A RAPID HPLC-DAD method for analysis of theaflavins using C 1 2 as stationary phase

Journal of Liquid Chromatography and Related Technologies

Volumn 35, Issue 16, 2012, Pages 2272-2279

  Rana, Ajay ^a   Singh, Harsh Pratap ^a  

^a CSIR INSTITUTE OF HIMALAYAN BIORESOURCE TECHNOLOGY   (India)

Author keywords

antioxidants; black tea; C 1 2; catechins; RP HPLC; theaflavins

Indexed keywords

ANTIOXIDANTS; BLACK TEA; C 1 2; CATECHINS; RP-HPLC; THEAFLAVINS;

THEAFLAVIN; THEAFLAVIN 3,3' DIGALLATE; THEAFLAVINE 3 GALLATE; THEAFLAVINE 3' GALLATE; UNCLASSIFIED DRUG;

ARTICLE; CALIBRATION; DRUG ISOLATION; DRUG PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; QUALITATIVE ANALYSIS; SOLID PHASE EXTRACTION; TEA; THEA NIGRA;

EID: 84867377923     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826076.2011.631257     Document Type: Article

References (14)

1. Harbowy, M. E.; Balentine, D. A. Tea Chemistry. Crit. Rev. Plant Sci. 1997, 16, 415-480.
2. Matsuo, Y.; Tanaka, T.; Kouno. I. A New Mechanism for Oxidation of Epigallocatechin and Production of Benzotropolone Pigments. Tetrahedron 2006, 62, 4774-4783.
3. Geissman, T. A. Chemistry of Flavonoid Compounds, Pergamon Press: Oxford, UK 1962, 468-512.
4. Runeckles, V. C.; Tso, T. C. Recent Advances in Phytochemistry, Vol. 5, Academic Press: New York, 1972, pp. 247-31.
5. Sharma, K.; Bari, S. S.; Singh, H. P. Biotransformation of Tea Catechins into Theaflavins with Immobilized Polyphenol Oxidase. J. Mol. Cat. B Enzy. 2009, 56, 253-258.
6. Apostolides, Z.; Balentine, D. A.; Harbowy, M. E.; Hara, Y.; Weisurger. J. H. Inhibition of PhIP Mutagenicity by Catechins, and by Theaflavins and Gallate Esters. Mutat. Res. 1997, 389, 167-172. (Pubitemid 27227801)
7. Halder, B.; Pramanick, S.; Mukhopadhyay, S.; Giri, A. K. Anticlastogenic Effects of Black Tea Polyphenols Theaflavins and Thearubigins in Human Lymphocytes In Vitro. Toxicology in Vitro. 2006, 20, 608-613. (Pubitemid 43841810)
8. Liang, Y. C.; Chen, Y. C.; Lin, Y. L.; Lin-Shiau, S. Y.; Ho, C. T.; Lin. J. K. Suppression of Extracellular Signals and Cell Proliferation by the Black Tea Polyphenol, Theaflavin-3, 30-Digallate. Carcinogenesis 1999, 20, 733-736.
9. Pan, M. H.; Lin-Shiau, S. Y.; Ho, C. T.; Lin, J. H. Comparative Studies on the Suppression of Nitric Oxide Synthase by Curcumin and Its Hydrogenated Metabolites through Down-regulation of IkB Kinase and NFkB Activation in Macrophages. Biochem. Pharm. 2000, 59, 357-367.
10. Sang, S.; Tian, S.; Jhoo, J.; Wang, H.; Stark, R. E.; Rosen, R. T.; Ho. C. T. Chemical Studies of the Antioxidant Mechanism of Theaflavins: Radical Reaction Products of Theaflavin 3, 30-Digallate with Hydrogen Peroxide. Tetrahedron Lett. 2003, 44, 5583-5587. (Pubitemid 36782584)
11. Nishimura, M.; Ishiyama, K.; Watanabe, A.; Kawano, S.; Miyase. T.; Sano, M. Determination of Theaflavins Including Methylated Theaflavins in Black Tea Leaves by Solid-Phase Extraction and HPLC Analysis. J. Agric. Food Chem. 2007, 55, 7252-7257. (Pubitemid 47492274)
12. Jhoo, J.; Lo, C. Y.; Li, S.; Sang, S.; Ang, C. Y. W.; Heinze, T. M.; Ho, C. T. Stability of Black Tea Polyphenol, Theaflavin, and Identification of Theanaphthoquinone as Its Major Radical Reaction Product. J. Agric. Food Chem. 2005, 53, 6146-6150. (Pubitemid 41126660)
13. Wang, Y.; Yang, X.; Li, K.; Li, C.; Li, L.; Huang, H. Simultaneous Determination of Theanine, Gallic Acid, Purine Alkaloids, Catechins, and Theaflavins in Black Tea Using HPLC. Int. J. Food Sci. Tech. 2010, 45, 1263-1269.
14. Neilson, A. P.; Green, R. J.; Wood, K. V.; Ferruzzi. M. G. High-Throughput Analysis of Catechins and Theaflavins by High Performance Liquid Chromatography with Diode Array Detection. J. Chromatogr. A 2006, 1132, 132-140. (Pubitemid 44528491)