Comparison of Golgi apparatus and endoplasmic reticulum proteins from livers of juvenile and aged rats using a novel technique for separation and enrichment of organelles

Journal of Biomolecular Techniques

Volumn 16, Issue 4, 2005, Pages 345-353

  Drahos, Kate L ^a   Tran, Hung Cuong ^a   Kiri, Ajay N ^a   Lan, WenKui ^a   McRorie, Donald K ^a   Horn, Marcus J ^a  

^a Alfa Wassermann Proteomic Technologies LLC   (United States)

Author keywords

Continuous flow ultracentrifugation; Endoplasmic reticulum; Golgi apparatus; Organelle enrichment; Organelle separation

Indexed keywords

EID: 84867548442     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Hunter TC, Andon NL, Koller A, et al. The functional proteomics toolbox: methods and applications. J Chromatogr B 2002;782:165-181.
2. Isaaq HJ. Application of separation technologies to proteomics research. Adv Prot Chem 2003;65:249-269.
3. Isaaq HJ, Conrads TP, Janini GM, et al. Methods for fractionation, separation an profiling of proteins. Electrophoresis 2002;23:3048-3061.
4. Bae S-H, Harris AG, Hains PG, et al. Strategies for the enrichment and identification of basic proteins in proteome projects. Proteomics 2003;3:569-579.
5. Hoving H, Voshol H, van Oostrum J. Towards high performance two-dimensional gel electrophoresis using ultrazoom gels. Electrophoresis 2000;21:2617-2621.
6. Görg A, Obermaier C, Boguth G, et al. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 2000;21:1037-1053.
7. Butt A, Davison MD, Smith GJ, et al. Chromatographic separations as a prelude to two-dimensional electrophoresis inproteomics analysis. Proteomics 2001;1:42-53.
8. Görg A, Boguth G, Köpf A, et al. Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels. Proteomics 2002;2:1652-1657.
9. Pasquali C, Fialka I, Huber LA. Subcellular fractionation, electromigration analysis and mapping of organelles. J Chromatogr B 1999;722:89-102.
10. Wrzeszczynski KO, Rost B. Annotating proteins from endoplasmic reticulum and Golgi apparatus in eukaryotic proteomes. Cell Mol Life Sci 2004;61:1341-1353.
11. Marsh BJ, Howell KE. The mammalian Golgi-complex debates. Nature Rev Mol Cell Biol 2002;3:789-795.
12. Taylor RS, Wu CC, Hays LG, et al. Proteomes of rat liver Golgi complex: Minor proteins are identified through sequential fractionation. Electrophoresis 2000;21:3441-3459.
13. Bell AW, Ward MA, Blackstock WP, et al. Proteomics characterization of abundant Golgi membrane proteins. J Biol Chem 2001;276:5152-5165.
14. Griffith OM. Techniques of Preparative, Zonal, and Continuous Flow Centrifugation, Beckman Instruments, Fullerton, CA, 1986:9.
15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-254.
16. Drahos KL, Kiri AN, Lan W, et al. Comparison of liver mitochondrial proteins from rats of different ages using a novel technique for fractionation and enrichment of organelles. A poster presented at the 44TH Annual Meeting for the American Society for Cell Biology, December 2004. Washington, DC.
17. Le Gall IM and Bendayan M. Possible association of Chaperonin 60 with secretory proteins in pancreatic acinar cells. J Histochem Cytochem 1996;44:743-749.
18. Borregaard N, Cowland JB. Granules of the human neutrophilic polymorhonuclear leukocyte. Blood 1997;89:3503-3521.
19. Thuveson M and Fries E. Intracellular proteolytic processing of the heavy chain of rat pre-α-inhibitor. J Biol Chem 1999;274:6741-6746.
20. Lankat-Buttgereit B, Tampé R. The transporter associated with antigen processing: function and implication in human disease. Physiol Rev 2002;82:187-204.
21. Hennig D, Scales SJ, Moreau A, et al. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem 1998;273:19602-19611.
22. Bashour A-M, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formimiminotransferase cyclodeaminase. J Biol Chem 1998;273:19612-19617.
23. Gao Y, Alvarez C, Nelson DS, et al. Molecular cloning, characterization and dynamics of rat formiminotransferase cyclodeaminase. J Biol Chem 1998;273:33850-33834.
24. Lundstrom-Ljung J, Birnbach U, Rupp K, et al. Two resident ER-proteins, CaBP1 and CaBP2, with thioredoxin domains, are substrates for thioredoxin reductase: comparison with protein disulfide isomerase. FEBS Lett 1995;357:305-308.
25. Zhong X, Shen Y, Ballar P, et al. AAA ATPase p97/valosin-containing protein interacts with gp78, a ubiquitin ligase for endoplasmic reticulum-associated degradation. J Biol Chem 2004;279:45676-45684.
26. Hiller MM, Finger A, Schweiger M, et al. ER degradation of a misfolded luminal protein by the the cytosolic ubiquitin-proteasome pathway. Science 1996, 273, 1725-1728.
27. Mayer RJ. The meteoric rise of regulated intracellular proteolysis. Nature Rev Mol Cell Biol 2000;1:145-148.
28. Ellgaard L, Helenius A. Quality control in the endoplasmic reticulum. Nature Rev Mol Cell Biol 2003;4:181-191.