Structure of AMP-PNP-bound vitamin B 12 transporter BtuCD-F

Nature

Volumn 490, Issue 7420, 2012, Pages 367-372

  Korkhov, Vladimir M ^a   Mireku, Samantha A ^a   Locher, Kaspar P ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; ADENYLYLIMIDODIPHOSPHATE; CYANOCOBALAMIN; DIMER; LIPOSOME; TRANSPORTER BINDING PROTEIN COMPLEX BTUCD BTUF; UNCLASSIFIED DRUG;

BIOLOGICAL UPTAKE; COLIFORM BACTERIUM; CYTOPLASM; MEMBRANE; SULFIDE; VITAMIN;

ARTICLE; CONTROLLED STUDY; CROSS LINKING; CRYSTAL STRUCTURE; CYTOPLASM; DISULFIDE BOND; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN STRUCTURE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ADENYLYL IMIDODIPHOSPHATE; AMINO ACID SEQUENCE; ATP-BINDING CASSETTE TRANSPORTERS; CRYSTALLOGRAPHY, X-RAY; CYTOPLASM; DISULFIDES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MAGNESIUM; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTANT PROTEINS; MUTATION; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SUBUNITS; STRUCTURE-ACTIVITY RELATIONSHIP; VITAMIN B 12;

ESCHERICHIA COLI;

EID: 84867670628     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature11442     Document Type: Article

References (44)

