NMR structural studies of thymosin α1 and β-thymosins

Annals of the New York Academy of Sciences

Volumn 1270, Issue 1, 2012, Pages 73-78

  Volk, David E ^a,b   Tuthill, Cynthia W ^c   Elizondo Riojas, Miguel Angel ^a,d   Gorenstein, David G ^a,b  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF TEXAS   (United States)

^c SciClone Pharmaceuticals Inc   (United States)

^d HOSPITAL UNIVERSITARIO DR JOSÉ ELEUTERIO GONZÁLEZ   (Mexico)

Author keywords

Immunogenic peptide hormone; Protein structure; Thymalfasin; Thymosin 1; Thymosin 4; Thymosin 9

Indexed keywords

THYMOSIN; THYMOSIN ALPHA1; THYMOSIN BETA4; THYMOSIN BETA9; TRIFLUOROETHANOL; UNCLASSIFIED DRUG; WATER;

ALPHA HELIX; AQUEOUS SOLUTION; ARTICLE; BETA TURN; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; DRUG STRUCTURE; ENVIRONMENT; HETERONUCLEAR SINGLE QUANTUM COHERENCE; HYDROGEN BOND; HYDROPHILICITY; HYDROPHOBICITY; IMMUNE SYSTEM; LYMPHOCYTE MEMBRANE; NUCLEAR MAGNETIC RESONANCE; PH; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; TEMPERATURE;

EID: 84867375008     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.2012.06656.x     Document Type: Article

References (30)

