Artificial microRNA-mediated knockdown of pyruvate formate lyase (PFL1) provides evidence for an active 3-hydroxybutyrate production pathway in the green alga Chlamydomonas reinhardtii

Journal of Biotechnology

Volumn 162, Issue 1, 2012, Pages 57-66

  Burgess, Steven J ^a,b   Tredwell, Gregory ^a   Molnàr, Attila ^b   Bundy, Jacob G ^a   Nixon, Peter J ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

3 Hydroxybutyrate; Artificial microRNA; Chlamydomonas; Hydrogen production; Pyruvate formate lyase

Indexed keywords

3-HYDROXYBUTYRATE; ACETYL-COA; ANAEROBIC METABOLISM; ANOXIC CONDITIONS; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; ETHANOL PRODUCTION; GREEN ALGA; METABOLIC PATHWAYS; MICROAEROBIC CONDITIONS; MICROAEROBICS; MICRORNAS; MRNA LEVEL; PRODUCTION OF HYDROGEN; PRODUCTION PATHWAYS; PYRUVATE FORMATE LYASE; PYRUVATES; RE-ROUTING; TARGET PROTEINS; TRANSFORMANTS;

ETHANOL; GALLIUM ALLOYS; HYDROGEN; HYDROGEN PRODUCTION; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SULFUR;

RNA;

3 HYDROXYBUTYRIC ACID; ACETIC ACID; ACETYL COENZYME A; ALCOHOL; FORMIC ACID; HYDROGEN; LACTIC ACID; LYASE; MESSENGER RNA; MICRORNA; PFL1 GENE; PYRUVATE FORMATE LYASE 1; PYRUVIC ACID; SULFUR; UNCLASSIFIED DRUG;

ALCOHOL PRODUCTION; ANAEROBIC METABOLISM; ANOXIA; ARTICLE; BIOTRANSFORMATION; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; DOWN REGULATION; ENZYME MECHANISM; EXCRETION; GENE; GREEN ALGA; LIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TECHNOLOGY;

3-HYDROXYBUTYRIC ACID; ACETYLTRANSFERASES; ANAEROBIOSIS; CHLAMYDOMONAS REINHARDTII; GENE KNOCKDOWN TECHNIQUES; HYDROGEN; METABOLIC NETWORKS AND PATHWAYS; MICRORNAS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; STRESS, PHYSIOLOGICAL; SULFUR;

CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA;

EID: 84867660300     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.05.010     Document Type: Article

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