Human pIgR mimetic peptidic ligand for affinity purification of IgM Part II: Ligand binding characteristics

Separation and Purification Technology

Volumn 102, Issue , 2013, Pages 43-49

  Gautam, Satyen ^a   Loh, Kai Chee ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Affinity chromatography; Biomimetic peptidic ligand; IgM; pIgR

Indexed keywords

ADVERSE EFFECT; AFFINITY LIGANDS; AFFINITY PURIFICATION; ASSOCIATION CONSTANT; BINDING CAPACITIES; BOVINE SERUM ALBUMINS; EQUILIBRIUM STUDY; IGM; IMMUNOGLOBULIN RECEPTORS; LIGAND BINDING; PEPTIDIC LIGANDS; PIGR; SODIUM HYDROXIDES; SYNTHETIC MIXTURES;

AFFINITY CHROMATOGRAPHY; BIOMIMETICS; ETHANOL; IONIC STRENGTH; MAMMALS; MICROSPHERES; PURIFICATION; SILICA; SODIUM; SODIUM CHLORIDE;

LIGANDS;

BOVINAE; ORYCTOLAGUS CUNICULUS;

EID: 84867743008     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2012.09.024     Document Type: Article

References (10)

1. V.P. Knutson, R.A. Buck, and R.M. Moreno Purification of a murine monoclonal antibody of the IgM class J. Immunol. Method 136 1991 151 157
2. D. Roggenbuck, U. Marx, S.T. Kiessig, G. Schoenherr, S. Jahn, and T. Porstmann Purification and immunochemical characterization of a natural human polyreactive monoclonal IgM antibody J. Immunol. Method 167 1994 207 218
3. P. Gagnon, F. Hensel, and R. Richieri Purification of IgM monoclonal antibodies Biopharm Int. Suppl. 2008 26 36
4. C.L. Jones, G.M. Georgiou, K.J. Fowler, P.I. Wajngarten, and D.M. Roberton Purification of polymeric immunoglobulin from cell culture supernatants by affinity chromatography using secretory component J. Immunol. Method 104 1987 237 243
5. 2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin Arch. Biochem. Biophys. 267 1988 676 680
6. A. Nethery, R.L. Raison, and S.B.E. Smith Single-step purification of immunoglobulin M on Clq-Sepharose J. Immunol. Method 126 1990 57 60
7. J.R. Nevens, A.K. Mallia, M.W. Wendt, and P.K. Smith Affinity chromatographic purification of immunoglobulin M antibodies utilizing immobilized mannan binding protein J. Chrom. 597 1992 247 256
8. S. Gautam, and K.C. Loh Human pIgR mimetic peptidic ligand for affinity purification of IgM Separ. Purif. Technol 102 2012 173 179
9. C. Prin, M.C. Bene, B. Gobert, P. Montagne, and G.C. Faure Isoelectric restriction of human immunoglobulin isotypes Biochim. Biophys. Acta 1243 1995 287 289
10. K.E. Bernstein, C.B. Alexander, E.P. Reddy, and R.G. Mage Complete sequence of a cloned cDNA encoding rabbit secreted mu-chain of VHa2 allotype: comparisons with VHa1 and membrane mu sequences J. Immunol. 132 1984 490 495