1. Goldstein, A.L, T.L. Low, M. McAdoo, et al. 1977. Thymosin α1: isolation and sequence analysis of an immunologically active thymosin polypeptide. Proc. Natl. Acad. Sci. USA 74: 725-729.
2. Iino, S., J. Toyota, H. Kumada, et al. 2005. The efficacy and safety of thymosin alpha-1 in Japanese patients with chronic hepatitis B; results from a randomized clinical trial. J. Viral Hepat. 12: 300-306.
3. You, J., L. Zhuang, H.-Y. Cheng, et al. 2006. Efficacy of thymosin alpha-1 and interferon alpha in treatment of chronic viral hepatitis B: a randomized controlled study. World J. Gastroenterol. 12: 715-721.
4. Andreone, P., C. Cursaro, A. Gramenzi, et al. In vitro effect of thymosin-α1 and interferon-α on Th1 and Th2 cytokine synthesis in patients with chronic hepatitis C. J. Viral Hepatitis 8: 194-201.
5. Kullavanuaya, P., S. Treeprasertsuk, D. Thong-Ngam, et al. 2001. The combined treatment of interferon alpha-2a and thymosin alpha 1 for chronic hepatitis C: the 48 weeks end of treatment results. J. Med. Assoc. Thai. 84: 462-468.
6. Mosoian, A., A. Teixeira, C.S. Burns, et al. 2010. Prothymosin-a inhibits HIV-1 via toll-like receptor 4-mediated type I interferon induction. Proc. Natl. Acad. Sci. USA 107: 10178-10183.
7. Mosoian, A., A. Teixeira, A.A. High, et al. 2006. Novel function of prothymosin alpha as a potent inhibitor of human immunodeficiency virus type 1 gene expression in primary macrophages. J. Virol. 80: 9200-9206.
8. Bozza, S., R. Gaziano, P. Bonifazi, et al. 2007. Thymosin alpha1 activates the TLR9/MyD88/IRF7-dependent murine cytomegalovirus sensing for induction of anti-viral responses in vivo. Int. Immunol. 19: 1261-70.
9. Segal, B.H. & T. J. Walsh. 2006. Current approaches to diagnosis and treatment of invasive Aspergillosis. Am. J. Respir. Crit. Care Med. 173: 707-717.
10. Romani, L., F. Bistoni, K. Perruccio, et al. 2006. Thymosin α1 activates dendritic cell tryptophan catabolism and established a regulatory environment for balance of inflammation and tolerance. Blood 108: 2265-2274.
11. Grottesi, A., M. Sette, A.T. Palamara, et al. 1998. The conformation of peptide thymosin a1 in solution and in a membrane-like environment by circular dichroism and NMR spectroscopy. A possible model for its interaction with the lymphocyte membrane. Peptides 19: 1731-1738.
12. Czisch, M., M. Schleicher, S. Hörger, W. Voelter & T.A. Holak. 1993. Conformation of thymosin beta 4 in water determined by NMR spectroscopy. Eur. J. Biochem. 218: 335-344.
13. Stoll, R., W. Voelter & T.A. Holak. 1997. Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy. Biopolymers 41: 623-634.
14. Elizondo-Riojas, M.-A., S.M. Chamow, C.W. Tuthill, et al. 2011. NMR structure of human thymosin alpha-1. Biochem. Biophys. Res. Commun. 416: 356-361.
15. Hannappel, E., S. Davoust & B.L. Horecker. 1982. Thymosins β8 and β9: two new peptides isolated from calf thymus homologous to thymosin β4. Proc. Natl. Acac. Sci. U.S.A. 79: 1708-1711.
16. Hutchinson, E.G. & J.M. Thornton. 1994. A revised set of potentials for beta turn formation in proteins. Prot. Sci. 3: 2207-2216.
17. Zarbock, J., H. Oschkinat, E. Hannapel, et al. 1990. The solution conformation of thymosin b4: a nuclear magnetic resonance and simulated annealing study. Biochemistry 29: 7814-7821.
18. Richardson, J.S. & D.C. Richardson. Amino acid preferences for specific amino acids at the ends of alpha helices. 1988. Science 240: 1648-1652.
19. Seale, J.W., S. Rajgopal & G.D. Rose. 1994. Sequence determinants of the capping box, a stabilizing motif af the N-termini of alpha helices. Protein Sci. 3: 1741-1745.
20. Aurora, R., R. Srinivasan & G.D. Rose. 1994. Rules for α-helix termination by glycine. Science 264: 1126-1130.
21. Schellman, J.A. 1958. The factors affecting the stability of hydrogen-bonded polypeptide structures in solution. J. Phys. Chem. 62: 1485-1494.
22. Serrano, L. & A.R. Fersht. 1989. Capping and alpha-helix stability. Nature 342: 296-299.
23. Safer, D., M. Elzinga & V.T. Nachmias. 1991. Thymosin beta 4 and Fx, an actin-sequestering peptide, are indistinguishable. J. Biol. Chem. 266: 4029-4032.
24. Weber, A., V.T. Nachmias, C.R. Pennise, et al. 1992. Interaction of thymosin beta 4 with muscle and platelet actin: implications for actin sequestration in resting platelets. Biochemistry 31: 6179-6185.
25. Cassimeris, L., D. Safer, V.T. Nachmias & S.H. Zigmond. 1992. Thymosin beta 4 sequesters the majority of G-actin in resting human polymorphonuclear leukocytes. J. Cell. Biol. 119: 1261-1270.
26. Paunola, E., P.K. Mattila & P. Lappalainen. 2002. WH2 domain: a small, versatile adapter for actin monomers. FEBS Lett. 513: 92-97.
27. Holmes, K.C., D. Popp, W. Gebhard & W. Kabsch. 1990. Atomic model of the actin filament. Nature 347: 44-49.
28. McLaughlin, P.J., J.T. Gooch, H.G. Mannherz & A.G. Weeds. 1993. Structure of gelsolin segment 1-actin complex and the mechanism of filament severing. Nature 364: 685-692.
29. Schutt, C.E., J.C. Myslik, M.D. Rozycki, et al. 1993. The structure of crystalline profiling-β-actin. Nature 365: 810-816.
30. Domanski, M., M. Hertzog, J. Coutant, et al. 2004. Coupling of folding and binding of thymosin β4 upon interaction with monomeric actin monitored by nuclear magnetic resonance. J. Biol. Chem. 279: 23637-23645.