A novel scavenger receptor 5-based antibiotic-independent selection method for generation of stable recombinant protein-producing mammalian cell lines especially suitable for proteins affecting cell adhesion

BioTechniques

Volumn 53, Issue 4, 2012, Pages 221-230

  Ojala, Juha Risto Matias ^a   Pikkarainen, Timo ^a   Domogatskaya, Anna ^a   Tryggvason, Karl ^a   Rodin, Sergey ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Improvement of adhesion; Mammalian cell lines; Recombinant proteins; Stable transfection

Indexed keywords

ANTIBIOTIC AGENT; MEMBRANE PROTEIN; MESSENGER RNA; PROTEIN SCAVENGER RECEPTOR OF THE CLASS A; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL ASSAY; CELL CLONE; CELL LINE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; PROTEIN EXPRESSION; STABLE RECOMBINANT PROTEIN PRODUCING MAMMALIAN CELL LINE;

ANIMALS; BLOTTING, WESTERN; CELL ADHESION; CELL CULTURE TECHNIQUES; DENSITOMETRY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC VECTORS; HEK293 CELLS; HUMANS; LAMININ; MICE; RECOMBINANT PROTEINS; SCAVENGER RECEPTORS, CLASS A;

EID: 84867760219     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/0000113936     Document Type: Article

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