4): 221-224.
41. Sneh B, Agami O, Baker R (1985) Biological control of Fusarium-wilt in carnation with Serratia liquefaciens and Hafnia alvei isolated from rhizosphere of carnation. J Phytopathol 113(3): 271-276.
42. Someya N, Kataoka N, Komagata T, Hirayae K, Hibi T, Akutsu K (2000) Biological control of cyclamen soilborne diseases by Serratia marcescens strain B2. Plant Dis 84(3): 334-340.
43. Sun J, Daniel R, Wagner-Döbler I, Zeng A-P (2004) Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways. BMC Evol Biol 4(1): 36.
44. Trifonova R, Postma J, Verstappen FWA, Bouwmeester HJ, Ketelaars JJMH, van Elsas J-D (2008) Removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms. FEMS Microbiol Ecol 66(1): 158-166.
45. van der Wolf JM, van Beckhoven JRCM, de Boef E, Roozen NJM (1993) Serological characterization of fluorescent Pseudomonas strains cross-reacting with antibodies against Erwinia chrysanthemi. Eur J Plant Pathol 99(2): 51-60.
46. Vivas J, González JA, Barbeyto L, Rodríguez LA (2000) Identification of environmental Serratia plymuthica strains with the new combo panels type 1S. Mem Inst Oswaldo Cruz 95: 227-229.
47. Wada M, Uchida T, Kato J, Chibata I (1980) Continuous production of L-isoleucine using immobilized growing Serratia marcescens cells. Biotechnol Bioeng 22(6): 1175-1188.
48. Winzer K, Hardie KR, Williams P (2003) LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. Adv Appl Microbiol 53: 291-396.
49. Wolf PL, Von der Muehll E, Praisler K (1973) A test for bacterial alkaline phosphatase: use in rapid identification of Serratia organisms. Clin Chem 19(11): 1248-1249.
50. Yang S, Lin S, Kelen GD, Quinn TC, Dick JD, Gaydos CA, Rothman RE (2002) Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens. J Clin Microbiol 40(9): 3449-3454.
51. Zabransky RJ, Hall JW, Day FE, Needham GM (1969) Klebsiella, Enterobacter, and Serratia: biochemical differentiation and susceptibility to ampicillin and three cephalosporin derivatives. Appl Microbiol 18(2): 198-203.