1. Holland, I. B., Cole, S. P. C., Kuchler, K. & Higgins, C. F. ABC Proteins: From Bacteria to Man (Academic, 2003).
2. Davidson, A. L., Dassa, E., Orelle, C. & Chen, J. Structure, function, and evolution of bacterial ATP-binding cassette systems. Microbiol. Mol. Biol. Rev. 72, 317-364 (2008).
3. Rees, D. C., Johnson, E. & Lewinson, O. ABC transporters: the powertochange. Nat. Rev. Mol. Cell Biol. 10, 218-227 (2009).
4. Hollenstein, K., Dawson, R. J. & Locher, K. P. Structure and mechanism of ABC transporter proteins. Curr. Opin. Struct. Biol. 17, 412-418 (2007).
5. Erkens, G. B. et al. The structural basis of modularity inECF-type ABC transporters. Nature Struct. Mol. Biol. 18, 755-760 (2011).
6. Reynolds, P. R., Mottur, G. P. & Bradbeer, C. Transportofvitamin B12 inEscherichia coli. Some observationsonthe rolesofthe gene productsofBtuC and TonB. J. Biol. Chem. 255, 4313-4319 (1980).
7. DeVeaux, L. C. & Kadner, R. J. Transport of vitamin B12 in Escherichia coli: cloning of the btuCD region. J. Bacteriol. 162, 888-896 (1985).
8. Pattery, T., Hernalsteens, J. P. & De Greve, H. Identification and molecular characterization of a novel Salmonella enteritidis pathogenicity islet encoding an ABC transporter. Mol. Microbiol. 33, 791-805 (1999).
9. Janakiraman, A. & Slauch, J. M. The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium. Mol. Microbiol. 35, 1146-1155 (2000).
10. Locher, K. P., Lee, A. T. & Rees, D. C. The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism. Science 296, 1091-1098 (2002).
11. Hvorup, R. N. et al. Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF. Science 317, 1387-1390 (2007)
12. Korkhov, V. M., Mireku, S. A., Hvorup, R. N. & Locher, K. P. Asymmetric states of vitamin B12 transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF. FEBS Lett. 586, 972-976 (2012).
13. Dawson, R. J. & Locher, K. P. Structure of a bacterial multidrug ABC transporter. Nature 443, 180-185 (2006).
14. Ward, A., Reyes, C. L., Yu, J., Roth, C. B. & Chang, G. Flexibility inthe ABC transporter MsbA:alternating accesswithatwist. Proc. Natl Acad. Sci. USA104, 19005-19010 (2007).
15. Oldham, M. L., Khare, D., Quiocho, F. A., Davidson, A. L. & Chen, J. Crystal structure of a catalytic intermediate of the maltose transporter. Nature 450, 515-521 (2007).
16. Oldham, M. L. & Chen, J. Crystal structure of the maltose transporter in a pretranslocation intermediate state. Science 332, 1202-1205 (2011).
17. 12 uptake. Biochemistry 44, 16301-16309 (2005).
18. Lewinson, O., Lee, A. T., Locher, K. P. & Rees, D. C. Adistinct mechanism for the ABC transporter BtuCD-BtuF revealed by the dynamics of complex formation. Nature Struct. Mol. Biol. 17, 332-338 (2010).
19. Klein, J. S. & Lewinson, O. Bacterial ATP-driven transporters of transition metals: physiological roles, mechanisms of action, and roles in bacterial virulence. Metallomics 3, 1098-1108 (2011).
20. Pinkett, H. W., Lee, A. T., Lum, P., Locher, K. P. & Rees, D. C. An inward-facing conformation of a putative metal-chelate-type ABC transporter. Science 315, 373-377 (2007).
21. Smith, P. C. et al. ATP bindingtothe motor domain fromanABC transporter drives formation of a nucleotide sandwich dimer. Mol. Cell 10, 139-149 (2002).
22. Zaitseva, J., Jenewein, S., Jumpertz, T., Holland, I. B. & Schmitt, L. H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB. EMBO J. 24, 1901-1910 (2005).
23. Procko, E., Ferrin-O'Connell, I., Ng, S. L. & Gaudet, R. Distinct structural and functional properties of the ATPase sites in an asymmetric ABC transporter. Mol. Cell 24, 51-62 (2006).
24. Dawson, R. J. & Locher, K. P. Structureof the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP. FEBS Lett. 581, 935-938 (2007).
25. Newstead, S. et al. Insights into how nucleotide-binding domains power ABC transport. Structure 17, 1213-1222 (2009).
26. Hopfner, K. P. et al. Structural biology of Rad50 ATPase: ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily. Cell 101, 789-800 (2000).
27. Standfuss, J. et al. Crystal structure of a thermally stable rhodopsin mutant. J. Mol. Biol. 372, 1179-1188 (2007).
28. Reyes, N., Ginter, C. & Boudker, O. Transport mechanismofabacterial homologue of glutamate transporters. Nature 462, 880-885 (2009).
29. Dawson, R. J., Hollenstein, K. & Locher, K. P. Uptake or extrusion: crystal structures of full ABC transporters suggest a common mechanism. Mol. Microbiol. 65, 250-257 (2007).
30. Goetz, B. A., Perozo, E. & Locher, K. P. Distinct gate conformations of the ABC transporter BtuCD revealed by electron spin resonance spectroscopy and chemical cross-linking. FEBS Lett. 583, 266-270 (2009).
31. Joseph, B., Jeschke, G., Goetz, B. A., Locher, K. P. & Bordignon, E. Transmembrane gate movements in the type II ATP-binding cassette (ABC) importer BtuCD-F during nucleotide cycle. J. Biol. Chem. 286, 41008-41017 (2011).
32. Sonnhammer, E. L., Eddy, S. R. & Durbin, R. Pfam: a comprehensive database of protein domain families based on seed alignments. Proteins 28, 405-420 (1997).
33. Borths, E. L., Locher, K. P., Lee, A. T. & Rees, D. C. The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter. Proc. Natl Acad. Sci. USA 99, 16642-16647 (2002).
34. Karpowich, N. K., Huang, H. H., Smith, P. C. & Hunt, J. F. Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding. J. Biol. Chem. 278, 8429-8434 (2003).
35. Jardetzky, O. Simpleallosteric model for membrane pumps. Nature 211, 969-970 (1966).
36. Hollenstein, K., Frei, D. C. & Locher, K. P. Structure of an ABC transporterincomplex with its binding protein. Nature 446, 213-216 (2007)
37. Locher, K. P. Review. Structure and mechanism of ATP-binding cassette transporters. Phil. Trans. R. Soc. Lond. B 364, 239-245 (2009).
38. Patzlaff, J. S., van der Heide, T. & Poolman, B. The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuA. J. Biol. Chem. 278, 29546-29551 (2003).
39. Chifflet, S., Torriglia, A., Chiesa, R. & Tolosa, S. A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: application to lens ATPases. Anal. Biochem. 168, 1-4 (1988).
40. Geertsma, E. R., Nik Mahmood, N. A., Schuurman-Wolters, G. K. & Poolman, B. Membrane reconstitutionofABC transporters and assaysoftranslocator function. Nature Protocols 3, 256-266 (2008).
41. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486-501 (2010).
42. Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D 66, 213-221 (2010).
43. Strong, M. et al. Toward the structural genomics of complexes: crystal structure of a PE/PPE protein complex from Mycobacterium tuberculosis. Proc. Natl Acad. Sci. USA 103, 8060-8065 (2006).
44. Dundas, J. et al. CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues. Nucleic Acids Res. 34, W116-W118 (2006).