Comparison of different interpretation strategies for low template DNA mixtures

Forensic Science International: Genetics

Volumn 6, Issue 6, 2012, Pages 716-722

  Pfeifer, Céline M ^a   Klein Unseld, Rachel ^a   Klintschar, Michael ^b   Wiegand, Peter ^a  

^a UNIVERSITY OF ULM   (Germany)

^b Institute of Legal Medicine   (Germany)

Author keywords

Composite; Consensus; Drop in; Drop out; Interpretation; Low template DNA; Mixture

Indexed keywords

DNA;

ALLELE; CONFERENCE PAPER; CONTROLLED STUDY; DNA FINGERPRINTING; DNA TEMPLATE; GENE AMPLIFICATION; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALLELES; DNA; DNA DEGRADATION, NECROTIC; DNA FINGERPRINTING; HUMANS; MICROSATELLITE REPEATS; MULTIPLEX POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 84867572656     PISSN: 18724973     EISSN: 18780326     Source Type: Journal    
DOI: 10.1016/j.fsigen.2012.06.006     Document Type: Conference Paper

References (21)

1. B. Budowle, A.J. Eisenberg, and A. van Daal Validity of low copy number typing and applications to forensic science Croat. Med. J. 50 2009 207 217
2. S. Cowen, P. Debenham, A. Dixon, S. Kutranov, J. Thomson, and K. Way An investigation of the robustness of the consensus method of interpreting low-template DNA profiles Forensic Sci. Int. Genet. 5 2011 400 406
3. T. Caragine, R. Mikulasovich, J. Tamariz, E. Bajda, J. Sebestyen, H. Baum, and M. Prinz Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler Croat. Med. J. 50 2009 250 267
4. C.C.G. Benschop, C.P. van der Beek, H.C. Meiland, A.G.M. van Gorp, A.A. Westen, and T. Sijen Low template STR typing: effect of replicate number and consensus method on genotyping reliability and DNA database search results Forensic Sci. Int. Genet. 5 2011 316 328
5. D.J. Balding, and J. Buckleton Interpreting low template DNA profiles Forensic Sci. Int. Genet. 4 2009 1 10
6. P. Gill, J. Whitaker, C. Flaxman, N. Brown, and J. Buckleton An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA Forensic Sci. Int. 112 2000 17 40
7. P. Gill, C.H. Brenner, J.S. Buckleton, A. Carracedo, M. Krawczak, W.R. Mayr, N. Morling, M. Prinz, P.M. Schneider, and B.S. Weir DNA commission of the international society of forensic genetics: recommendations on the interpretation of mixtures Forensic Sci. Int. 160 2006 90 101
8. P. Gill, and J. Buckleton A universal strategy to interpret DNA profiles that does not require a definition of low-copy-number Forensic Sci. Int. Genet. 4 2010 221 227
9. T. Tvedebrink, P.S. Eriksen, H.S. Mogensen, and N. Morling Estimating the probability of allelic drop-out of STR alleles in forensic genetics Forensic Sci. Int. Genet. 3 2009 222 226
10. H. Kelly, J.-A. Bright, J. Curran, and J. Buckleton The interpretation of low level DNA mixtures Forensic Sci. Int. Genet. 6 2012 191 197
11. H. Haned, T. Egeland, D. Pontier, L. Pene, and P. Gill Estimating drop-out probabilities in forensic DNA samples: a simulation approach to evaluate different models Forensic Sci. Int. Genet. 5 2011 525 531
12. J.-A. Bright, P. Gill, and J. Buckleton Composite profiles in DNA analysis Forensic Sci. Int. Genet. 6 2012 317 321
13. B. Bekaert, A. Van Geystelen, N. Vanderheyden, M.H. Larmuseau, and R. Decorte Automating a combined composite-consensus method to generate DNA profiles from low and high template mixture samples Forensic Sci. Int. Genet. 2012 10.1016/j.fsigen.2012.02.001
14. ENFSI DNA Working Group, DNA database management: review and recommendations, 2011 [cited April 2012]. Available from: www.enfsi.eu/get-doc. php?uid=345.
15. ® ESX and ESI Systems: Meeting the New European Standard, 2009 [cited May 2011]. Available from: http://www.promega. com/resources/articles/profiles-in-dna/2009/the-powerplex-esx-and-esi-systems- meeting-the-new-european-standard/.
16. K. Muller, R. Klein, E. Miltner, and P. Wiegand Q8 - a short amplicon multiplex including the German DNA database systems Forensic Sci. Int. Genet. 1 2007 205 207
17. D.T. Chung, J. Drabek, K.L. Opel, J.M. Butler, and B.R. McCord A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets J. Forensic Sci. 49 2004 733 740
18. T.M. Clayton, J.P. Whitaker, R. Sparkes, and P. Gill Analysis and interpretation of mixed forensic stains using DNA STR profiling Forensic Sci. Int. 91 1998 55 70
19. B. Budowle, A.J. Onorato, T.F. Callaghan, A. Della Manna, A.M. Gross, R.A. Guerrieri, J.C. Luttman, and D.L. McClure Mixture interpretation: defining the relevant features for guidelines for the assessment of mixed DNA profiles in forensic casework J. Forensic Sci. 54 2009 810 821
20. C.S. Tomsey, M. Kurtz, B. Flowers, J. Fumea, B. Giles, and S. Kucherer Case work guidelines and interpretation of short tandem repeat complex mixture analysis Croat. Med. J. 42 2001 276 280
21. P.M. Schneider, R. Fimmers, W. Keil, G. Molsberger, D. Patzelt, W. Pflug, T. Rothämel, H. Schmitter, H. Schneider, and B. Brinkmann The German stain commission: recommendations for the interpretation of mixed stains Int. J. Legal. Med. 123 2009 1 5