Multiplex PCR assay for the detection of Pantoea stewartii subsp. stewartii using species-specific genetic markers

Australasian Plant Pathology

Volumn 41, Issue 5, 2012, Pages 559-564

  Thapa, Shree Prasad ^a   Park, Duck Hwan ^a   Wilson, Calum ^b   Hur, Jang Hyun ^a   Lim, Chun Keun ^a  

^a Kangwon National University   (South Korea)

^b UNIVERSITY OF TASMANIA   (Australia)

Author keywords

Genetic marker; Multiplex PCR; P. stewartii subsp. stewartii; Specific detection

Indexed keywords

BACTERIA (MICROORGANISMS); PANTOEA; PANTOEA STEWARTII SUBSP. STEWARTII; ZEA MAYS;

EID: 84867036176     PISSN: 08153191     EISSN: 14486032     Source Type: Journal    
DOI: 10.1007/s13313-012-0123-9     Document Type: Article

References (16)

1. Bertolini E, Olmos A, López MM, Cambra M (2003) Multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four RNA viruses and Pseudomonas savastanoi pv. savastanoi in olive trees. Phytopathology 93:286-292 (Pubitemid 36251374)
2. Blakemore EJA, Reeves JC, Ball SFL (1992) Polymerase chain reaction used in the development of a DNA probe to identify Erwinia stewartii, a bacterial pathogen of maize. Seed Sci Technol 20:331-335
3. Block CC, Hill JH, McGee DC (1999) Relationship between lateseason severity of Stewart's Bacterial Wilt and seed infection in maize. Plant Dis 83:527-530
4. Coplin DL, Majerczak DR (1990) Extracellular polysaccharide genes in Erwinia stewartii: directed mutagenesis and complementation analysis. Mol Plant Microbe Interact 3:286-292
5. Coplin DL, Majerczak DR, Zhang Y, Kim WS, Jock S, Geider K (2002) Identification of Pantoea stewartii subsp. stewartii by PCR and strain differentiation by PFGE. Plant Dis 86:304-311 (Pubitemid 34191083)
6. Dolph PJ, Majerczak DR, Coplin DL (1988) Characterization of a gene cluster for exopolysaccharide biosynthesis and virulence in Erwinia stewartii. J Bacteriol 170:865-871
7. Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE (2000) Multiplex-PCR: optimization and application in diagnostic virology. Clin Microbiol Rev 13:559-570
8. Frederick RD, Ahmad M, Majerczak DR, Arroyo-Rodríguez AS, Manulis S, Coplin DL (2001) Genetic organization of the Pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpA, hrpC, hrpN and wtsE operons. Mol Plant Microbe Interact 14:1213-1222 (Pubitemid 32972425)
9. Ham JH, Majerczak DR, Ewert S, Sreerekha MV, Mackey D, Coplin D (2008) WtsE, an AvrE-family type III effector protein of Pantoea stewartii subsp. stewartii, causes cell death in non-host plants. Mol Plant Pathol 9:633-643
10. Markoulatos P, Siafakas N, Moncany M (2002) Multiplex polymerase chain reaction: a practical approach. J Clin Lab Anal 16:47-51
11. Michener PM, Pataky JK, White DG (2002) Transmission of Erwinia stewartii from plants to kernels and reactions of corn hybrids to Stewart's wilt. Plant Dis 86:167-172 (Pubitemid 34115416)
12. Özdemir Z (2005) Development of a multiplex PCR assay for concurrent detection of Clavibacter michiganensis ssp. michiganensis and Xanthomonas axonopodis pv. vesicatoria. Plant Pathol J 4:133-137
13. Sharma NK, Rees CE, Dodd CE (2000) Development of a singlereaction multiplex PCR toxin typing assay for Staphylococcus aureus strains. Appl Environ Microbiol 66:1347-1353 (Pubitemid 30185600)
14. Tambong T, Mwange KN, Bergeron M, Ding T, Mandy F, Reid LM, Zhu X (2008) Rapid detection and identification of the bacterium Pantoea stewartii in maize by TaqMan® real-time PCR assay targeting the cpsD gene. J Appl Microbiol 104:1525-1537 (Pubitemid 351533633)
15. Willson WJ, Wiedmann M, Dillard HR, Batt CA (1994) Identification of Erwinia stewartii by a ligase chain reaction assay. Appl Environ Microbiol 60:278-284 (Pubitemid 24019949)
16. Wilson K (1989) Preparation of genomic DNA from bacteria. In: Ausubel FM, Brent R, Kinston RE (eds) Current protocols in molecular biology. Wiley, NY, pp 2.4.1-2.4.5