Development and validation of automated SPE-HPLC-MS/MS methods for the quantification of asenapine, a new antipsychotic agent, and its two major metabolites in human urine

Biomedical Chromatography

Volumn 26, Issue 12, 2012, Pages 1461-1463

  de Boer, Theo ^a   Meulman, Erik ^a   Meijering, Henri ^a   Wieling, Jaap ^a   Dogterom, Peter ^b   Lass, Holger ^c  

^a QPS Netherlands   (Netherlands)

^b NV ORGANON   (Netherlands)

^c Nuvisan Analytical Services GmbH   (Germany)

Author keywords

Asenapine; Automated; Liquid chromatography tandem mass spectrometry; Quantification; Solid phase extraction; Urine

Indexed keywords

BODY FLUIDS; DRUG PRODUCTS; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHASE SEPARATION;

ASENAPINE; AUTOMATED; GLUCURONIDES; HUMAN URINE; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY; QUANTIFICATION; SOLID-PHASE EXTRACTION; SPE-HPLC-MS; URINE;

METABOLITES;

ASENAPINE; ASENAPINE N GLUCURONIDE; DRUG METABOLITE; N DESMETHYLASENAPINE; UNCLASSIFIED DRUG;

ARTICLE; DRUG METABOLISM; DRUG URINE LEVEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; SOLID PHASE EXTRACTION HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY;

EID: 84867714108     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.2722     Document Type: Article

References (4)

1. De Boer T, Meulman E, Meijering H, Wieling J, Dogterom P and Lass H. Quantification of asenapine and three metabolites in human plasma using liquid chromatography-tandem mass spectrometry with automated solid-phase extraction: application to a phase I clinical trial with asenapine in healthy Caucasian subjects. Biomedical Chromatography 2012; 26(2): 156-165.
2. Food and Drug Administration, US Department of Health and Human Services, Center for Drug Evaluation and Research and Center for Veterinary Medicine. Guidance for Industry: Bioanalytical Method Validation, May 2001. Available from: http://www.fda.gov/CDER/GUIDANCE/4252fnl.htm
3. Shadid M, Walker GB, Zorn SH and Wong EH. Asenapine: a novel psychopharmacologic agent with a unique human receptor signature. Journal of Psychopharmacology 2009; 23(1): 65-73.
4. Van de Wetering-Krebbers SFM, Jacobs PL, Kemperman GJ, Spaans E, Peeters PAM, Delbressine LPC and Van Iersel MLPS. Metabolism and excretion of asenapine in healthy male subjects. Drug Metabolism and Disposition 2011; 39(4): 580-590.